In vitro polyploidisation of Helleborus species

Dhooghe, Emmy; Grunewald, Wim; Leus, Leen; Labeke, Marie-Christine Van

doi:10.1007/s10681-008-9763-9

Cited by 38 publications

(30 citation statements)

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“…For example, artificially induced tetraploid Rhododendrons produced larger flowers (Paden et al 1990), while mitotic chromosome doubling in carnation (Yamaguchi 1989) and cyclamen (Takamura and Miyajima 1996) resulted in deeper-colored flowers. Moreover polyploidisation can overcome one-locus gametophytic self-incompatibility (de Nettancourt 2001;Mable 2004), a characteristic often observed in Ranunculus asiaticus (Horovitz 1985) including the in vitro line 'Alfa' used in this study (personal observations).…”

Section: Introductionmentioning

confidence: 76%

In vitro induction of tetraploids in ornamental Ranunculus

Dhooghe

Denis

Eeckhaut³

et al. 2009

Euphytica

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This study investigates the capacity of the antimitotic agents colchicine, oryzalin and trifluralin for inducing polyploidisation of Ranunculus asiaticus 'Alfa' in vitro shoots. Flow cytometry was used to evaluate the optimal concentration of each antimitotic agent for polyploidisation. Trifluralin at a concentration of 2 mu M resulted in the highest percentage of polyploidisation (27.5%), followed by a colchicine treatment of 200 mu M, which induced 23.3% of polyploids. For oryzalin the highest percentage was achieved using a concentration of 1 mu M. Different exposure periods were tested and turned out to be an important factor. The maximal exposure period tested (10 weeks) resulted in a significant increase in polyploidisation by oryzalin and trifluralin. In contrast, for colchicine (100 mu M) exposure times of either 16 or 24 h did not significantly influence polyploidisation. Additionally the effect of the antimitotic agents on the viability was analysed. For colchicine no significant effect on the survival rate was observed, for trifluralin only a concentration of 10 mu M affected viability whereas for oryzalin, concentration as well as exposure period were significant parameters. Flow cytometric data were confirmed by counting chromosomes in root tip cells

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Section: Introductionmentioning

confidence: 76%

In vitro induction of tetraploids in ornamental Ranunculus

Dhooghe

Denis

Eeckhaut³

et al. 2009

Euphytica

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“…Obtained tetraploid and diploid control plants were rooted in vitro and finally acclimatized in the greenhouse. Ploidy analyses were performed after induction of polyploidy in tissue culture and, as a second control, on the rooted greenhouse plants on a flow cytometer Cyflow Space (Partec, Münster, Germany) as described by Dhooghe et al (2009).…”

Section: Methodsmentioning

confidence: 99%

“…Cells were counted on a flow cytometer. Samples were prepared as described by Dhooghe et al (2009). From 3 plants, leaf discs of 4 mm diameter were taken in 10 biological replicates and the weight per disc was determined.…”

Section: Methodsmentioning

confidence: 99%

Effect of polyploidization on morphology in two apple (Malus × domestica) genotypes

Hias¹,

Leus²,

Davey³

et al. 2017

Hortic. Sci.

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Hias N., Leus L., Davey M.W., Vanderzande S., Van Huylenbroeck J., Keulemans J. (2017): Effect of polyploidization on morphology in two apple (Malus × domestica) genotypes. Hort. Sci. (Prague), 44: 55-63.Because polyploidy often results in enhancement of desirable properties, artificial genome doubling is commonly used in agri-and horticultural crop breeding programs. In this study genome doubling was induced in two apple genotypes. The effect on vegetative morphological and physiological traits of the plants was then comprehensively determined by comparing the obtained tetraploid apple plants with their diploid counterparts. Out of 17 different physio-and morphological characteristics, 15 were significantly affected in one or both genotypes. The response of these 15 characteristics also appeared to have been caused by two effects; 10 of the 15 characteristics exhibited a common response to ploidy change over both genotypes while five traits showed a genotype-specific response to polyploidization. Tetraploid leaves also exhibited a darker leaf colour, which could be correlated to a higher pigment concentration. Furthermore, the results also show a decreased elongation rate and leaf size in tetraploids, which is suggested to be due to the observed lower cell density in the polyploid apple plants.

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“…Although the existence of 2n gametes has been known for a long time, it was assumed that the production of these gametes was rather sporadic and not useful for plant breeding (Ramanna and Jacobsen, 2003). New methods for synthetic polyploidization of plants through the use of spindle inhibitors, such as colchicine or dinitroanilines (for example, oryzalin and trifluralin), have been developed and successfully used in several plants (Vaughn and Lehnen, 1991;Hancock, 1997;Zlesak et al, 2005;Dhooghe et al, 2009). However, synthetic polyploidization has contributed little to crop improvement, likely owing to fixed heterozygosity (Soltis and Soltis, 2000).…”

Section: Introductionmentioning

confidence: 99%