In vitro ovicidal activity of Baccharis conferta Kunth against Haemonchus contortus

Cortes-Morales, Jorge Alberto; Olmedo-Juárez, Agustín; Trejo‐Tapia, Gabriela; González-Cortazar, Manasés; Domínguez-Mendoza, Blanca Eda; Gives, Pedro Mendoza de; Zamilpa, Alejandro

doi:10.1016/j.exppara.2019.01.003

Cited by 38 publications

(22 citation statements)

References 56 publications

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“…The flavonol isokaempferide was recently isolated from a native Mexican plant (Baccharis conferta) and displayed ovicidal effects on H. contortus eggs (IC 50 = 80 µg/mL). From the same plant, the authors also isolated hydroxycinnamic-and 4,5-di-O-caffeoylquinic acid, based on ovicidal effects; however, 100% egg hatching inhibition was only observed at 3 mg/mL [86].…”

Section: Natural Products Active Against Intestinal Parasitic Nematodesmentioning

confidence: 99%

Plant-Based Natural Products for the Discovery and Development of Novel Anthelmintics against Nematodes

2020

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Intestinal parasitic nematodes infect approximately two billion people worldwide. In the absence of vaccines for human intestinal nematodes, control of infections currently relies mainly on chemotherapy, but resistance is an increasing problem. Thus, there is an urgent need for the discovery and development of new anthelmintic drugs, especially ones with novel mechanisms of action. Medicinal plants hold great promise as a source of effective treatments, including anthelmintic therapy. They have been used traditionally for centuries and are mostly safe (if not, their toxicity is well-known). However, in most medicinal plants the compounds active against nematodes have not been identified thus far. The free-living nematode C. elegans was demonstrated to be an excellent model system for the discovery of new anthelmintics and for characterizing their mechanism of action or resistance. The compounds discussed in this review are of botanical origin and were published since 2002. Most of them need further studies of their toxicity, mechanisms and structure-activity relationship to assess more fully their potential as drugs.

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Section: Natural Products Active Against Intestinal Parasitic Nematodesmentioning

confidence: 99%

Plant-Based Natural Products for the Discovery and Development of Novel Anthelmintics against Nematodes

2020

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“…MHz and compound 7 in a Bruker Advance III HD-600 at 600 MHz for 1H NMR, NOESY, 1 H-1 H 222 COSY, HSQC and HMBC, and 150 MHz for 13 C NMR and DEPT in CDCl3 and CD3OD. Chemical 223…”

Section: General Experimental Procedures 219mentioning

confidence: 99%

“…On the other hand, a methanol extract showed ovicidal activity against Haemonchus contortus, where the active compounds were isokaemferide and 4,5-di-O-caffeoylquinic. Phytochemical investigations of B. conferta have reported the isolation of essential oils, diterpene, triterpenes, flavonoids and coumarins [12][13][14][15]. The aim of this study was to determine the anti-inflammatory activity from B. conferta and to identify the compounds responsible for this effect through bioassay-guided fractionation, using the TPA-induced ear edema model in mice.…”

Section: Introductionmentioning

confidence: 99%

Effect of Terpenoids and Flavonoids Isolated from Baccharis conferta Kunth on TPA-Induced Ear Edema in Mice

et al. 2020

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In this study, we isolated from the aerial parts of Baccharis conferta Kunth (i) a new neoclerodane, denominated “bacchofertone”; (ii) four known terpenes: schensianol A, bacchofertin, kingidiol and oleanolic acid; and (iii) two flavonoids: cirsimaritin and hispidulin. All structures were identified by an exhaustive analysis of nuclear magnetic resonance (NMR) and mass spectroscopy (MS). Extracts from aerial parts were screened for anti-inflammatory activity in the mice ear edema model of 12-O-tetradecanoylforbol-13-acetate mice. Dichloromethane extract (BcD) exhibited 78.5 ± 0.72% inhibition of edema, followed by the BcD2 and BcD3 fractions of 71.4% and 82.9% respectively, at a dose of 1 mg/ear. Kingidiol and cirsimaritin were the most potent compounds identified, with a median effective dose of 0.12 and 0.16 mg/ear, respectively. A histological analysis showed that the topical application of TPA promoted intense cell infiltration, and this inflammatory parameter was reduced with the topical application of isolated compounds.

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“…The egg hatching inhibition percentage was calculated by the following formula: [(number of eggs)/(number of larvae + number of eggs) * 100]. 15,16…”

Section: Egg Hatching Inhibition Test (Ehit)mentioning

confidence: 99%

Caesalpinia coriaria fruits and leaves extracts possess in vitro ovicidal activity against Haemonchus contortus and Haemonchus placei

et al. 2019

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The ovicidal effect of hydro-alcoholic extracts from mature fruits and dried leaves of the Caesalpinia coriariatree, against Haemonchus contortus and H. placei was evaluated by the egg hatching inhibition test (EHIT). Secondary compounds within the extracts were identified by high performance liquid chromatography. Five extract concentrations were assessed in the inhibition tests (25.0, 12.5, 6.2, 3.1 and 1.5 mg/mL), using distilled water and Thiabendazole (0.5 mg/mL) as negative and positive controls, respectively. The results were analysed under a 2 × 2 × 6 completely randomized factorial design, and mean differences were established by the Tukey’s post hoc test. The 50% (EC50) and 90% (EC90) effective concentrations for each extract were determined using a Probit analysis.A concentration-dependent ovicidal effect of hydro-alcoholic extracts from both leaves and fruits was observed, reaching a 100% efficacy against both nematode species with the 25.0 mg/mL concentration (p < 0.05). The EC50 for the fruit and leaves extracts were established as 1.63 and 3.91 mg/mL, and as 3.98 and 11.68 mg/mL, against H. contortus and H. placei, respectively. The most important secondary compounds identified in extracts were free-condensed tannins, gallic acid and methyl-gallate.In conclusion,hydro-alcoholic extracts from C. coriaria mature fruits and dried leaves inhibit in vitro egg hatching of H. contortus and H. placei.

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In vitro ovicidal activity of Baccharis conferta Kunth against Haemonchus contortus

Cited by 38 publications

References 56 publications

Plant-Based Natural Products for the Discovery and Development of Novel Anthelmintics against Nematodes

Plant-Based Natural Products for the Discovery and Development of Novel Anthelmintics against Nematodes

Effect of Terpenoids and Flavonoids Isolated from Baccharis conferta Kunth on TPA-Induced Ear Edema in Mice

<em>Caesalpinia coriaria</em> fruits and leaves extracts possess <em>in vitro</em> ovicidal activity against <em>Haemonchus contortus</em> and <em>Haemonchus placei</em>

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