In vitro Plantlet Regeneration from Callus Culture of Trachyspermum copticum

Abstract

The effect of explant sources and plant growth regulators on callus induction and plantlet regeneration of Trachyspermum copticum were explored. Different explants including hypocotyl, cotyledonary node and leaf were cultured on MS supplemented with different combinations and concentrations of plant growth regulators including 2,4-D (0.2-3 0.5 mg/l), NAA (2 mg/l), BAP (1-3 mg/l), Kn (0.5 mg/l) and IAA (0.8 mg/l). The best response for callus induction (100%) as well as quality was observed from cotyldonary node segments cultured on MS supplemented with 2, 4-D at 1 mg/l in combination with Kn at 0.5 mg/l. Calli derived from various explants were subcultured on shoot induction media with different compositions and concentrations of medium. MS without any plant growth regulator promoted the highest frequency of shoot regeneration (100%) and also mean number of developed shoots per explants (3.8) showed the same result. Regenerated shoots were then rooted on three-fourth strength MS with 75% efficiency after 30 days.

In vitro axillary shoot regeneration from cotyledonary node of Bishop’s weed (Trachyspermum ammi L.)

“…On the other hand, callus induction frequency (p<0.01), shoot counts (p<0.05) and shoot length (p<0.01) were statistically significant to different concentration of TDZ-IBA. The nature/type of explant is highly significant for in vitro shoot induction and proliferation and cotyledonary node explant is very potent explant reported for various important crops (Chaudhary et al, 2007;Kendir et al, 2008;Teymourian et al, 2017). The results revealed the supermacy of cotyledonary node explant and recorded 100.00%.…”

“…The previous study on T. ammi using seed explant cultured on TDZ-IBA containing medium also yielded high shoot regeneration frequency that was recorded in the range of 77.77-94.44 % (Koca and Aasim, 2015). Shoot regeneration frequency of 44-100% was reported in T. copticum using cotyledonary node explant (Teymourian et al, 2017). Whereas, 41.1-92.6% shoot regeneration frequency was reported from zygotic embryos explant of T. ammi .…”

“…13.88-80.00 %. Callus induction in response to different PGRs (combination of auxin and cytokinin) has been reported from hypocotyl explant of C. coptimum (Niazian et al, 2017b) cotyledonary node explant of T. copticum (Teymourian et al 2017), hypocotyl and zygotic embryos ) of and T. ammi.…”

“…On the other hand, combination of TDZ-IBA yielded 4.00-8.5 shoots per explant with maximum shoot counts (8.25) were recorded on Ms medium enriched with 0.40 mg/L TDZ+0.10 mg/L IBA. The study by Teymourian et al (2017) revealed shoot count ranged 1.1-3.5 (shoots per explant) from different culture medium with maximum from medium containing no PGR. Shoot length of in vitro regenerated shoots was also showed the clear impact of TDZ-IBA concentration.…”

“…Among these techniques, plant tissue culture is major and these techniques are the most effective for the manipulation of significant seccondary metabolites. In recent years, in vitro propagation of Bishop's weed have been developed for callus induction, somatic embryogenesis, direct or indirect shoot induction (Koca and Aasim 2015;Niazian et al 2017b;Teymourian et al, 2017;. However, there is always need of new in vitro regeneration protocols for medicinal plants.…”