In-vitro Optimization of Nanoparticle-Cell Labeling Protocols for In-vivo Cell Tracking Applications

Abstract

Recent advances in theranostic nanomedicine can promote stem cell and immune cell-based therapy. Gold nanoparticles (GNPs) have been shown to be promising agents for in-vivo cell-tracking in cell-based therapy applications. Yet a crucial challenge is to develop a reliable protocol for cell upload with, on the one hand, sufficient nanoparticles to achieve maximum visibility of cells, while on the other hand, assuring minimal effect of particles on cell function and viability. Previous studies have demonstrated that the physicochemical parameters of GNPs have a critical impact on their efficient uptake by cells. In the current study we have examined possible variations in GNP uptake, resulting from different incubation period and concentrations in different cell-lines. We have found that GNPs effectively labeled three different cell-lines - stem, immune and cancer cells, with minimal impairment to cell viability and functionality. We further found that uptake efficiency of GNPs into cells stabilized after a short period of time, while GNP concentration had a significant impact on cellular uptake, revealing cell-dependent differences. Our results suggest that while heeding the slight variations within cell lines, modifying the loading time and concentration of GNPs, can promote cell visibility in various nanoparticle-dependent in-vivo cell tracking and imaging applications.

Magnesium Oxide in Nanodimension: Model for MRI and Multimodal Therapy

“…Many promising techniques still figured out several issues like limitations of reliable protocols such as appropriate labelling, interaction of hydrophobic and hydrophilic nanoparticles, and proper visibility of cells from data for disease recognition, to achieve a minimal damaging effect in a healthy site [61][62][63][64].…”

Effective Labeling of Primary Somatic Stem Cells with BaTiO3 Nanocrystals for Second Harmonic Generation Imaging

“…The intracellular accumulation of SHG nanoprobes in quiescent and proliferative hematopoietic stem and progenitor cells occurs mainly through clathrin‐mediated endocytosis. Importantly, inferred from TEM data analysis, high contrast labeling was achieved with at least one order of magnitude less intracellular nanocrystals (less than 100) than previously reported . Once internalized, SHG nanoprobes persist without active export and become only diluted in stem cells upon cell division.…”

Advances in imaging strategies for in vivo tracking of exosomes

“…CT provides superior visualization of bone structures due to the inherent contrast between electron-dense bones and the more permeable surrounding soft tissues (Meir, Shamalov, et al, 2017;Shwartz et al, 2017). Therefore, CT has been used for cell tracking while combined with nanoparticles as labeling agents (Betzer et al, 2014;Betzer et al, 2015;Betzer, Meir, Motiei, Yadid, & Popovtzer, 2017;Betzer, Shilo, Motiei, & Popovtzer, 2019;Betzer, Shilo, et al, 2017;Hazkani et al, 2017;Meir, Betzer, et al, 2017;Meir, Betzer, Barnoy, Motiei, & Popovtzer, 2018;Popovtzer, 2014;Shilo et al, 2015;Shwartz et al, 2017).…”