In vitro plant regeneration of caper (Capparis spinosa L.) from floral explants and genetic stability of regenerants

Abstract

A new technique to regenerate caper plants (Capparis spinosa L. subsp. rupestris) starting from flower explant is reported. In vitro plant regeneration was attempted using stigma, anthers and unfertilized ovules of unopened flowers collected in the field. Plant regeneration was achieved from unfertilized ovules on MS medium supplemented with 88 mM sucrose and 13 lM 6-benzyladenine (BA). New individuals obtained from unfertilized ovules were used as source material for micropropagation and multiple shoots were obtained on MS medium sup- plemented with the adeninic cytokinin BA and the auxin indole-3-butyric acid (IBA). Explants obtained in micro- propagation step were used for rooting step under several treatments. The best results (100% of rooted explants) were obtained when explants were dipped for 10 min in 50 lM IBA solution and successively maintained in growth regulator free medium. New plants were vigorous, of good quality and presented phenotypic characters similar to mother plants. Furthermore genetic stability of regenerants was verified through flow cytometric analysis and two different DNA-based techniques

Improved walnut mass micropropagation through the combined use of phloroglucinol and FeEDDHA

“…Somaclonal variation cannot be excluded, however, unless whole genomes are analyzed. In any case, the fact that our protocol does not involve a callus phase should significantly reduce this possibility (Carra et al 2012). …”

Genetically stable plants with boosted flavonoids content after in vitro regeneration of the endangered Capparis spinosa L

“…spinosa L. subsp. Rupestris in Italy from flowerexplant with 13 µM BAP [8]. The main objective of this study was to develop a protocol for an in vitro micropropagation; using shoot tip explant and a regeneration system; using leaf, root or stem segments of C. spinosa L. from North Sinai with a stablegenetic profile and better bioactive flavonoids yield towards the conservation of this medicinally active species.…”

Analysis of genetic and epigenetic variation in in vitro propagated potato somatic hybrid by AFLP and MSAP marker

“…In spite of this limitation, the MSAP method has been successfully applied in a wide range of studies where alterations in cytosine methylation were detected in various crop species (Bednarek et al 2007). The AFLP and the MSAP markers have been used to detect significant genetic and epigenetic changes, respectively in a number of crop species for example potato (Joyce and Cassells, 2002;Dann and Wilson, 2011), Solanum aculeatissimum (Ghimire et al 2012), Triticum aestivum (Meng et al 2012), Gardenia jasminoides (Wu et al 2012), Ungernia victoris (Bublyk et al 2012), Capparis spinosa (Carra et al 2012), Phaseolus ssp. (Abid et al 2011), Nicotiana tabacum (Yang et al 2011), Freesia hybrida (Gao et al 2010), Ocotea catharinensis (Hanai et al 2010), Cymbidium (Chen et al 2009), Vitis spp.…”