In vitro ovicidal activity of Peganum harmala seeds extract on the eggs of Fasciola hepatica

Abstract

seeds extract has been previously reported to have antimicrobial and other medicinal properties. The aim of this study was to evaluate the ovicidal activity of the methanolic extract of seeds against the eggs of The phenolic compounds of the methanolic extract of seeds were identified by HPLC analysis. Catechin, rutin, p-Coumaric acid, chloregenic acid and hesperetin were found to be the major phenolic compounds. eggs were collected from the gall bladder of naturally infected sheep. The eggs were exposed to two concentrations of seeds extract (1 and 3 mg/mL) for 24 and 48 h. To investigate the effect of the seeds extract on the miracidial formation, the treated eggs were incubated at 28 °C for 14 days. The results indicated that eggs were susceptible to the methanolic extract of seeds. Following 24 h exposure of the eggs to seeds extract with concentrations of 1 and 3 mg/mL, the miracidial formation reduced to 5 and 2.2 % respectively (compared with 60 % for the control group). Following 48 h of exposure of the eggs to seeds extract with 1 mg/mL concentration, the miracidial formation reduced to 0.5 %. In this exposure time, no miracidial formation was observed in the eggs exposed to seeds extract with concentration of 3 mg/mL. Therefore, the results of this study indicated that seeds extract has high ovicidal activity against the eggs of . Accordingly, this extract may have the potential flukicidal activity against the immature and mature.

Scolicidal Effects of Barberry (Berberis vulgaris), Wild Rue Seed (Peganom harmala) and Shirazian Thyme (Zataria multiflora) Extracts on Protoscolices of Hydatid Cysts

“…Biological activity of P. harmala may be due to such components. Antioxidant, antidiabetic, antiviral, and antimicrobial activities of P. harmala are determined in different recent studies (11). Nematollahi and Ghazi (30) reported that P. harmala extract had no significant scolicidal activity that was in accordance with the current study finding.…”

“…However, increasing the exposure time led to higher mortality rate, but the increase was not remarkable (compared with the control group). Recent studies revealed that P. harmala contains bioactive natural components such as catechin, rutin, and p-coumaric acid (11). Biological activity of P. harmala may be due to such components.…”

“…Also, it has dietary and medicinal properties and is used as analgesic and antiseptic in folk medicine (10). Previous studies reported that the extract of P. harmala seeds had antiseptic properties that can kill fungi, bacteria, Plasmodium, and Leishmania (11).…”

Studying of disinvasion action of the disinfectant Virosan for eggs Nematodes genus Trichuris parasitizing in sheep

“…Тому дослідники, проводячи вивчення хімічних та біологічних методів дезінвазії за різних гельмінтозів сільськогосподарських і свійських тварин, використовували її в експериментах (Brewster et al, 2003;Pecson and Nelson, 2005;Voloshyna, 2010;Mun et al, 2012;Katakam et al, 2014). Проте останнім часом окремі автори пропонують використовувати як тест-культури яйця конкретних збудників гельмінтозів, проти яких і ведеться боротьба, оскільки такі дані є більш об'єктивними (Braga et al, 2008;Naidoo et al, 2016;Moazeni, 2017). Проведеними дослідженнями встановлено, що засіб "Віросан" володіє вираженими дезінвазійними властивостями відносно яєць нематод, що підтверджується дослідами інших вчених (Klymenko et al, 2018).…”

Disinvasive efficacy of chlorine-based preparations of domestic production for eggs of nematodes of the species Aonchotheca bovis parasitizing in sheep

“…In order to eliminate and prevent environmental contamination by invasive elements, there is a well-developed health improvement system that combines animal deworming and disinfestation of environmental objects (Naidoo et al, 2016;Moazeni et al, 2017). Experience shows that the main and the most effective among the avail-Citation: Melnychuk, V., & Yuskiv, I.…”