In Vitro Prebiotic and Anti-Colon Cancer Activities of Agar-Derived Sugars from Red Seaweeds

Yun, Eun Ju; Yu, Sora; Kim, Young Ah; Liu, Jing Jing; Kang, Nam Joo; Jin, Yong Su; Kim, Kyoung Heon

doi:10.3390/md19040213

Cited by 23 publications

(14 citation statements)

References 34 publications

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“…It is speculated that this effect may be related to biologically active agarose components enriched from red seaweed. Upregulation of caspase-3, Bax, and caspase-9 expression and downregulation of Bcl-2 and Bcl-xL were observed in HCT-116 cells after AHG treatment (30). Therefore, the growth of human colon cancer HCT-116 cells was effectively suppressed by AHG, indicating that AHG is a potential alternative as an anti-CRC agent.…”

Section: Agarosementioning

confidence: 90%

Anti-colorectal cancer effects of seaweed-derived bioactive compounds

Xie

Zhu

et al. 2022

Front. Med.

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Seaweeds are classified as Chlorophyta, Rhodophyta, and Phaeophyta. They constitute a number of the most significant repositories of new therapeutic compounds for human use. Seaweed has been proven to possess diverse bioactive properties, which include anticancer properties. The present review focuses on colorectal cancer, which is a primary cause of cancer-related mortality in humans. In addition, it discusses various compounds derived from a series of seaweeds that have been shown to eradicate or slow the progression of cancer. Therapeutic compounds extracted from seaweed have shown activity against colorectal cancer. Furthermore, the mechanisms through which these compounds can induce apoptosis in vitro and in vivo were reviewed. This review emphasizes the potential utility of seaweeds as anticancer agents through the consideration of the capability of compounds present in seaweeds to fight against colorectal cancer.

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Section: Agarosementioning

confidence: 90%

Anti-colorectal cancer effects of seaweed-derived bioactive compounds

Xie

Zhu

et al. 2022

Front. Med.

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“…Recently, it was demonstrated that in addition to B. infantis ATCC 15697, other probiotic Bifidobacterium strains assimilating HMOs also have an agarolytic β-galactosidase activity 43 . Second, this unique microbial symbiosis between two distinct gut bacteria, B. plebeius and B. infantis , enables the production of AHG, a rare sugar possessing various physiological activities 23 , 43 , through the degradation of seaweed agarose (Fig. 5 ).…”

Section: Discussionmentioning

confidence: 99%

“…B. infantis ATCC 15697 is isolated from the intestine of infant ( https://www.atcc.org/products/15697 ) where HMOs are abundant, and it is known as a typical member of the gastrointestinal microbiota of breastfed infants 44 . Furthermore, for the cultivation of B. infantis ATCC 15697 , HMOs such as lacto- N -tetraose, lacto- N -neotetraose, and 2′-fucosyllactose, are the preferred carbon sources than AgaDP3 in terms of cell growth rate and maximum cell density measured at OD 600 43 , 45 ; this implies that Bga42A and Bga2A participate in the degradation of HMOs 40 and AgaDP3, but they favorably degrade HMOs than AgaDP3.…”

Section: Discussionmentioning

confidence: 99%

Metabolic and enzymatic elucidation of cooperative degradation of red seaweed agarose by two human gut bacteria

Yun

Park

et al. 2021

Sci Rep

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Various health beneficial outcomes associated with red seaweeds, especially their polysaccharides, have been claimed, but the molecular pathway of how red seaweed polysaccharides are degraded and utilized by cooperative actions of human gut bacteria has not been elucidated. Here, we investigated the enzymatic and metabolic cooperation between two human gut symbionts, Bacteroides plebeius and Bifidobacterium longum ssp. infantis, with regard to the degradation of agarose, the main carbohydrate of red seaweed. More specifically, B. plebeius initially decomposed agarose into agarotriose by the actions of the enzymes belonging to glycoside hydrolase (GH) families 16 and 117 (i.e., BpGH16A and BpGH117) located in the polysaccharide utilization locus, a specific gene cluster for red seaweed carbohydrates. Then, B. infantis extracted energy from agarotriose by the actions of two agarolytic β-galactosidases (i.e., Bga42A and Bga2A) and produced neoagarobiose. B. plebeius ultimately acted on neoagarobiose by BpGH117, resulting in the production of 3,6-anhydro-l-galactose, a monomeric sugar possessing anti-inflammatory activity. Our discovery of the cooperative actions of the two human gut symbionts on agarose degradation and the identification of the related enzyme genes and metabolic intermediates generated during the metabolic processes provide a molecular basis for agarose degradation by gut bacteria.

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“…Bioactive compounds have also been found in polymers from red seaweeds. Some of the properties are antioxidant activity [13], prebiotic activity [14] and others. In this context, biomedical applications such as anti-inflammatory activity [15], antiviral activity [16], antibacterial activity [17], antimicrobial activity [18] and antitumoral activity [14], among others, [1] have been reported; particulate systems with biomedical applications could also be another possibility for delivering compounds that are of in this field [19,20].…”

Section: Introductionmentioning

confidence: 99%

Green Extraction of Carrageenans from Mastocarpus stellatus

et al. 2022

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The recovery of biopolymers from natural resources using eco-friendly extraction technologies that enhance their mechanical properties has gained attention in recent years. In this context, this work deals with the isolation of hybrid carrageenans from Mastocarpus stellatus red seaweed using subcritical water extraction operating in a wide range of thermal conditions (70–190 °C). The extracted biopolymers were analyzed by means of either Fourier-Transform infrared, nuclear magnetic resonance, rheological or cell viability assays. In parallel, the fundamental chemical composition of the seaweed used as raw material, as well as the main phytochemical properties of the soluble liquid extracts, were also studied. Results indicated that thermal extraction conditions significantly affected the rheological behavior of the recovered hybrid carrageenans. The hybrid carrageenan extraction yields varied, with results between 10.2 and 30.2% being the highest values obtained at hydrothermal treatment of 130 °C. A wide palette of viscous features was identified for recovered hybrid carrageenans, with the strongest rheology properties observed at the same temperature. It should be remarked that the maximum inhibitory effect was also obtained at 130 °C for both the ovarian carcinoma cell line (A2780) (65%, IC50: 0.31 mg/mL) and lung carcinoma cell line (A549) (59%, IC50: 0.41 mg/mL).

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In Vitro Prebiotic and Anti-Colon Cancer Activities of Agar-Derived Sugars from Red Seaweeds

Cited by 23 publications

References 34 publications

Anti-colorectal cancer effects of seaweed-derived bioactive compounds

Anti-colorectal cancer effects of seaweed-derived bioactive compounds

Metabolic and enzymatic elucidation of cooperative degradation of red seaweed agarose by two human gut bacteria

Green Extraction of Carrageenans from Mastocarpus stellatus

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