In vitro nuclear reconstitution could be induced in a plant cell‐free system

Zhao, Yun; Liu, Xiaoling; Wu, Min; Tao, Wei; Zhai, Zhonghe

doi:10.1016/s0014-5793(00)01938-4

Cited by 9 publications

(14 citation statements)

References 27 publications

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“…In plants, the breakdown and reassembly of the nuclear envelope takes place according to a scenario similar to the one described with animal cells. Indeed, the nuclear envelope can form from cytosolic extracts of plant cell suspensions around demembranated Xenopus sperm chromatin [92,93], and conversely, animal extracts are able to assemble a nuclear envelope structure around plant chromatin [82,94]. Therefore, one may postulate that RTN will also intervene in the reassembly of the nuclear envelope in plant cells as they do in animal cells.…”

Section: Nuclear Envelope Growthmentioning

confidence: 99%

Functions of reticulons in plants: What we can learn from animals and yeasts

Nziengui

Schoefs

2008

Cell. Mol. Life Sci.

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Reticulons (RTNs) are membrane-spanning proteins sharing a typical domain named reticulon homology domain (RHD). RTN genes have been identified in all eukaryotic organisms examined so far, and the corresponding proteins have been found predominantly associated to the endoplasmic reticulum membranes. In animal and yeast, in which knowledge of the protein family is more advanced, RTNs are involved in numerous cellular processes such as apoptosis, cell division and intracellular trafficking. Up to now, a little attention has been paid to their plant counterparts, i.e., RTNLBs. In this review, we summarize the data available for RTNLB proteins and, using the data obtained with animal and yeast models, several functions for RTNLBs in plant cells are proposed and discussed.

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Section: Nuclear Envelope Growthmentioning

confidence: 99%

Functions of reticulons in plants: What we can learn from animals and yeasts

Nziengui

Schoefs

2008

Cell. Mol. Life Sci.

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“…And some heterologous sperm nuclei with far relationship to the species from which the extract were made could be decondensed in the Xenopus laevis egg extract or cell-free extract of carrot [10,31]. We observed that both the red carp and gibel carp sperm nuclei incubated in the red carp egg extracts decondensed and swelled obviously, and underwent pronucleus formation (Fig 1).…”

Section: Discussionmentioning

confidence: 82%

“…The sperm nuclear decondensation is obviously a critical early step in embryonic developmental process, necessary for duplication and ultimate transcription of the male genome [2]. Plenty of information on the early events of sperm nuclei after fertilization, including swelling, chromatin decondensation, DNA synthesis, and nuclear envelope assembly, have been obtained from the studies in cell-free systems based on Xenopus eggs extracts [3][4][5][6], and cytosol extracts of Drosophila embryo [7], CHO cells [8], sea urchin eggs [9], and carrot cell [10].…”

Section: Introductionmentioning

confidence: 99%

Comparative studies on in vitro sperm decondensation and pronucleus formation in egg extracts between gynogenetic and bisexual fish

Gui

2003

Cell Res

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A cell-free system based upon the egg extracts from gynogenetic gibel carp (Carassius auratus gibelio) or bisexual red common carp (Cyprinus carpio red variety) was developed to investigate developmental behaviors of the demembranated sperm nuclei. Both red common carp and gibel carp sperm nuclei could decondense fully and form pronuclei in the red common carp egg extracts. Gibel carp sperm nuclei could also decondense fully and form pronuclei in the gibel carp egg extracts, but red common carp sperm nuclei could not decondense sufficiently in the same extracts. The significant differences of morphological changes were further confirmed by ultrastructural observation of transmission electron microscopy. The data further offer cytological evidence for gonochoristic reproduction in the gynogenetically reproducing gibel carp. In addition, the sperm nuclei in vitro decondensation is dependent on the pH in the extracts, and the decondensed efficiency is optimal at pH 7. However, no DNA replication was observed in the two kinds of egg extracts during the incubation period of the sperm nuclei. It is suggested that the egg extracts prepared from the gynogenetic gibel carp should be a valid in vitro system for studying molecular mechanism on gynogenesis and reproduction mode diversity in fish.

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“…CS-100 is a good cell-free system for studying the apoptotic mechanism [16][17][18]. We succeeded in reconstructing nuclei in the system [19], and have shown that nuclei from either animals or plants can be effectively triggered into apoptosis [17,18], even though the system is from the cytosol of carrot cells. We hope that the CS-100 will be a powerful tool for studying evolutionary relationships between plants and other species.…”

Section: Research Articlementioning

confidence: 99%

“…To understand the structural basis of apoptotic morphology, we characterized nuclear apoptosis in a cell-free system, a useful experimental system for research into the apoptotic mechanism [4,[14][15][16][17][18]. We have developed a carrot cytosol cell-free system named CS-100 [17][18][19]. Cytochrome c is responsible for mediating the apoptotic downstream pathway from mitochondria [4,20,21].…”

mentioning

confidence: 99%

Analysis of nuclear apoptotic process in a cell-free system

Zhao¹,

Wu²,

Shen³

et al. 2001

CMLS, Cell. Mol. Life Sci.

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We report an analysis of the apoptotic process of mouse liver nuclei induced in a cell-free carrot cytosol system by cytochrome c. Typical characteristics of apoptosis were observed, such as chromatin condensation, margination, apoptotic bodies and DNA ladders. Furthermore, transmission and scanning electron microscope analysis of the apoptotic nuclei detected chromatin-free nuclear vesicles before apoptotic bodies appeared at a comparatively late phase. When AC-YVAD-CHO, an inhibitor of caspase 6, was introduced into the system, these vesicles and apoptotic bodies disappeared completely within our study sections. We confirmed the results using whole-mount electron microscopy, and found that although the nuclear lamina was destroyed early, the nuclear matrix largely remained intact during the course of apoptosis. The nuclear matrix played an important role in maintaining the integrity of apoptotic cells and connecting the apoptotic bodies and apoptotic nucleus.

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In vitro nuclear reconstitution could be induced in a plant cell‐free system

Cited by 9 publications

References 27 publications

Functions of reticulons in plants: What we can learn from animals and yeasts

Functions of reticulons in plants: What we can learn from animals and yeasts

Comparative studies on in vitro sperm decondensation and pronucleus formation in egg extracts between gynogenetic and bisexual fish

Analysis of nuclear apoptotic process in a cell-free system

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