In vitro ovicidal effect of p-coumaric acid from Acacia bilimekii aerial parts against Haemonchus contortus

Cortes-Morales, Jorge Alberto; Zamilpa, Alejandro; Salinas-Sánchez, David Osvaldo; González-Cortazar, Manasés; Tapia‐Maruri, Daniel; Gives, Pedro Mendoza de; Rivas-González, Juan Manuel; Olmedo-Juárez, Agustín

doi:10.1016/j.vetpar.2023.109971

Cited by 9 publications

(5 citation statements)

References 47 publications

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“…These findings suggest that the culture media can influence the production of bioactive mycocompounds derived from the secondary metabolism of fungi. In future studies, we plan to use confocal laser scanning microscopy to perform a co-localization analysis to determine the site of action and the damage caused by metabolites responsible of the nematocidal activity [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

Assessing the In Vitro Individual and Combined Effect of Arthrobotrys oligospora and A. musiformis (Orbiliales) Liquid Culture Filtrates against Infective Larvae of the Sheep Blood-Feeding Nematode Haemonchus contortus (Trichostrongylidae)

Colinas-Picazo,

Mendoza-de Gives,

Pérez-Anzúrez

et al. 2024

Pathogens

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Background: Arthrobotrys species are nematophagous fungi that secrete extracellular nematocidal products (ECP). The individual and combined effects of ECP from Arthrobotrys oligospora (Ao) and A. musiformis (Am) growth in liquid media against Haemonchus contortus L3 (HcL3) were assessed. Methods: The isolation, morphological (MI) and molecular identification (Mol-I), assessment of nematocidal activity (NA) of fungal liquid culture filtrates (LCF) in two liquid media alone and in combination and the myco-compound profile identification (MCP) were performed. Results: The MI suggested that the fungi corresponded to the species Ao and Am. This result was confirmed by PCR analysis followed by sequencing, alignment and a phylogenetic analysis. Likewise, the highest Hc mortalities were 91.4% with individual LCF of Am and 86.2% with those of Ao at the highest concentration (100 mg/mL) in Czapek-Dox Broth. The combination of both LCF resulted in a similarly high larval mortality with no statistical differences in relation to individual activity (p > 0.05). The MCP showed the presence of alkaloids in both fungi. Coumarins, sterols and saponins were found only in Ao. Main conclusions: Both fungi produced ECP with a high NA that could be identified and assessed in future studies as potential natural anthelmintic compounds.

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Section: Resultsmentioning

confidence: 99%

Assessing the In Vitro Individual and Combined Effect of Arthrobotrys oligospora and A. musiformis (Orbiliales) Liquid Culture Filtrates against Infective Larvae of the Sheep Blood-Feeding Nematode Haemonchus contortus (Trichostrongylidae)

Colinas-Picazo,

Mendoza-de Gives,

Pérez-Anzúrez

et al. 2024

Pathogens

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Section: Microscopic Analysis Of Haemonchus Contortus After Exposure ...mentioning

confidence: 99%

Assessing the In Vitro Individual and Combined Effect of Arthrobotrys Oligospora and <em>A. musiformis</em> (Orbiliales) Liquid Culture Filtrates against Infective Larvae of the Sheep Blood-Feeding Nematode <em>Haemonchus contortus</em> (Trichostrongylidae)

Colinas-Picazo,

Mendoza-de Gives,

Pérez-Anzúrez

et al. 2024

Preprint

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The individual and combined effects of extracellular products (ECP) from Arthrobotrys oligospora (Ao) and A. musiformis (Am) growth in liquid media against Haemonchus contortus L3 (HcL3) were assessed. The following methodological steps were established: isolation, morphological (MI) and molecular identification (Mol-I), assessment of nematocidal activity (NA) of fungal liq-uid culture filtrates (LCF) produced in two liquid media alone and in combination and finally, the myco-compound profile identification (MCP). The MI of both isolates suggested that the fungi corresponded to the species Ao and Am. This result was confirmed by PCR analysis followed by sequencing, alignment and obtaining coverage and similarity with respect to isolates reported in the NCBI database. Likewise, the highest Hc mortalities were 91.4% with individual LCF of Am and 86.2% with those of Ao at the highest concentration (100 mg/mL) in Czapek-Dox Broth. The combination of both LCF resulted in a similarly high larval mortality with no statistical differ-ences in relation to individual activity (P>0.05). The MCP showed the presence of alkaloids in both fungi. Coumarins, triterpenes and saponins were found only in Ao. Both fungi produced ECP with a high NA that could be identified and assessed in future studies as potential natural an-thelmintic compounds.

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“…Regarding the appearance of granules in the intestines of H. contortus infective larvae after exposure to the LCF obtained from Cz-DoxB, the authors of the present study do not have a convincing explanation for this fact. The use of confocal laser scanning microscopy analysis may be helpful in demonstrating a co-localisation effect of bioactive compounds into larval tissues to determine the mechanism underlying a possible mode of action [34]. A list of secondary metabolites produced by nematophagous fungi with nematocidal activity against different nematodes, bacteria, and fungi of agriculture and livestock importance is shown in Table 5.…”

Section: Microscopic Analysis Of Haemonchus Contortus Infective Larva...mentioning

confidence: 99%

First Record of Flavocillium subprimulinum (Cordycipitaceae, Hypocreales) in Mexico: Morphological and Molecular Characterisation, Nematocidal Activity of Its Liquid Culture Filtrates against Haemonchus contortus and Protease Activity

Pérez-Anzúrez,

Mendoza-de Gives,

Olmedo-Juárez

et al. 2024

JoF

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This is the first record of the fungus Flavocillium subprimulinum in Mexico. The isolate was taxonomically characterised and cultured in potato dextrose broth (PDB), Czapek–Dox broth (CzDoxB), and sweet potato dextrose broth (SPDB) to obtain its filtrates (FLCF). The nematocidal activity (NA) of three FLCF concentrations was assessed against Haemonchus contortus L3. Protease activity (PA) was assessed with SDS-PAGE, followed by a zymogram. The NA of the FLCF reached 94.43% in PDB and 95.82% in CzDoxB, respectively, at 100 mg/mL. Lower mortality (64%) was found in SPDB at 100 mg/mL. SDS-PAGE showed bands (in PBS) of ~25, ~40, and ~55 kDa. The zymogram showed protein bands (PBs) with PA in the media, including PBs of ~14, ~40, and ~55 kDa. This study establishes the basis for exploring the potential use of this fungus against H. contortus, which is considered the most pathogenic parasite affecting lambs.

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In vitro ovicidal effect of p-coumaric acid from Acacia bilimekii aerial parts against Haemonchus contortus

Cited by 9 publications

References 47 publications

Assessing the In Vitro Individual and Combined Effect of Arthrobotrys oligospora and A. musiformis (Orbiliales) Liquid Culture Filtrates against Infective Larvae of the Sheep Blood-Feeding Nematode Haemonchus contortus (Trichostrongylidae)

Assessing the In Vitro Individual and Combined Effect of Arthrobotrys oligospora and A. musiformis (Orbiliales) Liquid Culture Filtrates against Infective Larvae of the Sheep Blood-Feeding Nematode Haemonchus contortus (Trichostrongylidae)

Assessing the In Vitro Individual and Combined Effect of Arthrobotrys Oligospora and <em>A. musiformis</em> (Orbiliales) Liquid Culture Filtrates against Infective Larvae of the Sheep Blood-Feeding Nematode <em>Haemonchus contortus</em> (Trichostrongylidae)

First Record of Flavocillium subprimulinum (Cordycipitaceae, Hypocreales) in Mexico: Morphological and Molecular Characterisation, Nematocidal Activity of Its Liquid Culture Filtrates against Haemonchus contortus and Protease Activity

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