In Vitro Production of Late Blight (Phytophthora infestans)-Resistant Potato Plants

Cassells, A. C.; Sen, Paromita

doi:10.1007/978-3-642-61081-3_7

Cited by 1 publication

(1 citation statement)

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“…Particularly if in vitro selection can be used (Jones, 1990). Unfortunately, a selection factor has not yet been characterized for late blight (Wenzel & Foroughi-Wehr, 1993;Cassells & Sen, 1996). It is recognized that mutation breeding gives large numbers of degenerate lines.…”

Section: Introductionmentioning

confidence: 99%

Mutation breeding for yield andPhytophthora infestans (Mont.) de Bary foliar resistance in potato (Solanum tuberosum L. cv. Golden Wonder) using computerized image analysis in selection

Kowalski

Cassells

1999

Potato Res

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SummaryUsing visual selection for off-types and image analysis to select against maturation mutants, a line combining improved late blight resistance and yield was isolated in an in vitro mutagenesis programme from a population of 2101 putative mutants. The adventitious regener.ants from mutagenesis treatment, including spontaneous mutants (somaclonal variants), were subjected to serial subculture of the apical bud in vitro and two cycles of apical cuttings in vivo to breakdown chimeras. The selected line was stable in trials over five years; others were unstable or failed to combine improvement in late blight resistance with high yield. Leaf and tuber image analysis was used to characterize selected lines. Lines that differed most from cv. Golden Wonder in yield and late blight resistance were more easily distinguished from the control by image analysis. The role of image analysis in negative selection and in determination of the relatedness of mutants to the control is discussed.

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Section: Introductionmentioning

confidence: 99%