In vitro performance of an injectable hydrogel/microsphere based immunocyte delivery system for localised anti-tumour activity

Wang, Chunming; Adrianus., Gerard Nathanael; Sheng, Nan; Toh, Shikai; Gong, Yihong; Wang, Dong‐An

doi:10.1016/j.biomaterials.2009.09.006

Cited by 35 publications

(37 citation statements)

References 30 publications

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“…[43] The size range of microspheres fabricated here is also comparable to the range of hydrogel microspheres of natural and synthetic polymers fabricated by different methods including alginate/PLGA-PEG-PLGA composites, [44] PEG-PLGA, [45] alginate-PEG composites, [46] and agarose microspheres. [47] Finally, the gelation chemistry used is versatile, gentle, and adaptable to a number of thiol crosslinkers and PEG macromers. The PEG macromer and thiol crosslinker-based hydrogels can also be rendered biodegradable with tunable degradation rates based on their composition and crosslinking chemistry.…”

Section: Microsphere Diameter Size Distributionmentioning

confidence: 99%

Fabrication of Polyethylene Glycol‐Based Hydrogel Microspheres Through Electrospraying

Jain

Scott

Zustiak

et al. 2015

Macro Materials & Eng

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Polyethylene glycol (PEG) hydrogel microspheres of controlled sizes and narrow size distribution were fabricated via electrospraying. To facilitate the formation of microspheres through electrospraying, the gelation time of PEG hydrogels by Michael's addition reaction between acrylate and thiol were optimized. The effect of electrospraying parameters such as applied voltage, flow rate, needle gauge, and tip to collector distance (TTCD) on Taylor cone formation and microsphere size and distribution was determined. Applied voltage and TTCD had the strongest influence on Taylor cone formation and microsphere size and distribution. By careful design of process parameters a wide range of PEG hydrogel microspheres were obtained, namely 70-700 mm in diameter. The biodegradable PEG hydrogel microspheres developed in this study through a combination of mild gelation chemistry and electrospraying will be valuable in a variety of biological applications including drug and protein delivery, cell encapsulation, and biosensors.

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Section: Microsphere Diameter Size Distributionmentioning

confidence: 99%

Fabrication of Polyethylene Glycol‐Based Hydrogel Microspheres Through Electrospraying

Jain

Scott

Zustiak

et al. 2015

Macro Materials & Eng

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“…Chondrocyte viability was determined qualitatively and quantitatively (Wang et al ., ). For Live/Dead staining (Molecular probes, Invitrogen), calcein (0.5 μl/ml) and ethidium homodimer (2 μl/ml) were added to culture medium containing the LhCG construct and incubated at 37°C for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…Chondrocyte viability was determined qualitatively and quantitatively (Wang et al, 2009). For Live/Dead staining (Molecular probes, Invitrogen), calcein (0.5 μl/ml) and ethidium homodimer (2 μl/ml) were added to culture medium containing the LhCG construct and incubated at The steps for setting up the tri-culture system, which involve: macrophage differentiation and seeding of SW982 cells on the day before (denoted as Day -0), as well as LPS activation of macrophages on Day 0.…”

Section: Live/dead Staining and Wst-1 Cell Viability Assaymentioning

confidence: 99%

Establishment of anin vitrothree-dimensional model for cartilage damage in rheumatoid arthritis

Peck

Leom

Low

et al. 2017

J Tissue Eng Regen Med

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Rheumatoid arthritis (RA) is a chronic inflammatory disease that leads to progressive joint destruction. To further understand the process of rheumatoid cartilage damage, an in vitro model consisting of an interactive tri-culture of synovial fibroblasts (SFs), LPS-stimulated macrophages and a primary chondrocyte-based tissue-engineered construct was established. The tissueengineered construct has a composition similar to that of human cartilage, which is rich in collagen type II and proteoglycans. Data generated from this model revealed that healthy chondrocytes were activated in the presence of SFs and macrophages. The activated chondrocytes subsequently displayed aberrant behaviours as seen in a disease state such as increased apoptosis, decreased gene expression for matrix components such as type II collagen and aggrecan, increased gene expression for tissuedegrading enzymes (MMP-1, -3, -13 and ADAMTS-4, -5), and upregulation of inflammatory mediator gene expression (TNF-α, IL-1β, IL-6 and IKBKB). Additionally, the inclusion of SFs and macrophages in the model enabled both cell types to more closely replicate an in vivo role in mediating cartilage destruction. This is evidenced by extensive matrix loss, detected in the model through immunostaining and biochemical analysis. Subsequent drug treatment with celecoxib has shown that the model was able to respond to the therapeutic effects of this drug by reversing cartilage damage. This study showed that the model was able to recapitulate certain pathological features of an RA cartilage. If properly validated, this model potentially can be used for screening new therapeutic drugs and strategies, thereby contributing to the improvement of anti-rheumatic treatment.

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“…Therapeutic cell transplantation, which has drawn increasing attention and has become more and more important in regenerative medicine in the recent decade, requires that the cell-laden vehicles with conveyable and vast cell-loading interfaces can be injected into the targeted site in vivo and degrade after therapy (1)(2)(3)(4). Since anchorage-dependent cells (ADCs) are a major family of therapeutic cell species, the cell microcarriers also need appropriate surface characteristic to support cell proliferation and ensure intact cell phenotype during the delivery (5)(6)(7).…”

Section: Introductionmentioning

confidence: 99%

A Novel Shell-Structure Cell Microcarrier (SSCM) for Cell Transplantation and Bone Regeneration Medicine

Gong

Wang

et al. 2010

Pharm Res

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In vitro performance of an injectable hydrogel/microsphere based immunocyte delivery system for localised anti-tumour activity

Cited by 35 publications

References 30 publications

Fabrication of Polyethylene Glycol‐Based Hydrogel Microspheres Through Electrospraying

Fabrication of Polyethylene Glycol‐Based Hydrogel Microspheres Through Electrospraying

Establishment of anin vitrothree-dimensional model for cartilage damage in rheumatoid arthritis

A Novel Shell-Structure Cell Microcarrier (SSCM) for Cell Transplantation and Bone Regeneration Medicine

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