In vitro plant propagation and crop improvement in Lisianthus (Lisianthus Russelianus Hook.)

Abstract

Romania assists at the present time to an increase of production crops for ornamental plants and as a consequence an increased demand of planting material. Thus, improvements of the current multiplication methods are sought after. Lisianthus russelianus Hook. (Eustoma grandiflorum Grise.) is a relatively new floral crop to the international market, known for beautiful flowers of various colors and for having a long vase life. This study focused on the development of an efficient protocol for rapid regeneration of this species following known basic and applied aspects of lisianthus biotechnology but exploring new potentials. In the course of experiments conducted, for in vitro multiplication there were used nodal segments (1.5 cm) with axillary buds from three F 1 hybrids 'Echo Lavender', 'Flamenco White', 'Mirage Pastel Pink' that were inoculated on MS basal medium supplemented with 0.50 mg 1 -1 TDZ, 1.0 mg 1 -1 BAP and 0.50 mg 1 -1 AIA. The results show that the medium with BAP was most effective for obtaining the highest shoots number compared to medium containing TDZ. For rooting induction, two different concentrations of auxin IBA 0.5 mg 1 -1 and 1.5 mg 1 -1 were used simultaneously on MS basal medium. The highest roots number occurred when using 1.5 mg 1 -1 IBA. Both the number of shoots and rooting regeneration were dependent on the cultivar. The highest shoots number was achieved for 'Mirage Pastel Pink' hybrid (6.91) on the medium containing 1.0 mg 1 -1 BAP and 0.50 mg 1 -1 IAA.

Micropropagation of Lisianthus [Eustoma grandiflorum (Raf.) Shinn.] Leaf Explants and Single Nodes in Response to Plant Growth Regulators

“…Therefore, the use of BAP alone was determined to be preferable for shoot regeneration and propagation from leaf explants. It can be observed from the literature that different types and doses of growth regulators are used for in vitro somatic embryogenesis or organogenesis in lisianthus (Kaviani, 2014;Pop et al, 2016;Yumbla-Orbes et al, 2020).…”

“…In our study, single node explants prepared from in vitro regenerated shoots were used in the sub-culturing stage and new axillary shoot development per explant could be achieved up to 15.2 shoots in the most suitable medium composition. Pop et al (2016), successfully obtained axillary shoots from single node cultures. The low doses of PGR used in their study, resulted in a limited number of axillary shoots obtained.…”