In vitro production of sex preselected cattle embryos using a monoclonal antibody raised against bull sperm epitopes

Chowdhury, M.M.R.; Xu, Li; Kong, Rami; Park, Bun-Young; Mesalam, Ayman; Joo, Myeong-Don; Afrin, Fahmida; Jin, Jong-In; Lim, Hyun‐Tae; Kong, Il‐Keun

doi:10.1016/j.anireprosci.2018.11.006

Cited by 17 publications

(14 citation statements)

References 17 publications

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“…However, in our results, the cleavage rate was higher in the X-sorted group compared to the Y-sorted group (Table S1). The low cleavage rate in the Y-sorted sperm group was due to the WholeMom antibody, which causes agglutination of the Y spermatozoa and decreases the fertilization capacity by inhibiting the movement of Y sperm [7]. We found that BL expansion and hatching rate was consistently higher in the case of Y sperm-sorted embryos compared to X sperm-produced embryos.…”

Section: Discussionmentioning

confidence: 67%

“…This specificity was confirmed by Y-specific gene expression. Importantly, WholeMom-treated X sperm-sorted group through artificial insemination produced 76% female and 24% male in the field study [7]. This antibody exclusively binds to the head of Y-bearing spermatozoa and cause head-to-head agglutination of the Y sperms.…”

Section: Discussionmentioning

confidence: 99%

“…To analyze the difference in development kinetics among male and female sexed embryos, we compared the formation of an expanded BL ratio between two groups. Sex preselected embryo production via a Y-specific monoclonal antibody yielded more than 70% accuracy in male and female BL development [7]. Among the X-and Y-sorted embryo groups, more than 55% of the Y-sorted embryos rapidly developed to the expanded BL stage at day seven post-fertilization, whereas the X-sorted group produced only 25% expanded BLs at day seven (Table 1).…”

Section: Effect Of Gender Sex On Blastocyst Development Kinetics Commentioning

confidence: 99%

“…For in vitro fertilization, the spermatozoa were sex-sorted with WholeMom antibody, as previously described [7]. The accuracy of formation of male and female embryos was checked by amelogenin (αMEL) primer with RT-PCR, as described by [7]. To make the control set, in vitro fertilization was performed with unsorted semen.…”

Section: In Vitro Fertilization and In Vitro Culturing Of Sex-sorted mentioning

confidence: 99%

“…The rapid growth of pre-implantation embryos is a manifestation of healthy embryos [6]. These rapidly developing embryos are preferentially selected for transfer, cryopreservation, and commercial breeding practices [5,7]. The faster growth rate of male relative to female embryos has led to concern about the in vitro culturing conditions and IVF treatment, which could result in a male-biased sex ratio [8].…”

Section: Introductionmentioning

confidence: 99%

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Difference in Developmental Kinetics of Y-Specific Monoclonal Antibody Sorted Male and Female In Vitro Produced Bovine Embryos

Sidrat

Kong

Khan

et al. 2019

IJMS

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Sex-related growth differences between male and female embryos remain an attractive subject for reproductive biologists. This study aimed to investigate the endogenous factors that play a crucial role in the pace of early development between male and female bovine embryos. Using sex pre-selected semen by Y-specific monoclonal antibodies for the production of bovine embryos, we characterized the critical endogenous factors that are responsible for creating the development differences, especially during the pre-implantation period between male and female embryos. Our results showed that at day seven, (57.8%) Y-sperm sorted in vitro cultured embryos reached the expanded blastocyst (BL) stage, whereas the X-sperm sorted group were only 25%. Y-BLs showed higher mRNA abundance of pluripotency and developmental competency regulators, such as Oct4 and IGF1-R. Interestingly, Y-sperm sorted BLs had a homogeneous mitochondrial distribution pattern, higher mitochondrial membrane potential (∆Ψ m ), efficient OXPHOS (oxidative phosphorylation) system and well-encountered production of ROS (reactive oxygen species) level. Moreover, Y-blastocysts (BLs) showed less utilization of glucose metabolism relative to the X-BLs group. Importantly, both sexes showed differences in the timing of epigenetic events. All these factors directly or indirectly orchestrate the whole embryonic progression and may help in the faster and better quality yield of BL in the Y-sperm sorted group compared to the X counterpart group.

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Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 99%

Section: Effect Of Gender Sex On Blastocyst Development Kinetics Commentioning

confidence: 99%

Section: In Vitro Fertilization and In Vitro Culturing Of Sex-sorted mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Difference in Developmental Kinetics of Y-Specific Monoclonal Antibody Sorted Male and Female In Vitro Produced Bovine Embryos

Sidrat

Kong

Khan

et al. 2019

IJMS

Self Cite

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Bovine semen sexing: Sperm membrane proteomics as candidates for immunological selection of X‐ and Y‐chromosome‐bearing sperm

Quelhas

Santiago

Matos

et al. 2021

Veterinary Medicine & Sci

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The use of sexed semen in dairy and beef farms ensures the production of animals of the desired sex, resulting in a reduction of costs and an improvement of environmental sustainability. Several methods have been developed over the years, but most of them were abandoned due to their limited efficacy. Currently, the only commercially available method for the separation of X‐ and Y‐chromosome‐bearing sperm is fluorescence‐activated cell sorting. However, this technique is expensive and has limited usefulness for the industry, considering that it cannot produce doses of sexed semen with the desired number of sperm for artificial insemination. Immunological methods have emerged as an attractive alternative to flow cytometry and proteomic knowledge of X‐ and Y‐sperm could be useful to the development of a new method. In this review, we identify the main applications of sexed semen, describe the existing methods and highlight future research opportunities in the field. We consider that immunological methods, based on sperm cell's surface proteins differentially expressed between X‐ and Y‐sperm, could be an interesting and promising approach to semen sexing.

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Pre-implantation development of cattle embryos produced from fresh bull semen enriched for X- chromosome-bearing spermatozoa using a monoclonal antibody

Uhm

Heo²,

Dong³

et al. 2023

Vet Res Commun

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In vitro production of sex preselected cattle embryos using a monoclonal antibody raised against bull sperm epitopes

Cited by 17 publications

References 17 publications

Difference in Developmental Kinetics of Y-Specific Monoclonal Antibody Sorted Male and Female In Vitro Produced Bovine Embryos

Difference in Developmental Kinetics of Y-Specific Monoclonal Antibody Sorted Male and Female In Vitro Produced Bovine Embryos

Bovine semen sexing: Sperm membrane proteomics as candidates for immunological selection of X‐ and Y‐chromosome‐bearing sperm

Pre-implantation development of cattle embryos produced from fresh bull semen enriched for X- chromosome-bearing spermatozoa using a monoclonal antibody

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