In vitro production of high titre monoclonal antibody by hybridoma cells in dialysis culture

Adamson, Sheena; Fitzpatrick, Sandra L.; Behie, Leo A.; Gaucher, G. M.; Lesser, Barry H.

doi:10.1007/bf00130835

Cited by 43 publications

(8 citation statements)

References 6 publications

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“…The maximum cell density of 1-3 x 10 7 cells/ml is the expected level in high. density suspension culture compared with our previous reports Tokashiki, 1987;1988) and other methods such as rotating filter (Tolbert, 1981) or dialysis culture (Adamson, 1983).…”

Section: Discussionmentioning

confidence: 95%

High cell density perfusion culture of hybridoma cells recycling high molecular weight components

et al. 1988

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We have developed a high cell density and high product concentration culture system recycling high molecular weight components. The production of monoclonal antibodies in high concentration was performed by this culture system with mouse human hybridoma H2 and V6 cells in serum-free defined media.The concentration of IgG after 48 days culture of H2 cells in ITES-eRDF reached 2 mg/ml and the purity of IgG in culture fluid was 61%. In addition, high molecular weight components in serum-free media, such as transferrin or BSA, could be reduced to 5% of the original concentration.

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Section: Discussionmentioning

confidence: 95%

High cell density perfusion culture of hybridoma cells recycling high molecular weight components

et al. 1988

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“…The concept of continuous filtration to concentrate high-molecular-weight components, that is retaining the product of interest in the bioreactor and eliminating low-molecular-weight components (e.g., toxic by-products, lactate, and ammonium) was introduced in continuous dialysis bioreactor. [17][18][19] The same principle is now used in industry using ultrafiltration (UF) HF perfusion, that is XD Process Technology at DSM, achieving very high cell densities 20 ; however, experimental details have not been disclosed. The advantage of this process is to retain the product of interest inside the bioreactor so that harvesting of the bioreactor is performed once instead of weekly/bi-weekly harvests of the perfused permeate.…”

Section: Introductionmentioning

confidence: 99%

Very high density of Chinese hamster ovary cells in perfusion by alternating tangential flow or tangential flow filtration in WAVE bioreactor™—part II: Applications for antibody production and cryopreservation

Clincke

Mölleryd

Samani

et al. 2013

Biotechnology Progress

134

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A high cell density perfusion process of monoclonal antibody (MAb) producing Chinese hamster ovary (CHO) cells was developed in disposable WAVE Bioreactor™ using external hollow fiber (HF) filter as cell separation device. Tangential flow filtration (TFF) and alternating tangential flow (ATF) systems were compared and process applications of high cell density perfusion were studied here: MAb production and cryopreservation. Operations by perfusion using microfiltration (MF) or ultrafiltration (UF) with ATF or TFF and by fed-batch were compared. Cell densities higher than 108 cells/mL were obtained using UF TFF or UF ATF. The cells produced comparable amounts of MAb in perfusion by ATF or TFF, MF or UF. MAbs were partially retained by the MF using ATF or TFF but more severely using TFF. Consequently, MAbs were lost when cell broth was discarded from the bioreactor in the daily bleeds. The MAb cell-specific productivity was comparable at cell densities up to 1.3 × 108 cells/mL in perfusion and was comparable or lower in fed-batch. After 12 days, six times more MAbs were harvested using perfusion by ATF or TFF with MF or UF, compared to fed-batch and 28× more in a 1-month perfusion at 108 cells/mL density. Pumping at a recirculation rate up to 2.75 L/min did not damage the cells with the present TFF settings with HF short circuited. Cell cryopreservation at 0.5 × 108 and 108 cells/mL was performed using cells from a perfusion run at 108 cells/mL density. Cell resuscitation was very successful, showing that this system was a reliable process for cell bank manufacturing. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 29:768–777, 2013

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“…Several groups have evaluated the dialysis perfusion system as either an internal or external culture system. The internal method cultures hybridoma cells inside the dialysis tubing, which is suspended in medium [38,39]. Unfortunately, the cell compartment is not well mixed and consequently the maximum usable volume is limited.…”

Section: Filtration Systemsmentioning

confidence: 99%

Bioreactor systems for the production of biopharmaceuticals from animal cells

Warnock

Al‐Rubeai

2006

Biotech and App Biochem

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The demand for biopharmaceutical products is set to see a significant increase over the next few years. As a consequence, the processes used to produce these products must be able to meet market requirements. The present paper reviews the current technologies available for animal cell culture and highlights the advantages and disadvantages of each method, while also providing details of recent case studies. Processes are described for both suspension and anchorage-dependent cell lines.

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In vitro production of high titre monoclonal antibody by hybridoma cells in dialysis culture

Cited by 43 publications

References 6 publications

High cell density perfusion culture of hybridoma cells recycling high molecular weight components

High cell density perfusion culture of hybridoma cells recycling high molecular weight components

Very high density of Chinese hamster ovary cells in perfusion by alternating tangential flow or tangential flow filtration in WAVE bioreactor™—part II: Applications for antibody production and cryopreservation

Bioreactor systems for the production of biopharmaceuticals from animal cells

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