In-vitro processing of sperm with autoantibodies and in-vitro fertilization results

Almeida, Marta; Gazagne, I.; Jeulin, C.; Herry, M.; Belaisch-Allart, J.; Frydman, R.; Jouannet, Pierre; Testart, J

doi:10.1093/oxfordjournals.humrep.a136844

Cited by 98 publications

(38 citation statements)

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“…There appears to be some interaction between the cervical mucus and the Fc region of IgA antibodies [18]. Anti-sperm antibody binding to human sperm inhibits capacitation [19], premature induction of the acrosome reaction, or impairment of zona binding or fertilization of the ova [20][21][22].…”

Section: Discussionmentioning

confidence: 99%

Investigation on the Histopathological Effects of Thyroidectomy on the Seminiferous Tubules of Immature and Adult Rats

Öncü

Kavaklı²,

Gökçimen³

et al. 2004

Urol Int

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Introduction: This study aimed to investigate the histopathological effects of thyroidectomy on both immature and adult rat testes. Materials and Methods: Male albino Wistar rats, 4 weeks old and weighing between 45 and 55 g, were used for this study. The experimental groups were as follows: 2-week control group (group I); 2-week thyroidectomy group (group II); 4-week control group (group III); 4-week thyroidectomy group (group IV); 6-week control group (group V), and 6-week thyroidectomy group (group VI). The control groups included both sham-operated and untreated rats. In groups II, IV and VI, total thyroidectomy was performed under ether anesthesia in all rats at 4 weeks of age. The rats were killed in the 2nd, 4th and 6th weeks, respectively, following the thyroidectomy. The testes of each animal were evaluated histologically. Results: In group II, spermatogenesis progressed to meiosis but round spermatids were found to be decreased and pachytene spermatocytes were observed to be increased when compared to group I. Giant pachytene spermatocytes were seen. There were also many degenerated cells of intermediate origin in the seminiferous epithelium. In groups IV and VI, spermatogonia and primary spermatocytes were normal in appearance, but there was widespread degeneration of the other spermatogenic cells. In addition, some closed lumina covered by degenerated and dead cells were observed. In group II, the mean outer diameter, luminal diameter and area occupied by seminiferous epithelium decreased by 19.74, 32.18, and 28.12%, respectively. In group IV, these data decreased by 23.9, 16.52, and 48.5%, respectively, and in group VI, by 21.10, 19.76 and 40.29%, respectively, when compared with the control groups. These data were statistically significant (p < 0.001). Conclusions: Thyroid hormones could have a marked influence on the seminiferous tubules of both immature and adult rats, and their permanent lack results in a depression in seminiferous tubule growth as shown by the reduced outer and luminal diameters and area occupied by the seminiferous epithelium, which could give rise to degenerative changes in the spermatogenic cells of thyroidectomized rats. In addition, all these changes could also result from both the inability of Sertoli cells to support spermatogenic cells and the diminished levels of GH and FSH.

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Section: Discussionmentioning

confidence: 99%

Investigation on the Histopathological Effects of Thyroidectomy on the Seminiferous Tubules of Immature and Adult Rats

Öncü

Kavaklı²,

Gökçimen³

et al. 2004

Urol Int

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“…Simple dilution with culture media was attempted in the past, but results were poor. 21,22 As normal antigen-antibody reactions usually have affinity constants between 10 7 and 10 9 L/mol, a simple washing step was shown to be insufficient for removing antibodies from sperm surface. 23 The swim-up technique has been used as well, with overall disappointing results.…”

Section: B Discussionmentioning

confidence: 99%

“…28 Other therapeutic approaches to overcome ASA-mediated male infertility include oral administration of corticosteroids, in vitro fertilization and intracytoplasmic sperm injection. Therapeutic protocols based on immunossupressive steroids have been discontinued 6,21 due to reports of major side effects with long-term use. 6,29 Conventional in vitro fertilization (IVF) usually results in lower fertilization and cleavage rates in cases involving ASA-mediated infertility.…”

Section: B Discussionmentioning

confidence: 99%

“…6,29 Conventional in vitro fertilization (IVF) usually results in lower fertilization and cleavage rates in cases involving ASA-mediated infertility. 10,11,21,30 Intracytoplasmic sperm injection, on the other hand, has been shown to successfully overcome any deleterious effects of ASA. ICSI has yielded excellent results irrespective of the ASA levels, and results in fertilization rates, embryo development, and pregnancy rates similar to that which has been observed in other categories of male infertility.…”

Section: B Discussionmentioning

confidence: 99%

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Effectiveness of sperm washing by discontinuous density gradient centrifugation to remove antibodies bound to the sperm membrane

Schneider¹,

Feijó²,

Verza³

et al. 2014

Medical Express

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OBJECTIVE:We evaluated the effectiveness of sperm washing using the discontinuous two-layer density gradient centrifugation method to remove antisperm antibodies attached to the sperm surface. METHOD: We prospectively enrolled sixty-six men with unexplained infertility who were seeking evaluation. Each patient delivered one semen specimen for the study. We determined antisperm antibody levels using the direct immunobeads test. Specimens were classified into two groups according to the pre-washing levels of antibody-bound spermatozoa: group 1 (low antisperm antibodies levels, immunobeads test , 20%; n ¼ 54), and group 2 (high antisperm antibodies levels, immunobeads test $ 20%; n ¼ 12). Sperm washing was carried out using the discontinuous two-layer colloidal density gradient centrifugation method. Pre-and post-wash levels of antisperm antibodies were compared in the groups. RESULTS: The pre-and post-wash percentage of spermatozoa with antisperm antibodies attached to their surface was 11% and 6.5% in group 1 (mean difference ¼ 40%; p , 0.01), and 30% and 19.5% in group 2 (mean difference ¼ 36.8%, p ¼ 0.02), respectively. The effectiveness of density gradient centrifugation in removing antisperm antibodies was not different between the groups, but individual variations from 252.3% to 23.9% were observed. CONCLUSIONS: Sperm washing by density gradient centrifugation is an overall effective method to remove antibodies bound to sperm membranes, regardless of the levels of antisperm antibodies in the neat semen. Due to an inter-individual variation in the effectiveness of the method, we recommend that each patient be tested before applying sperm washing by density gradient centrifugation in intrauterine insemination.

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“…However, in some other reports no significant difference was found (125-127). Although in a early report sperm head directed IgA-more than IgG-antibodies seemed to be associated with a reduced fertilization rate (128), in subsequent reports a significant reduction of the fertilization rate was found when sperm were covered both with IgG-and IgA-ASA (119,129,130, and table 2). However, trying to analyse routine IFV results to determine the actual effect of sperm-bound antibodies on fertilization process is difficult because of some serious reasons.…”

Section: Interference With the Fertilization Processmentioning

confidence: 93%

Naturally-occurring antisperm antibodies in men: interference with fertility and implications for treatment

Francavilla¹

1999

Front Biosci

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In-vitro processing of sperm with autoantibodies and in-vitro fertilization results

Cited by 98 publications

References 0 publications

Investigation on the Histopathological Effects of Thyroidectomy on the Seminiferous Tubules of Immature and Adult Rats

Investigation on the Histopathological Effects of Thyroidectomy on the Seminiferous Tubules of Immature and Adult Rats

Effectiveness of sperm washing by discontinuous density gradient centrifugation to remove antibodies bound to the sperm membrane

Naturally-occurring antisperm antibodies in men: interference with fertility and implications for treatment

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