2012
DOI: 10.1371/journal.pone.0032109
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In Vitro Phenotypic, Genomic and Proteomic Characterization of a Cytokine-Resistant Murine β-TC3 Cell Line

Abstract: Type 1 diabetes mellitus (T1DM) is caused by the selective destruction of insulin-producing β-cells. This process is mediated by cells of the immune system through release of nitric oxide, free radicals and pro-inflammatory cytokines, which induce a complex network of intracellular signalling cascades, eventually affecting the expression of genes involved in β-cell survival.The aim of our study was to investigate possible mechanisms of resistance to cytokine-induced β-cell death. To this purpose, we created a … Show more

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Cited by 16 publications
(13 citation statements)
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“…An independent analysis of DEPs-one step interacting proteins relations was performed. SUMO4 as a one-step interacting protein was reported to resistant with Type 1 diabetes mellitus 57 , which was referred to five DEPs (GSTP1, G6PD, IDH1, ALDOA, LDHB) simultaneously. These five proteins had been proved to be correlated with resistant drug directly or indirectly by HPRD.…”
Section: Discussionmentioning
confidence: 99%
“…An independent analysis of DEPs-one step interacting proteins relations was performed. SUMO4 as a one-step interacting protein was reported to resistant with Type 1 diabetes mellitus 57 , which was referred to five DEPs (GSTP1, G6PD, IDH1, ALDOA, LDHB) simultaneously. These five proteins had been proved to be correlated with resistant drug directly or indirectly by HPRD.…”
Section: Discussionmentioning
confidence: 99%
“…Disorders of immune homeostasis induce a complex network of intracellular signaling cascades to induce T1DM 6 , including the Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathways. In animal models, neutralization of JAK2-STAT3 signaling appears to protect against T1DM 7 .…”
mentioning
confidence: 99%
“…Spots of interest were manually picked and mass spectrometric sequencing was carried out after in-gel digestion, using sequencing-grade trypsin (20 µg/vial), as previously described [18]. The tryptic peptide extracts were dried in a vacuum centrifuge and dissolved in 0.1% trifluoroacetic acid (TFA).…”
Section: In-gel Digestion and Ms Aanalysis Of Tryptic Digestsmentioning
confidence: 99%