In vitro organogenesis and plant regeneration of Passiflora xishuangbannaensis, a species with extremely small populations

Meng, Yuanyuan; Song, Shijie; Landrein, Sven

doi:10.1016/j.gecco.2021.e01836

Cited by 5 publications

(4 citation statements)

References 44 publications

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“…The result highlighted the importance of balances among PGRs, especially between cytokinins and auxins. In general, a low concentration of cyrokinins and a high concentration of auxins results in callus formation [15,90,91]. In line with our results, Huh et al, [83] that the maximum callus formation in P. edulis was obtained from leaf explants cultured in the medium containing a high concentration of auxin (2,4-D) with a low concentration of cytokinin.…”

Section: Plos Onesupporting

confidence: 91%

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Machine learning-mediated Passiflora caerulea callogenesis optimization

Jafari,

Daneshvar

2024

PLoS ONE

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Callogenesis is one of the most powerful biotechnological approaches for in vitro secondary metabolite production and indirect organogenesis in Passiflora caerulea. Comprehensive knowledge of callogenesis and optimized protocol can be obtained by the application of a combination of machine learning (ML) and optimization algorithms. In the present investigation, the callogenesis responses (i.e., callogenesis rate and callus fresh weight) of P. caerulea were predicted based on different types and concentrations of plant growth regulators (PGRs) (i.e., 2,4-dichlorophenoxyacetic acid (2,4-D), 6-benzylaminopurine (BAP), 1-naphthaleneacetic acid (NAA), and indole-3-Butyric Acid (IBA)) as well as explant types (i.e., leaf, node, and internode) using multilayer perceptron (MLP). Moreover, the developed models were integrated into the genetic algorithm (GA) to optimize the concentration of PGRs and explant types for maximizing callogenesis responses. Furthermore, sensitivity analysis was conducted to assess the importance of each input variable on the callogenesis responses. The results showed that MLP had high predictive accuracy (R2 > 0.81) in both training and testing sets for modeling all studied parameters. Based on the results of the optimization process, the highest callogenesis rate (100%) would be obtained from the leaf explant cultured in the medium supplemented with 0.52 mg/L IBA plus 0.43 mg/L NAA plus 1.4 mg/L 2,4-D plus 0.2 mg/L BAP. The results of the sensitivity analysis showed the explant-dependent impact of the exogenous application of PGRs on callogenesis. Generally, the results showed that a combination of MLP and GA can display a forward-thinking aid to optimize and predict in vitro culture systems and consequentially cope with several challenges faced currently in Passiflora tissue culture.

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Section: Plos Onesupporting

confidence: 91%

“…The result highlighted the importance of balances among PGRs, especially between cytokinins and auxins. In general, a low concentration of cyrokinins and a high concentration of auxins results in callus formation [ 15 , 90 , 91 ]. In line with our results, Huh et al ., [ 83 ] that the maximum callus formation in P .…”

Section: Discussionmentioning

confidence: 99%

Machine learning-mediated Passiflora caerulea callogenesis optimization

Jafari,

Daneshvar

2024

PLoS ONE

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“…Micropropagation is an important and effective method of propagation used for many plant species (Meng et al 2021). However, it is still stressful, which can limit the successful establishment of plantlets upon transfer to ex vitro and in vivo conditions (Gago et al 2014).…”

Section: Resultsmentioning

confidence: 99%

Micro‐morpho‐anatomical transitions at various stages of in vitro development of Crinum malabaricum Lekhak and Yadav: A critically endangered medicinal plant

et al. 2022

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Crinum malabaricum Lekhak & Yadav is a recently discovered and critically endangered aquatic bulbous plant of the family Amaryllidaceae. It gained attention as a wild source of the acetylcholinesterase inhibiting alkaloid 'galanthamine' used to treat Alzheimer and Parkinson diseases. The bulbs of this plant contain the highest amount of galanthamine among Crinum species.• In vitro regeneration systems were developed to produce quality uniform plantlets of C. malabaricum. Bright field light microscopy was used to analyse micro-morphoanatomical developments taking place in the leaves and roots during in vitro, ex vitro and in vivo transitions of plantlets.• Leaves and roots of plants raised in vitro possessed a higher degree of microscopic structural anomalies, such as underdeveloped epicuticular wax deposition, immature and non-functional stomata, more aquiferous parenchyma with a reduced lumen. Roots developed in vitro were characterized by extremely large, uneven cortical cells and reduced intercellular spaces. The vascular tissues were under-developed and only primary vascular tissues were observed. As a result of ex vitro acclimation, there was a significant acceleration in the improvement of tissue systems in leaves and roots. Such plantlets can tolerate elevated temperatures and light under in vivo conditions.• Thus, the microscopic evaluation of the structural trajectory in different stages of plantlet development provides an understanding of the acclimation process and structural adaptations, which could help enhance survival of in vitro raised plantlets under ex vitro and in vivo conditions.

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“…The first study was performed in 1966 on P. caerulea, using nodal explants as the initiation material [56]. Since then, studies showing various micropropagation techniques in Passiflora have been on the rise [22,29,39,42,[57][58][59][60][61]. All these studies show that organogenesis remains the main regeneration path for this species [53].…”

Section: Discussionmentioning

confidence: 99%

Plant Regeneration Protocol for Recalcitrant Passionflower (Passiflora quadrangularis L.)

et al. 2022

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This research was designed to provide the first protocol to establish an efficient solution for direct organogenesis regeneration in Passiflora quadrangularis using nodal explants from young shoots. Passifloraceae tissue culture has been associated with problems such as recalcitrance, sensitivity to ethylene accumulation and browning of explants due to the presence of phenols in the tissues. Due to the high rate of endogenous contamination of the explants, a preliminary experiment was performed. The best results of surface sterilization were obtained using the pretreatment with 70% EtOH, 1 min and 50% NaOCl, 10 min along with the treatment of Rifampicin 15 µg/mL and Benomyl 2 g/L. The effects of plant growth regulators on the induction of direct organogenesis, multiplication of shoots in subcultures and in vitro rooting were evaluated. Additional compounds such as AgNO3 and Pluronic F-68 were added to the culture media in order to reduce the effects of phenols and the sudden browning of the explants. Shoot proliferation increased to the sixth subculture after which it decreased. A maximum of 7.17 shoots were obtained from one shoot on Murashige and Skoog (MS) medium supplemented with 2 mg/L 6-benzylaminopurine and 1 mg/L thidiazuron. Supplementation of ½ MS medium with 1 mg/L 1-naphthaleneacetic acid was conducing to root formation in 61.11% of shoots. After acclimatization, the plants showed vigorous growth, green leaves, and well-developed roots. Although this species has previously shown difficulty in in vitro propagation, this protocol established based on the results proved to be efficient and reproducible.

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In vitro organogenesis and plant regeneration of Passiflora xishuangbannaensis, a species with extremely small populations

Cited by 5 publications

References 44 publications

Machine learning-mediated Passiflora caerulea callogenesis optimization

Machine learning-mediated Passiflora caerulea callogenesis optimization

Micro‐morpho‐anatomical transitions at various stages of in vitro development of Crinum malabaricum Lekhak and Yadav: A critically endangered medicinal plant

Plant Regeneration Protocol for Recalcitrant Passionflower (Passiflora quadrangularis L.)

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