In vitro nutrition of anthurium (Anthurium andreanum Linden var. Tropical) in three culture systems

Abstract

Anthurium is a very important ornamental plant in the market. However, its traditional reproduction presents problems, therefore in vitro culture represents an alternative for its propagation. In the in vitro plant tissue culture, the nutrients are provided by the media, but information on the in vitro nutrition for anthurium is scarce. The objective of this research was to evaluate plant growth characteristics, nutrient content in plant tissues and nutrient removal from the culture medium in three in vitro culture systems: semi-solid, partial immersion and the RITA® bioreactor. The data were analyzed, using an ANOVA and compared with Tukey tests, using the statistical software R-STUDIO. The highest length of shoots and roots were observed with partial immersion; the number of leaves, shoots and roots did not show differences with the RITA® bioreactors. The highest tissue content of nutrients occurred in plants cultivated in the RITA® bioreactor. For the content of nutrients in the culture medium, the highest one was observed in the RITA® bioreactor, which indicates a most efficient use of nutrients, due to the greater contact of explants with the media and a best gas exchange. Therefore, the RITA® bioreactor represents the most viable option for anthurium plants production.