In Vitro Planlet Induction of Tropical Pitcher Plant (Nepenthes Ampullaria Jack) by Various Thiamin and Benzyl Amino Purine Concentrate

Abstract

Nepenthes is one of Indonesian tropical plant as a biodiversity source that endangered from its extinction. One of the effort to prevent its extinction by using plant tissue isolation method on Nepenthes multiplication are needed. This research was aimed to determine the best interaction between Thiamin and benzyl amino purine concentration on in vitro Nepenthes planlet growth, Thiamin best concentration on in vitro Nepenthes planlet growth, and benzyl amino purine best concentration on in vitro Nepenthes planlet growth. The research was conducted in Agriculture Department Biotechnology Laboratory Universitas Pembangunan Nasional “Veteran” Yogyakarta on January – April 2018. Completed Random Design method with 2 factors was used in this research. The first factor was various concentration of Thiamin with 8 ppm, 10 ppm, and 12 ppm. The second factors was various concentration of benzyl amino purine with 0,5 ppm, 1 ppm, and 1,5 ppm. Every combination was repeated 3 times. The result of this research showed that no interaction was found in every Thiamin and benzyl amino purine various concentration on in vitro Nepenthes planlet. The usage of 10 ppm Thiamin concentration (T2) produce the best results on sprout’s amount and sprout’s height. On 1 ppm of benzyl amino purine concentration produce the best result on sprout’s amount, sptout’s height, and amount of leaves.Keyword: Nepenthes, Thiamin, Benzyl Amino Purine

In Vitro Multiplication of Nepenthes mirabilis Lour (Druce) with Variations Concentration of Sucrose and BAP

“…Sucrose as a carbon source plays a role in cell division, cell enlargement, and cell differentiation (Baskara et al, 2018). Environmental conditions such as temperature, humidity, light and oxygen are other supporting factors (Cryssanti et al, 2019). Purba (2021) stated that without the addition of BAP in the medium, explants can live well.…”

“…Triyastuti et al (2018) stated that the concentration of 20 g/l was the optimal concentration for producing the highest number of leaves. Cryssanti et al (2019) reported that low concentrations of BAP were able to produce a large number of leaves. Previaningrum et al (2021) found that the use of PGR in low concentrations showed an increase in the number of leaves.…”