In Vitro Plant Regeneration of Andrographis Paniculata Nees Using Mature Zygotic Embryonic Explants

Abstract

A rapid method for the large-scale propagation of Andrographis paniculata through in vitro culture of embryonic explants has been developed. Adventitious shoot regeneration from embryonic explants was possible in case of cotyledons and root decapitated embryonic axis among all the tested embryonic explants. High frequency of adventitious shoot formation (?) was observed within 30 days from embryonic explants and shoot induction followed either direct regeneration on MS medium supplemented with BAP or indirect adventitious shoot regeneration in TDZ. BAP was more effective than other cytokinins used for direct adventitious shoot induction from both the embryonic explants of A. panicualata with BAP 1.5mg/L producing 73% shooting and 12.3 shoots per explants while study in cotyledons showed best response in BAP 3.0 mg/L with 52% shooting and 9.3 shoots per explants. While TDZ 1.0mg was effective in indirect shoot regeneration among the different concentrations tested with 26% shoot regeneration from embryonic axis with 3.3 shoots and 76% from cotyledons with 6 shoots per explants. Shoots elongated in MS media with GA 0.5mg/L are effectively rooted in half strength MS media with IBA 0.5mg/L and a high percentage of (82.5%) plants were successfully hardened in soil, sand and vermiculite (1:1:1).