In vitro neurite extension by granule neurons is dependent upon astroglial-derived fibroblast growth factor

Hatten, Mary E.; Lynch, Marion H.; Rydel, Russell E.; Sanchez, Janet; Joseph‐Silverstein, Jacquelyn; Moscatelli, David; Rifkin, Daniel B.

doi:10.1016/0012-1606(88)90211-4

Cited by 408 publications

(192 citation statements)

References 28 publications

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“…P8 cerebella were harvested into PBS and assessed for YFP. Cerebellar GNPs were purified by percoll fractionation (3,42) and used for ChIP or qPCR. Rosa Gli1-FLAG/Gli1-FLAG ;Math1-Cre/+ mice were bred to Ptch1 +/− (13) mice.…”

Section: Methodsmentioning

confidence: 99%

Hedgehog pathway-regulated gene networks in cerebellum development and tumorigenesis

Lee

Ouyang³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

113

101

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Many genes initially identified for their roles in cell fate determination or signaling during development can have a significant impact on tumorigenesis. In the developing cerebellum, Sonic hedgehog (Shh) stimulates the proliferation of granule neuron precursor cells (GNPs) by activating the Gli transcription factors. Inappropriate activation of Shh target genes results in unrestrained cell division and eventually medulloblastoma, the most common pediatric brain malignancy. We find dramatic differences in the gene networks that are directly driven by the Gli1 transcription factor in GNPs and medulloblastoma. Gli1 binding location analysis revealed hundreds of genomic loci bound by Gli1 in normal and cancer cells. Only one third of the genes bound by Gli1 in GNPs were also bound in tumor cells. Correlation with gene expression levels indicated that 116 genes were preferentially transcribed in tumors, whereas 132 genes were target genes in both GNPs and medulloblastoma. Quantitative PCR and in situ hybridization for some putative target genes support their direct regulation by Gli. The results indicate that transformation of normal GNPs into deadly tumor cells is accompanied by a distinct set of Gli-regulated genes and may provide candidates for targeted therapies.

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Section: Methodsmentioning

confidence: 99%

Hedgehog pathway-regulated gene networks in cerebellum development and tumorigenesis

Lee

Ouyang³

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

113

101

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“…The precise molecular nature ofthe factor or factors responsible is not known, although protein degradation studies indicate that they are proteins or require protein cofactors. While previously identified soluble peptide growth factors have been observed to promote neurite outgrowth from CNS neurons, none ofthese studies has reported selective effects on the growth of either axons or dendrites (Hatten et al, 1988;Rousselet et al, 1988;Lein and Higgins, 1989;Mattson et al, 1989).…”

Section: Androglia Influence the Number Of Primary Dendritesmentioning

confidence: 96%

“…A variety of studies indicate that neurotrophic factors support neurite growth from central neurons (Walicke et al, 1986;Hatten et al, 1988;Mattson et al, 1989;Kawaja and Gage, 199 1). Our results indicate that, in part, dendrite growth is modulated by a diffusible factor.…”

Section: Androglia Influence the Number Of Primary Dendritesmentioning

confidence: 99%

“…There is considerable evidence that glial cells secrete diffusible neurite-promoting factors (Hatten et al, 1988;Rousselet et al, 1988Rousselet et al, , 1990Qian et al, 1992). For example, media conditioned on homotypic glia can enhance polarity of embryonic mouse mesencephalic neurons in primary culture (Rousselet et al, 1988(Rousselet et al, , 1990.…”

Section: Androglia Influence the Number Of Primary Dendritesmentioning

confidence: 99%

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Regional differences in glial-derived factors that promote dendritic outgrowth from mouse cortical neurons in vitro

Roux

Reh

1994

J. Neurosci.

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To determine whether glia from different CNS regions differ in their ability to support axons or dendrites, embryonic (E18) mouse cortical neurons were cocultured with early postnatal (P4) rat astroglial derived from cortex, retina, olfactory bulb, mesencephalon, striatum, and spinal cord. After 5 d in vitro, axon and dendrite outgrowth from isolated neurons was quantified with double-labeling immunohistochemical techniques. Whereas axonal growth was similar on the various monolayers, total primary dendritic outgrowth was nearly threefold greater on glia derived from the cortex, retina, and olfactory bulb than on glia derived from mesencephalon, striatum, or spinal cord. This effect was principally on the number of primary dendrites rather than the elongation of individual dendrites. Similar morphological differences were observed when cortical neurons were grown on polylysine in a noncontact coculture system with glia continuously conditioning the media. This selective promotion of dendrite growth was independent of neuron survival. These results indicate that there are regional differences in the ability of CNS glia to support dendritic growth and that this effect is due, in part, to release of a diffusable factor.

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“…It has been reported recently that FGFs promote in vitro survival ofneurons from different parts ofthe nervous system (12)(13)(14)(15)(16). In addition, neuronal survival-enhancing effects have also been seen after lesion in vivo (17,18).…”

mentioning

confidence: 98%

Acidic and basic fibroblast growth factors are survival factors with distinctive activity in quiescent BALB/c 3T3 murine fibroblasts.

Tamm

Kikuchi

Zychlinsky

1991

Proc. Natl. Acad. Sci. U.S.A.

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Acidic and basic fibroblast growth factors are survival factors with distinctive activity in quiescent BALB/c 3T3 murine fibroblasts (protein synthesis/protein kinase C/platelet-derived growth factor/membrane vesiculation/nuclear condensation and disruption) IGOR TAMM*t, TOYOKO KIKUCHI*, AND . We now show that FGF possesses marked cell survival-enhancing activity distinctive from that of PDGF. Both acidic FGF (aFGF) and basic FGF (bFGF) markedly enhance short-term (3-hr) survival of quiescent cells. bFGF is the more active of the two factors and shows marked long-term (20-hr) survival-promoting activity alone, whereas aFGF requires heparin for long-term activity. Protection by bFGF or aFGF plus heparin is not associated with cell-cycle traverse into S phase. Both the short-term (3-hr) and long-term (20-hr) protective actions of aFGF and bFGF critically depend on protein synthesis, whereas those of PDGF do not. The accumulated evidence shows that several growth factors can contribute to maintenance of the integrity of quiescent murine fibroblasts and that their action can involve protein kinase Aand C-mediated processes as well as protein synthesis. Different growth factors display distinctive modes of action.

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In vitro neurite extension by granule neurons is dependent upon astroglial-derived fibroblast growth factor

Cited by 408 publications

References 28 publications

Hedgehog pathway-regulated gene networks in cerebellum development and tumorigenesis

Hedgehog pathway-regulated gene networks in cerebellum development and tumorigenesis

Regional differences in glial-derived factors that promote dendritic outgrowth from mouse cortical neurons in vitro

Acidic and basic fibroblast growth factors are survival factors with distinctive activity in quiescent BALB/c 3T3 murine fibroblasts.

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