In vitro production of cloned plants of jojoba (Simmondsia chinensis (link) Schneider) through shoot proliferation in long-term culture

Chaturvedi, H. C.; Sharma, Meena

doi:10.1016/0168-9452(89)90245-8

Cited by 44 publications

(30 citation statements)

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“…Moreover, jojoba plants derived from tissue culture grow more vigorously than seedlings or rooted cuttings, and yield a significantly larger population after first year growth. Some reports on in vitro propagation using various explants such as auxiliary buds (Chaturvedi and Sharma 1989, Llorente and Apostolo 1998), nodal explants (Tyagi and Prakash 2004, Hegazi et al 2014) and shoot tips (Sardana and Batra 1998) are available. In vitro rooting percentage ranged from 20 and 95% depending on the clone and the specific trial.…”

Section: Introductionmentioning

confidence: 99%

Micropropagation and Assessment of Genetic Stability of In Vitro Raised Jojoba (Simmondsia chinensis Link.) Plants Using SCoT and ISSR Markers

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Gabr

Reda

et al. 2016

Plant Tissue Cult. & Biotech.

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This study is aimed at developing an efficient method for micropropagation of true-to-type jojoba plants. Nodal segments were used for in vitro shoots proliferation. Among three concentrations of BA used for multiplication, 1 mg/l BA gave the highest number of shoots. To enhance growth of shoots, combinations of BA and Kn were investigated. The greater value of shoot length and the maximum number of nodes were observed in the medium containing 1 mg/l BA + 1.5 mg/l Kn. Among different medium used to increase the rate of multiplication, the maximum number of shoots was recorded at ½ MS + Gamborg B5 (B5) vitamins + 2 mg/l Kn + 10 mg/l adenine sulphate (AS). Rooting was obtained upon supplementation of ½ Woody Plant Medium (WPM) with 1 mg/l IBA. Acclimation was achieved by transplanting rooted plantlets into pots containing peat moss and vermiculite. Start codon targeted (SCoT) and intersimple sequence repeat (ISSR) analyses were carried out to assess the genetic fidelity of micropropagated plantlets. All banding profiles of the two analyses from micropropagated plants were monomorphic and similar to the mother plant. Hence, this protocol can be employed for commercial micropropagation of jojoba without the risk of losing genetic stability.

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Section: Introductionmentioning

confidence: 99%

Micropropagation and Assessment of Genetic Stability of In Vitro Raised Jojoba (Simmondsia chinensis Link.) Plants Using SCoT and ISSR Markers

Bekheet

Gabr

Reda

et al. 2016

Plant Tissue Cult. & Biotech.

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“…Similar results were reported in Camellia sinensis Linnaeus and Musa acuminata Colla (Mondal et al 1998;Farahani et al 2008). The effect of alone 6-benzylaminopurine (BAP) and in combination with auxin on micropropagation of Jojoba has been documented by different rates of shoot multiplication (Driver, Kuniyuki 1984;Chaturvedi, Sharma 1989;Mills et al 1997;Llorente, Apóstolo 1998;Tyagi, Prakash 2004;Singh et al 2008). In the present study, TDZ alone could give rise to 20 shoots.…”

Section: Discussionmentioning

confidence: 62%

“…BAP is reported to produce a higher number of shoots per explant, however, it induces callus and hyperhydrated shoots (Llorente, Apóstolo 1998, 2000Mills et al 2004;Tyagi, Prakash 2004). We could achieve more than 90% in vitro rooting, which was higher than earlier (Chaturvedi, Sharma 1989;Mills et al 1997;Tyagi, Prakash 2004). The rooting percentage of shoots increased from around 39 to 92% from the first to the third subculture, hence from the 3 rd culture onwards shoots are more suitable for rooting, which may be due to the better physiological condition of shoots for better rooting.…”

Section: Discussionmentioning

confidence: 81%

“…Jojoba can be propagated through seeds but due to low and inconsistent seed yield the cultivations were not taken up as expected (Chaturvedi, Sharma 1989;Coates et al 2006). Vegetative propagation could be used for the generation of selected clones with limited success (Loreto et al 1998).…”

mentioning

confidence: 99%

“…Vegetative propagation could be used for the generation of selected clones with limited success (Loreto et al 1998). In order to overcome the difficulties in vegetative propagation methods for micropropagation have been developed using an in vitro rooting method (Chaturvedi, Sharma 1989;Mills et al 1997;Llorente, Apóstolo 1998;Roussos et al 1999;Hamama et al 2001;Tyagi, Prakash 2004;Llorente et al 2007;Singh et al 2008). Ex vitro rooting is a promising method, skipping the phase of in vitro rooting, shortens the micropropagation cycle by 2-3 weeks or more and so minimizes the time period of the plantlet generation (Debergh, Maene 1981;Martin 2003).…”

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confidence: 99%

See 2 more Smart Citations

Enhanced micropropagation protocol of ex vitro rooting of a commercially important crop plant Simmondsia chinensis (Link) Schneider

Singh¹,

Agarwal²

2016

J. For. Sci.

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A micropropagation protocol was developed by further improvement of prevailing methods using proven germplasm for ex vitro rooting and addressed the effect of the number of subcultures on the rooting ability of shoots. A comparative study was done between in vitro rooting method and ex vitro rooting method. Using the ex vitro rooting method a plantlet could be produced in 135 days, which was in a shorter time compared to the in vitro rooting method -180 days. The best axillary shoot bud induction was observed on Murashige and Skoog (MS) medium supplemented with 4.6 µM thidiazuron (TDZ) with 5 shoot buds per explant. In the shoot cluster, which was subcultured on MS medium supplemented with 2.3 µM TDZ, the rate of shoot multiplication increased in the 3 rd subculture. The maximum mean number of shoots per explant (20) was obtained at the 3 rd subculture on the same medium. Shoots were harvested at the 1 st , 3 rd and 5 th subculture and pulse treated for root induction. The highest rooting (95%) was achieved from the 3 rd subculture onwards with pulse treated shoots for fifteen days. The rooted plants could be established in a greenhouse with 99% survival. Ex vitro rooting is a promising method to reduce the time for plant generation. The resultant plantlets well established in pots and fruiting was observed within a year.

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In Vitro Propagation of Jojoba

Llorente

Apóstolo

2012

Methods in Molecular Biology

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Jojoba (Simmondsia chinensis (Link) Schn.) is a nontraditional crop in arid and semi-arid areas. Vegetative propagation can be achieved by layering, grafting, or rooting semi-hardwood cuttings, but the highest number of possible propagules is limited by the size of the plants and time of the year. Micropropagation is highly recommended strategy for obtaining jojoba elite clones. For culture initiation, single-node explants are cultivated on Murashige and Skoog medium (MS) supplemented with Gamborg's vitamins (B5), 11.1 μM BA (N(6)-benzyl-adenine), 0.5 μM IBA (indole-3-butyric acid), and 1.4 μM GA(3) (gibberellic acid). Internodal and apical cuttings proliferate on MS medium containing B5 vitamins and 4.4 μM BA. Rooting is achieved on MS medium (half strength mineral salt) amended with B5 vitamins and 14.7 μM IBA during 7 days and transferred to develop in auxin-free rooting medium. Plantlets are acclimatized using a graduated humidity regime on soil: peat: perlite (5:1:1) substrate. This micropagation protocol produces large numbers of uniform plants from selected genotypes of jojoba.

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In vitro production of cloned plants of jojoba (Simmondsia chinensis (link) Schneider) through shoot proliferation in long-term culture

Cited by 44 publications

References 5 publications

Micropropagation and Assessment of Genetic Stability of In Vitro Raised Jojoba (Simmondsia chinensis Link.) Plants Using SCoT and ISSR Markers

Micropropagation and Assessment of Genetic Stability of In Vitro Raised Jojoba (Simmondsia chinensis Link.) Plants Using SCoT and ISSR Markers

Enhanced micropropagation protocol of ex vitro rooting of a commercially important crop plant Simmondsia chinensis (Link) Schneider

In Vitro Propagation of Jojoba

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