In vitro pre-vascularisation of tissue-engineered constructs A co-culture perspective

Baldwin, Jeremy; Antille, Mélanie; Bonda, Ulrich; De‐Juan‐Pardo, Elena M.; Khosrotehrani, Kiarash; Ivanovski, Sašo; Petcu, Eugen Bogdan; Hutmacher, Dietmar W.

doi:10.1186/2045-824x-6-13

Cited by 86 publications

(78 citation statements)

References 157 publications

(149 reference statements)

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“…25,26 Adding multiple cell types in an organoid presents a challenge regarding culture conditions. 27 In addition, in our system, culture conditions needed to be modified to sustain growth and differentiation of both cell types. We observed that endothelial cells fail to survive and form networks in the optimal culture condition for fibrin BAMs in skeletal muscle growth and differentiation medium.…”

Section: Discussionmentioning

confidence: 99%

Endothelial Network Formation Within Human Tissue-Engineered Skeletal Muscle

Gholobova

Decroix

Muylder

et al. 2015

Tissue Engineering Part A

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Section: Discussionmentioning

confidence: 99%

Endothelial Network Formation Within Human Tissue-Engineered Skeletal Muscle

Gholobova

Decroix

Muylder

et al. 2015

Tissue Engineering Part A

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“…The focus of this section is discussing the materials and microfabrication approaches to generate microvascular systems. Other review papers are recommended for detailed analyses of cell sources and biochemical modification of scaffolds (Bae, Puranik, 2012, Baldwin, Antille, 2014). …”

Section: Structural Mechanical and Microfabrication Considerationmentioning

confidence: 99%

“…Several literature reviews have largely focused on identifying critical aspects for successful development of vascularized models. Many of them were found to be mainly focused on the fundamental principles of engineering, microfabrication techniques (Auger et al, 2013) and microfluidic technologies (Inamdar and Borenstein, 2011, Wong et al, 2012), while other reviews highlighted the pivotal role of cell sources (Baldwin et al, 2014), scaffolds and pro-angiogenic factors (Bae et al, 2012, Kaully et al, 2009, Palumbo et al, 2014, Park and Gerecht, 2014) with low emphasis on microfabrication strategies. This review attempts to critically summarize and connect engineering principles and biological considerations as well as highlight how design of the system architecture and selection of the most suitable microfabrication technique are the first steps for analyzing biological phenomena occurring in a specific environment.…”

Section: Introductionmentioning

confidence: 99%

Cell-microenvironment interactions and architectures in microvascular systems

Bersini

Yazdi

Talò

et al. 2016

Biotechnology Advances

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In the past decade, significant advances have been made in the design and optimization of novel biomaterials and microfabrication techniques to generate vascularized tissues. Novel microfluidic systems have facilitated the development and optimization of in vitro models for exploring the complex pathophysiological phenomena that occur inside a microvascular environment. To date, most of these models have focused on engineering of increasingly complex systems, rather than analyzing the molecular and cellular mechanisms that drive microvascular network morphogenesis and remodeling. In fact, mutual interactions among endothelial cells (ECs), supporting mural cells and organ-specific cells, as well as between ECs and the extracellular matrix, are key driving forces for vascularization. This review focuses on the integration of materials science, microengineering and vascular biology for the development of in vitro microvascular systems. Various approaches currently being applied to study cell-cell/cell-matrix interactions, as well as biochemical/biophysical cues promoting vascularization and their impact on microvascular network formation, will be identified and discussed. Finally, this review will explore in vitro applications of microvascular systems, in vivo integration of transplanted vascularized tissues, and the important challenges for vascularization and controlling the microcirculatory system within the engineered tissues, especially for microfabrication approaches. It is likely that existing models and more complex models will further our understanding of the key elements of vascular network growth, stabilization and remodeling to translate basic research principles into functional, vascularized tissue constructs for regenerative medicine applications, drug screening and disease models.

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“…Some studies have indicated a positive effect of implanting biomaterial constructs co‐cultured with mesenchymal and vascular cells, where the development of vascularized tissues both in vitro and in vivo was enabled 25. Therefore, co‐culturing hMSCs with HUVECs in our study was conducted to generate the vascularized bone tissue.…”

Section: Resultsmentioning

confidence: 97%

Biologically Inspired Smart Release System Based on 3D Bioprinted Perfused Scaffold for Vascularized Tissue Regeneration

et al. 2016

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A critical challenge to the development of large‐scale artificial tissue grafts for defect reconstruction is vascularization of the tissue construct. As an emerging tissue/organ manufacturing technique, 3D bioprinting offers great precision in controlling the internal architecture of a scaffold with preferable mechanical strength and printing complicated microstructures comparable to native tissue. However, current bioprinting techniques still exhibit difficulty in achieving biomimetic nano resolution and cooperating with bioactive spatiotemporal signals. In this study, a comprehensive design of engineered vascularized bone construct is presented for the first time by integrating biomimetic 3D bioprinted fluid perfused microstructure with biologically inspired smart release nanocoating, which is regarded as an aspiring concept combining engineering, biological, and material science. In this biologically inspired design, angiogenesis and osteogenesis are successively induced through a matrix metalloprotease 2 regulative mechanism by delivering dual growth factors with sequential release in spatiotemporal coordination. Availability of this system is evaluated in dynamic culture condition, which is similar to fluid surrounding in vivo, as an alternative animal model study. Results, particularly from co‐cultured dynamically samples demonstrate excellent bioactivity and vascularized bone forming potential of nanocoating modified 3D bioprinted scaffolds for human bone marrow mesenchymal stem cells and human umbilical vein endothelial cells.

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In vitro pre-vascularisation of tissue-engineered constructs A co-culture perspective

Cited by 86 publications

References 157 publications

Endothelial Network Formation Within Human Tissue-Engineered Skeletal Muscle

Endothelial Network Formation Within Human Tissue-Engineered Skeletal Muscle

Cell-microenvironment interactions and architectures in microvascular systems

Biologically Inspired Smart Release System Based on 3D Bioprinted Perfused Scaffold for Vascularized Tissue Regeneration

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