In vitro plant regeneration of 4 Capsicum spp. genotypes using different explant types

Orlinska, M; Nowaczyk, P.

doi:10.3906/biy-1403-89

Cited by 24 publications

(28 citation statements)

References 25 publications

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“…Hormones are mostly used after seedling is developed also in MS basal culture medium, not intending to reduce growth time, but to induce multiple shoot bud, callus formation, plant regeneration, flower induction, fruit development and rooting. Considerations for seed viability in germination rate or even seeds pretreatment, which can trigger or enhance germination, were not still reported (Bora et al, 2014;Manzur et al, 2013;Orlinska & Nowaczyk, 2015;Verma et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…These studies have resulted in different protocols and observed a significant diversity of morphogenetic responses dependent on genotype, the explant type and the culture medium conditions (Akram, Gaafar, Safwat, & Diab, 2011;Bora et al, 2014;Gogoi, Acharjee, & Devi, 2014;Grozeva, Rodeva, & Kintzios, 2009;Manzur et al, 2013;Orlinska & Nowaczyk, 2015;Raj, Glint, & Babu, 2015;Valadez-Bustos et al, 2009;Verma, Dhiman, & Srivastava, 2013). Among the various types of explants for the initial establishment of in vitro cultures, the seeds have advantages such as rapid growth, rapid availability of material for subsequent cultivation stages.…”

Section: Discussionmentioning

confidence: 99%

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Optimizing in vitro germination of Capsicum baccatum L. seeds through a multifactorial experimental design

Fidemann¹,

Nascimento²,

Moraes³

et al. 2016

AIJB

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The Capsicum genus comprises hundreds of varieties with fruits that differ widely in shape, size, color, and flavor. The Brazilian cuisine consumes Capsicum baccatum L. (pepper) extensively and few reports about chemical composition and biological properties about this pepper were published. The present study determined four factors numeric effects on germination in vitro of this specie, among them, the solution type for osmotic conditioning (water and 1% KNO3 aqueous solution), germination medium (agar and agar + gibberellic acid at 1.88 mgL -1 ), post-seeding time (15 and 30 days) and two genotypes (Pitanga and Cambuci) on two germination parameters (germination and cotyledon emerging rates), as first step for establishing a cell suspension culture to produce secondary metabolites. The methodology was a two-level full factorial experimental design (2 4 ). The results allowed polynomial equations definition which describes the germination phenomena as a function of the four factors under study. The genotype responses to osmotic conditioning and germination medium were different. The optimal combination of treatments for in vitro germination and development of seedlings for Pitanga and Cambuci was water + agar and water + agar-GA3, respectively.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Optimizing in vitro germination of Capsicum baccatum L. seeds through a multifactorial experimental design

Fidemann¹,

Nascimento²,

Moraes³

et al. 2016

AIJB

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“…Among the overexpression transformants, many showed an altered fruit colour phenotype, varying from the red of the parental Balady and to bright orange of Topepo rosso. The low efficiency of plant regeneration and transformation by pepper cultures has been widely reported (Steinitz et al, 1999;Li et al, 2003;Sharma et al, 2006;El Nagar 2012;Verma et al, 2013;Orlinska and Nowaczyk, 2015).…”

Section: In Vitro Regeneration and Genetic Transformation Of Peppermentioning

confidence: 99%

Genetic engineering to improve β-carotene content in pepper

Nagar

Mokhtar²

2018

Annals of Agricultural Science, Moshtohor

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A laboratory study was conducted to develop a broadly applicable in vitro regeneration and transformation methods for increasing β-carotene content in pepper fruits. Therefore, two different pepper genotypes were analyzed with regard to their efficiency for regeneration and genetic transformation in vitro. Cotyledons were used in vitro from young seedlings as target tissue for regeneration and transformation of pepper. Over expression of β-Lcy gene via Agrobacterium-mediated transformation was used. The presence of the transgene was assayed on leaf DNA via PCR and biochemical analysis using HPLC. Results strongly showed that there were significant differences between pepper genotypes. The transgenic pepper plants showed a significant increase in fruit β-carotene content reaching 7 to 10 folds according to the genotype. The fruits from these plants displayed different colour phenotypes, from orange to orange-red. The difference in the fruit colour phenotypes reflects the beta-carotene ratio, which ranges from 4.5 mg/100g dw in the wild plants to 45.0 in the transgenic plants of genotype Balady during maturity stage. Concerning to the genotype Topepo rosso, the β-carotene content was increased from 5.2 mg/100g dw in the wild plants to 36.39 mg/100g dw in the transgenic plants during mature stage.

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“…It is the world's most important vegetable after tomato and used as fresh, dried or processed products, as vegetables and spices or condiments (Acquaah, 2004). Its nutritional properties including antioxidants are important for human nutrition (Mateos et al, 2003;Orlinska and Nowaczyk, 2015), phenolics "flavonoids" (Bae et al, 2012), carotenoids (Ha et al, 2007), vitamin C, vitamin E (Garcia-Closas et al, 2004) and alkaloids (Srinivas et al, 2009), which play an important roles in human health. Moreover, pepper is also a source for a natural colors and as medicine (Valadez-Bustos et al, 2009 andZhuang, et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

GENOTYPIC AND PHENOTYPIC PATH ANALYSIS STUDIES ON CHILLI PEPPER (Capiscum annuum L.)

2018

Journal of Productivity and Development

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ABSTARACTThe present study were conducted at Privet Farm in Al-Rahmania Island, Behaira Governorate, Egypt, during two successive summer seasons of 2016 and 2017, to identify the interrelationships among fruit yield and its related characters using correlation and improved model of path analysis at both genotypic and phenotypic levels on chilli pepper (Capsicum annuum L.).The experimental genotypes were grown in randomized complete blocks design (RCBD), with three replicates on open field. Eight tested genotypes namely, i. e. Shata Balady Hot cv, Shata Balady Hot line-1, Shata Balady Sweet cv, Serrano cv, Shata Balady Sweet line-3, Shata Balady Sweet line-4, Anheium cv, and Cayenne Large cv were used in this study. The characteristics were studied i.e., plant height (PH); branches number plant -1 (BN); fruit length (FL); fruit diameter (FD); locales number fruit -1 (LN); average fruit weight fruit -1 (AFW); fruits number plant -1 (FN) and yield plant -1 (Y).The results revealed that, there are a significant differences among genotypes for all studied characters. Also, significant and positive correlation coefficients were obtained between fruit yield plant -1 and number of fruits plant -1 , at the genotypic and phenotypic levels (0.743 * and 0.742 * ), respectively. Moreover, significant and negative correlation coefficients were obtained between yield plant -1 and fruit diameter (-0.889 ** and -0.882 ** ) at the genotypic and phenotypic levels, respectively.Path analysis (genotypic and phenotypic) coefficient showed that the traits, i.e. branches number plant -1 and fruits number plant -1 and the average of fruit weight gave the maximum influence directly and indirectly upon yield plant -1 in chilli pepper indicating their magnitude as selection criteria to obtain a valuable gain of selection for yield in chilli pepper. 388 BADR & GENDYConclusively, these results may be helpful to plan appropriate selection strategies for improving fruit yield in hot pepper.

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In vitro plant regeneration of 4 Capsicum spp. genotypes using different explant types

Cited by 24 publications

References 25 publications

Optimizing in vitro germination of Capsicum baccatum L. seeds through a multifactorial experimental design

Optimizing in vitro germination of Capsicum baccatum L. seeds through a multifactorial experimental design

Genetic engineering to improve β-carotene content in pepper

GENOTYPIC AND PHENOTYPIC PATH ANALYSIS STUDIES ON CHILLI PEPPER (Capiscum annuum L.)

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