In vitro plant regeneration from hypocotyl of Arben (Rubus fraxinifolius Poir.)

Abstract

Rubus fraxinifolius, a group of wild raspberry, was improved as a fresh fruit in Indonesia. The purpose of this study was to develop a protocol for micropropagation of R. fraxinifolius. Cultures were subcultured from hypocotyls (1 cm) which is initiated from seeds. The experiments were focused on the effect of plant growth regulators concentrations and combinations. The highest germination by in vitro was obtained using MS medium supplemented with 10 mg/L of GA 3. Furthermore, based on five parameters, i.e. callus height, callus diameter, number of leaves, plantlet height and number of roots, medium 3 (0.5 mg/L GA 3 , 2.5 mg/L 2-iP and 0.1 mg/L NAA) shown the best results during the development explants of R. fraxinifolius. Effectivity of R. fraxinifolius regeneration through in vitro was affected by 2-iP and NAA concentration. If both of plant growth regulators were high (medium 8) or low (medium 1), it made less growth.

Micropropagation of Three Endemic Begonias Using Various Hormones Concentration and Culture Media Application

“…Sterilization of explants was conducted following sterilization methods by Ismaini et al (2017). The fruit explants were cut transversely in a petri dish throughout the planting procedure, and the seeds were planted into each MS media without growth regulators.…”