In vitro production of bovine embryos: cumulus/granulosa cell gene expression patterns point to early atresia as beneficial for oocyte competence

Mazzoni, Gianluca; Razza, E. M.; Pedersen, Helene Helboe; Secher, Jan Bojsen-Møller; Kadarmideen, Haja N.; Callesen, Henrik; Stroebech, Lotte; Freude, Kristine; Hyttel, Poul

doi:10.21451/1984-3143-ar990

Cited by 3 publications

(1 citation statement)

References 45 publications

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“…It codes for a GTPase protein, which aids in the antiviral response by targeting viral nucleocapsids [ 29 ]. The GTPase also positively correlates with follicular atresia and acts as a marker of oocyte quality, as demonstrated by a 2017 bovine-based study [ 32 ]. The next genes encode proteins of the keratin family.…”

Section: Discussionmentioning

confidence: 99%

Expression Profile of New Gene Markers and Signaling Pathways Involved in Immunological Processes in Human Cumulus-Oophorus Cells

Chermuła

Hutchings

Kranc

et al. 2021

Genes

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The function of the immune system extends from defense against external pathogens to the recognition and elimination of mutated or dying cells, aiding elimination of malignant potential and/or maintaining homeostasis. The many cell types of the immune system secrete a broad range of factors to enable cellular signaling that is vital to physiological processes. Additionally, in the ovary, follicular selection and maturation, as well as ovulation, are directly regulated by the nearby immune cells. Additionally, ovulation and rupture of the follicle have been observed to resemble a local inflammatory response. Cells of the cumulus–oocyte complex (COC) show evolving gene expression profiles throughout the oocytes’ lifespan, including genes associated with immunological processes. Analysis of these genes allows the identification of useful molecular markers, as well as highlighting gene functions and interactions in these cells. Cumulus cells were obtained from hormonally stimulated patients undergoing an in vitro fertilization procedure and studied under long-term culture conditions. The microarray technique made it possible to compare the level of CCs’ gene expression on the 1st, 7th, 15th and 30th day of cultivation. Additionally, RNA microarray analysis was performed to map gene expression in these cells, associated with immunological processes and associated cytokine signaling. Subsequently, the use of DAVID software allowed us to identify the “defense response to other organism”, “defense response”, “defense response to virus”, “cytokine secretion”, “cytokine production” and “cytokine-mediated signaling pathway” GO BP terms, as well as allowing further analysis of the most differentially expressed genes associated with these processes. Of the 122 genes involved, 121 were upregulated and only one was downregulated. The seven most upregulated genes related to the abovementioned terms were ANXA3, IFIT1, HLA-DPA1, MX1, KRT8, HLA-DRA and KRT18. Therefore, genes involved in immunological defense processes are upregulated in CC cultures and could serve as useful molecular markers of growth and development in the COC, as well as the proliferation of granulosa and cumulus cells.

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Section: Discussionmentioning

confidence: 99%

Expression Profile of New Gene Markers and Signaling Pathways Involved in Immunological Processes in Human Cumulus-Oophorus Cells

Chermuła

Hutchings

Kranc

et al. 2021

Genes

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Transcriptomics–genomics data integration and expression quantitative trait loci analyses in oocyte donors and embryo recipients for improving invitro production of dairy cattle embryos

Kadarmideen

Mazzoni

2019

Reprod. Fertil. Dev.

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In this paper we first provide a brief review of main results from our previously published studies on genome-wide gene expression (transcriptomics) in donor and recipient cattle used in invitro production (IVP) of embryos and embryo transfer (ET). Then, we present novel results from applying integrative systems genomics and biological analyses where transcriptomics data are combined with genomic data in both donor and recipient cattle to map expression quantitative trait loci (eQTLs). The eQTLs are genetic markers that can regulate or control the expression of genes in the entire genome, via complex molecular mechanisms, and thus can act as a powerful tool for genomic and gene-assisted selection. We identified significant eQTLs potentially controlling the expression of 13 candidate genes for donor cow quality (IVP parameters; e.g. cyclin B1 (CCNB1), outer dense fiber of sperm tails 2 like (ODF2L)) and 19 candidate genes for recipient cows quality (endometrial receptivity; e.g. ER membrane protein complex subunit 9 (EMC9), mannosidase beta (MANBA), peptidase inhibitor 16 (PI16)). Annotation and colocation of detected eQTLs show that some of the eQTLs are in the same genomic regions previously reported as QTLs for reproduction-related traits. However, eQTLs and the candidate genes identified should be further validated in larger populations before implementation as genetic markers or used in genomic selection for improving IVP and ET performance.

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Assessment of the destructive processes of chromatin of granulosa cells and functional status of oocyte in bovine ovarian follicles

Stanislavovich¹,

Kuzmina²,

Molchanov³

2019

Agrarian Bulletin of The

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Abstract. Currently, there is the possibility of more detailed studies to the study of oocytes and somatic cells (granulosa cells). The possibility to develop successful models of maturation of female gametes defines the possibility to improve existing methods for the selection of donor eggs and the search for new donor eggs. Oocyte maturation in vivo occurs with the participation of structural follicle elements and follicular fluid [3, 4, 6]. Granulosa cells are widely used in bovine oocyte maturation systems and used in cloning and transgenesis technologies [8, 13]. Purpose of this study: to perform destructive changes granulosa cells in ovarian follicles of bovines (Ø 3–5 mm),which contain growing(ВСВ–) or completed the growth phase of oocytes (ВСВ+). Methods: Functional testing of oocytes wascarried out using the vital dye BCB (brillant cresyl blue – diamond crystal blue) [10]. Viability indices in granulosa cells isolated from follicles that contain oocyte s that grow or complete the growth phase were determined by flow cytometry. The result. It was found, that cellsof granulosa from cow follicles are characterized by different indicators of apoptosis levels depending on the status of oocytes (completed growth and growing) isolated from these follicles. The proportion of apoptotic granulosa cells in bovine follicles of containing oocytes that completed the growth phase, exceeded that in follicles containing growing oocytes by 11 % (29 % vs. 18 %, c:dP < 0.05). The scientific novelty: The data obtained using flow cytometry, allow us to evaluate the level of apoptosis in granulosa cells of bovine ovarian follicle as indicator of functional status of developing oocytes (growing or completed growth phases). This indicator can be used in prognosis of competencies for maturation of bovine oocytes.

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In vitro production of bovine embryos: cumulus/granulosa cell gene expression patterns point to early atresia as beneficial for oocyte competence

Cited by 3 publications

References 45 publications

Expression Profile of New Gene Markers and Signaling Pathways Involved in Immunological Processes in Human Cumulus-Oophorus Cells

Expression Profile of New Gene Markers and Signaling Pathways Involved in Immunological Processes in Human Cumulus-Oophorus Cells

Transcriptomics–genomics data integration and expression quantitative trait loci analyses in oocyte donors and embryo recipients for improving invitro production of dairy cattle embryos

Assessment of the destructive processes of chromatin of granulosa cells and functional status of oocyte in bovine ovarian follicles

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