In Vitro Probiotic Properties of Indigenous Dadih Lactic Acid Bacteria

Abstract

The aim of this research was to identify candidate probiotic lactic bacteria among indigenous dadih lactic isolates.Dadih is an Indonesian traditional fermented milk of West Sumatra which is fermented naturally. Viability of the strain is critical in determining the capacity of lactic bacteria to induce immune stimulation as well as to colonize in the intestinal tract. Therefore, LAB are proposed to exert health promoting or probiotic effects in human, such as inhibition of pathogenic microflora, antimutagenic, and the reduction of cholesterol levels. This manuscript reports in vitro probiotic properties of indigenous dadih lactic bacteria, especially some important colonization factors in GI tract, such as lysozyme, acid and bile tolerance. Bile Salt Hydrolase (BSH) activity, spectrum of bacteriocin, and antimutagenic activity of bacterial cells were also assessed. Twenty dadih lactic isolates were screened further for their tolerance to low pH, at pH 2 and 3 as well as their bile tolerance. There were ten isolates classified as acid and bile acid tolerant, and further screened for lysozyme tolerance, BSH activity. The spectrum of bacteriocin activity of isolates was assayed using cell-free neutralized supernatants by agar spot test against variety of pathogens. Lc. lactis subsp.

Butyric Acid as an Antibiotic Substitute for Broiler Chicken A Review

“…Beneficial bacteria like Lactobacillus sp. havehigh bile salt hydrolytic activity which is responsible for deconjugation of bile salts (Sarono, 2003). Deconjugated bile acids are less soluble, hence less absorbed in the intestine and are more likely to excrete cholesterol and its fractionin faeces (Klaver and Van der meer, 1993) thus reduction of cholesterol accretion in the body.…”

Comparative analysis of body weight and serum biochemistry in broilers supplemented with some selected probiotics and antibiotic growth promoters

“…The statistical difference among treated groups were insignificant (P>0.05) ( Table 2). The mechanisms by which probiotics decreases total cholesterol and triglyceride may include their property to deconjugate bile acids enzymatically using bile-salt hydrolase (Surono, 2003). Probiotic microorganisms suppress hydroxy-methyl-glutaryl-coenzyme A, which is an enzyme necessary for cholesterol synthesis pathway thereby, lessen cholesterol synthesis (Fukushima and Nakano, 1995).…”

Lactobacillus plantarum PADA FESES INDIVIDU DEWASA SEHAT YANG MENGONSUMSI Lactobacillus plantarum IS-10506 DARI DADIH

“…cremoris (Surono, 2003). Bagi penderita "lactose intolerance", mengonsumsi dadih merupakan salah satu alternatif untuk memperoleh manfaat dari susu.…”

“…Oleh karena itu, dibutuhkan penelitian tentang keberadaan dan peningkatan jumlah Lactobacillus plantarum IS-10506 pada saluran cerna yang menunjukkan adanya pertumbuhan, sebagai salah satu persyaratan pemanfaatan bakteri ini sebagai probiotik (Surono, 2003; …”