In vitro production of atractylon and β-eudesmol from Atractylodes chinensis by adventitious root culture

Yun, Cholil; Zhao, Zhuowen; Gu, Lin; Zhang, Zhonghua; Wang, Shengfang; Shi, Yan; Miao, Na; Ri, Ilbong; Wang, Wenjie; Wang, Huimei

doi:10.1007/s00253-022-12194-5

Cited by 10 publications

(2 citation statements)

References 33 publications

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“…The difficulty with plant rooting is related not only to the differences in varieties, but also to the type and concentration of hormones added to the medium and the rooting method selected. IBA and NAA are commonly used hormones in rooting culture [23,24]. IBA is mainly used to promote the growth of the taproot [25], while NAA promotes cell division and expansion and induces the formation of adventitious roots [26].…”

Section: Discussionmentioning

confidence: 99%

Shoot Organogenesis and Regeneration from Leaf Seedlings of Diospyros oleifera Cheng

Liu,

Zhou,

Luo

et al. 2023

Plants

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Persimmons (Diospyros) are economically important trees that are widely cultivated for wood production in China, and Diospyros oleifera Cheng is the main persimmon grafting stock. However, an efficient tissue culture system has not been perfected for D. oleifera due to the limits of proliferation and rooting cultures. Therefore, this study examined the effects of different plant growth regulators and concentrations on the primary culture of young embryos, induction of leaf callus, differentiation of adventitious shoots, and rooting culture of D. oleifera. The optimal formula for young embryo germination was 1/2 Murashige and Skoog (MS) medium containing 0.5 mg/L gibberellic acid (GA3); after 25 days, the sprouting rate of the young embryos was 67.3%. The best medium for leaf callus induction was 1/2MS medium containing 2.0 mg/L of 6-benzylaminopurine (6-BA) and 0.5 mg/L of naphthaleneacetic acid (NAA), and the callus induction rate was 88.9%. Then, the callus was transferred to 1/2MS medium containing 2.0 mg/L of zeatin (ZT), 0.5 mg/L of NAA, and 2.0 mg/L of thidiazuron (TDZ) to induce adventitious shoots; after 25 days, 5.4 buds were produced per explant, and the induction rate of the adventitious shoots was 88.3%. The adventitious shoots were transferred to 1/2MS medium containing 2.0 mg/L of ZT, 2.0 mg/L of 6-(γ,γ-dimethylallylamino)purine (2iP), and 0.1 mg/L of indole acetic acid (IAA) for the proliferation culture, for which the multiplication coefficient approached 7.5. After multiplication, the adventitious shoots were inoculated into 1/2MS medium containing 1.0 mg/L of indole butyric acid (IBA), 0.5 mg/L of NAA, and 1.0 mg/L of kinetin (KT); the rooting rate was 60.2%, and the average number of roots was 6.9.

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Section: Discussionmentioning

confidence: 99%

Shoot Organogenesis and Regeneration from Leaf Seedlings of Diospyros oleifera Cheng

Liu,

Zhou,

Luo

et al. 2023

Plants

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“…Atractylodes chinensis is a perennial herbal plant of the Asteraceae family, mainly distributed in East Asian countries [1]. A. chinensis is one of the commonly used bulk herbs, and its dried rhizomes are used as a medicine, and A. chinensis has a high medicinal value [2,3].…”

Section: Introductionmentioning

confidence: 99%

Efficacy and Potential Mechanism of Essential Oils of Three Labiatae Plants against the Pathogenic Fungi of Root Rot Disease in Atractylodes chinensis

Xie,

Si,

Zhang

et al. 2023

Horticulturae

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Atractylodes chinensis has a high medicinal value and is widely cultivated. However, root rot disease seriously affects the yield and quality of A. chinensis. To develop green and safe pesticides, the inhibitory effect of essential oils (EOs) of three Labiatae plants on the pathogenic fungi that causes root rot disease in Atractylodes chinensis was investigated. The results showed that the Origanum vulgare EO and Thymus mongolicus EO exhibited strong inhibitory effects on Fusarium oxysporum, Fusarium solani, and Fusarium redolens, with 100% inhibition rate. The low MIC values of EOs and their main components against the three pathogenic fungi indicated that all of them showed strong fungicidal effects. The MIC values of O. vulgare EO against F. oxysporum, F. solani, and F. redolens were 2.60 mg/mL, 3.13 mg/mL, and 1.56 mg/mL, respectively. Analyses using scanning electron microscopy (SEM) and transmission electron microscopy (TEM) showed that the O. vulgare EO severely damaged the cell wall and cell membrane of mycelial cells. The O. vulgare EO increased cell permeability, leading to a large leakage of cell contents (DNA and proteins). In addition, O. vulgare EO inhibited F. oxysporum by inducing ROS production and reducing the amount of intracellular GSH, leading to a large accumulation of ROS. This study showed that plant EOs have excellent fungicidal activity and can be used as novel natural and environmentally friendly pesticides for the control of root rot in A. chinensis.

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Advanced Systems and Bioreactors for Large-Scale Secondary Metabolite Production in Medicinal Plants Using Suspension Cultured Cells

Xu,

2024

Biosynthesis of Natural Products in Plants

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In vitro production of atractylon and β-eudesmol from Atractylodes chinensis by adventitious root culture

Cited by 10 publications

References 33 publications

Shoot Organogenesis and Regeneration from Leaf Seedlings of Diospyros oleifera Cheng

Shoot Organogenesis and Regeneration from Leaf Seedlings of Diospyros oleifera Cheng

Efficacy and Potential Mechanism of Essential Oils of Three Labiatae Plants against the Pathogenic Fungi of Root Rot Disease in Atractylodes chinensis

Advanced Systems and Bioreactors for Large-Scale Secondary Metabolite Production in Medicinal Plants Using Suspension Cultured Cells

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