In vitro plant regeneration derived from leaf and stem explants of endemic Thermopsis turcica

Abstract

This plant tissue study of micropropagation identifies the selective medium saving for rapid propagation in cultivated Thermopsis turcica, an endangered germplasm of the family Fabaceae. The aim is to obtain the optimum growth medium of T. turcica by enabling the in vitro propagation of this endemic. In this study, the leaves and stems of T. turcica were cultured on a Murashige and Skoog's medium supplemented with various concentrations (0.5, 1.0 and 2.0 mg L −1 1-Naphthaleneacetic acid) of auxin and (0.2 and 0.5 mg L −1 Zeatin) (1.0 and 2.0 mg L −1 Benzylaminopurine) of cytokinins. Previous research focused on the regeneration from the seed of T. turcica Eber population; we concentrated upon the regeneration of different plant parts (leaf and stem) of T. turcica Aksehir population. In addition, according to the literature on T. turcica that to date the effects of Zeatin on the regeneration has not been performed. The most promising regeneration and growth were obtained from leaf explants cultured on the media with 2.0 mg L −1 1-Naphthaleneacetic acid and 0.5 mg L −1 Zeatin (93.3%). The regenerated plantles were rooted on the media containing 2.0 mg L −1 Indole-3-butyric acid. Rooted plantlets were transplanted into potting of sterilized soil. The present study reports on the sufficient in vitro regeneration protocol through organogenesis in T. turcica. The findings presented here have implications for in vitro protection and use of this endemic endangered species in further biotechnological research.

Simplified in vitro propagation protocol for Tacca leontopetaloides (L.) Kuntze and assessment of genetic uniformity of regenerated plantlets

“…Later on, a whitish green compact callus developed in these treatments. Comparable to our study, in many species from various botanical families, e.g., Drosera intermedia (Rejthar et al, 2014), or Thermopsis turcica (Tekdal and Cetiner, 2014), in vitro propagation using zeatin led to improvements of previously published protocols, based on the application of other cytokinins (Cenkci et al, 2009;Grevenstuk et al, 2010). On the other hand, for numerous species from the Dioscoreaceae family, BA still remains sufficiently effective for in vitro propagation, e.g., for T. chantrieri (Charoensub et al, 2008), Dioscorea multiflora (de Souza et al, 2011), D. zingiberensis (Chen et al, 2003), and.…”

In vitro Regeneration Studies of Vuralia turcica Using Unpollinated Ovaries

“…(Chand & Sahrawat 2007), Azadirachta indica (Srivastava et al 2009), Amorphophallus konjac (Zhao et al 2012). However, few reports on in vitro embryogenesis in Vuralia turcica has been reported from leaves, stems (Tekdal & Cetiner 2014a), and mature embryos (Tekdal & Cetiner 2014b). In the present study, the effects of culture conditions on the development of somatic embryos from cultured unpollinated ovaries of V. turcica were investigated for the first time, according to a literature review.…”