In vitro photodynamic inactivation of Candida albicans by phenothiazine dye (new methylene blue) and Indocyanine green (EmunDo®)

Fekrazad, Reza; Barghi, Vadood Ghasemi; Mir, Arash Poorsattar Bejeh; Shams-Ghahfarokhi, Masoomeh

doi:10.1016/j.pdpdt.2014.12.006

Cited by 35 publications

(18 citation statements)

References 33 publications

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“…Candida albicans (C. albicans, ATCC 60193) was incubated on Sabouraud Dextrose Aga (SDA) for 24 h at 30 • C. Then, it was transferred to Sabouraud Dextrose broth medium and incubated overnight at 37 • C with shaking (180 rpm) and diluted with sterile distilled water to 3 × 10 5 CFU/mL, which was counted by hemocytometer [26].…”

Section: Inoculum Preparationmentioning

confidence: 99%

Dermal Targeting Delivery of Terbinafine Hydrochloride Using Novel Multi-Ethosomes: A New Approach to Fungal Infection Treatment

Zhang

Zhu

et al. 2020

Coatings

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This research aimed to develop and evaluate a novel multi-ethosome (ME) system for the dermal delivery of terbinafine hydrochloride (TH) as a new approach to fungal infection treatment. TH-loaded MEs were successfully prepared using cinnamaldehyde as a penetration enhancer. Mean diameter of ME was found as ~100 nm with monodispersed size distribution. Drug entrapment efficiency reached up to 86% ± 1.4%. MEs exhibited excellent colloid stability and no drug leakage after 2 months of storage. In contrast to a commercial Lamisil® cream, ME significantly improved the targeting efficiency by increasing the fluidity of stratum corneum layer, revealed by attenuated total reflection Fourier transformed infrared spectroscopy (ATR-FTIR). The dermal targeting effect was visualized using confocal microscopy. Moreover, skin irritation and allergy tests showed that ME was not irritating to the skin. The improved antifungal activity of ME was proved in vitro on Candida albicans strains by minimal inhibitory concentration (MIC) assay. This study paves the way towards design of MEs for dermal fungal infection treatment.

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Section: Inoculum Preparationmentioning

confidence: 99%

Dermal Targeting Delivery of Terbinafine Hydrochloride Using Novel Multi-Ethosomes: A New Approach to Fungal Infection Treatment

Zhang

Zhu

et al. 2020

Coatings

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Section: Discussionmentioning

confidence: 99%

“…To date, several in vitro studies have been published on the successful application of ICG‐mediated PACT for the eradication of microorganisms 44,48‐51 . Laser application plus ICG caused a significant reduction in Candida albicans CFUs compared with the control group 50,51 . The bactericidal effects of ICG‐PDT and near‐infrared light against Staphylococcus aureus , Streptococcus pyogenes , and Pseudomonas aeruginosa were also investigated and all species found to be susceptible to killing by this modality 44 .…”

Section: Discussionmentioning

confidence: 99%

Photodynamic antimicrobial chemotherapy using indocyanine green in experimentally induced intraoral ulcers in rats

Motamedifar

Tanideh

Mardani

et al. 2020

Photoderm Photoimm Photomed

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Background Photodynamic antimicrobial chemotherapy (PACT) is a promising modality for eradication of microorganisms from the wound. This study aimed to investigate the effectiveness of PACT using indocyanine green (ICG) for reduction of bacterial load of oral ulcers in rats and its impact on the healing process. Methods In this experimental study, 50 adult male Sprague Dawley rats were recruited. Oral ulcers were surgically made on the left cheek mucosa, and animals were randomly assigned into five groups (n = 10). Wound site in groups 1, 2, and 3 was irrigated with the sterile saline (0.9%), chlorhexidine (CHX; 0.2%), and ICG solutions (1 mg/mL), respectively. Group 4 was exposed to laser irradiation using 810 nm diode laser on continuous‐wave mode for 30 seconds (fluence: 55 J/cm2, power: 300 mW, spot size: 4.5 mm). In group 5, PACT was performed using topical application of ICG followed by laser irradiation in the same way as the previous group. Bacterial load of oral ulcers was assessed before and after each treatment modality. Besides, rats were sacrificed on the 5th day post ulceration and histological features of healing were evaluated. Results Bacterial load was significantly reduced merely in the PACT‐ICG‐treated group by one log (P < .0001). Animals in the PACT‐ICG‐treated group also showed an accelerated healing in comparison with others on the 5th day of an experiment. Conclusion Photodynamic antimicrobial chemotherapy using topical application of ICG has a potential to reduce the bacterial load of oral ulcers and accelerate wound repair. Therefore, it can be considered as an alternative to currently available modalities for wound management.

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“…On the other hand, 58 excited with lower wavelengths works as a typical PS in PDT. Fekrazad et al reported activity of 58 in decreasing growth rate of C. albicans and T. rubrum [74,85,248]. Nevertheless, the reduction was found out at the level of ca.…”

Section: Other Photosensitizersmentioning

confidence: 98%

Photosensitizers Mediated Photodynamic Inactivation against Fungi

Ziental

Mlynarczyk

Czarczyńska-Goślińska

et al. 2021

Nanomaterials

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Superficial and systemic fungal infections are essential problems for the modern health care system. One of the challenges is the growing resistance of fungi to classic antifungals and the constantly increasing cost of therapy. These factors force the scientific world to intensify the search for alternative and more effective methods of treatment. This paper presents an overview of new fungal inactivation methods using Photodynamic Antimicrobial Chemotherapy (PACT). The results of research on compounds from the groups of phenothiazines, xanthanes, porphyrins, chlorins, porphyrazines, and phthalocyanines are presented. An intensive search for a photosensitizer with excellent properties is currently underway. The formulation based on the existing ones is also developed by combining them with nanoparticles and common antifungal therapy. Numerous studies indicate that fungi do not form any specific defense mechanism against PACT, which deems it a promising therapeutic alternative.

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In vitro photodynamic inactivation of Candida albicans by phenothiazine dye (new methylene blue) and Indocyanine green (EmunDo®)

Cited by 35 publications

References 33 publications

Dermal Targeting Delivery of Terbinafine Hydrochloride Using Novel Multi-Ethosomes: A New Approach to Fungal Infection Treatment

Dermal Targeting Delivery of Terbinafine Hydrochloride Using Novel Multi-Ethosomes: A New Approach to Fungal Infection Treatment

Photodynamic antimicrobial chemotherapy using indocyanine green in experimentally induced intraoral ulcers in rats

Photosensitizers Mediated Photodynamic Inactivation against Fungi

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