In Vitro Pharmacological Study of Extract of Raphanus Sativus Sprouts

Abstract

Ethyl acetate extract of Raphanus sativus sprouts belonging to Brassicaceae, produced by cold maceration reveal the presence of volatile oils. Dilutions of the extract 20, 40, 60, 80, 100 µg/ml prepared in DMSO were used for the in vitro study. The antimicrobial activity of the extract was assessed using Bacillus subtilis (NCIM 2698), Staphylococcus aureus (NCIM 2079), Escherichia coli (NCIM 2344), Klebsiella pneumonia (NCIM 2957) and Aspergillus flavus (NCIM 568) against the standards gentamycin and ketoconazole. In vitro pharmacological studies, include the reducing activity, antioxidant activity and anti inflammatory activity were evaluated using the ferric reducing assay, hydrogen peroxide scavenging assay and albumin denaturation method respectively. The extract had shown significant antibacterial activity on all organisms excluding Klebsiella and Escherichia than the standard. In vitro studies reveal significant reducing (36.015 + 0.686), antioxidant (26.830 + 0.240) and anti inflammatory activities (25.865 + 0.191) against the standard butylated hydroxyl toluene (BHT) (31.879 + 2.629), ascorbic acid (21.7245 + 0.240) and Diclofenac (0.4825 + 0.025) respectively.

LC-MS- and 1H NMR-Based Metabolomics to Highlight the Impact of Extraction Solvents on Chemical Profile and Antioxidant Activity of Daikon Sprouts (Raphanus sativus L.)

“…The DPPH assay highlighted the highest scavenging activity for the 70% EtOH/H 2 O extract (IC50 93.97 µg/mL) ( Table S1 ). In the literature, the antioxidant activity was reported for MeOH, CHCl 3 and CH 3 COCH 3 extracts of daikon sprouts [ 6 , 13 ]. Herein, the antioxidant activity exhibited by the “green” extracts represents the possibility to use these extracts as a nutraceutical ingredient.…”

“…Isolated compounds were characterized by NMR analysis. The antioxidant activity related to daikon sprouts was previously evaluated for MeOH, CHCl 3 and CH 3 COCH 3 extracts [ 6 , 13 ]. Herein, the radical scavenging activity of the MeOH extract and “green” extracts obtained by using EtOH and EtOH:H 2 O (50% and 70% v / v ) mixtures was determined by 1-diphenyl-2-picrylhydrazyl (DPPH) and Trolox Equivalent Antioxidant Capacity (TEAC) assays.…”