In Vitro Production of Steroid Cataract in Bovine Lens

Miller, David; Tijerina, Monica; Mayman, Chaim I.

doi:10.1111/j.1755-3768.1979.tb00544.x

Cited by 13 publications

(7 citation statements)

References 6 publications

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“…Dex (4) Oua (4) intervals over this period. These data were correlated with daily light transmission measurements as described in the previous communication (Miller et al 1979). Tables I and I1 show the effect on light transmission and ATPase activity in lenses incubated for three days in dexamethasone or ouabain at concentrations ranging from I X 10-:4 to 1 X 10-7 M. T h e figures are expressed as percentages of the data for the control lenses.…”

Section: Resultsmentioning

confidence: 87%

“…In another series of experiments, fresh calf lenses were prepared and incubated indivi-I n Vitro Sturotri Cntrirrirt I 1 dually in medium containing varying concentrations of dexamethasone in a manner described in the foregoing communication (Miller et al 1979). Lenses were also incubated in media containing similar concentrations of ouahain.…”

Section: Methodsmentioning

confidence: 99%

“…Control incubations of lenses were carried out in identical media containing neither dexamethasone nor ouabain. At intervals during a three-day incubation period, the lenses were removed from the medium and light transmission measured in the manner previously described (Miller et al 1979). After measurement of light transmission, whole lenses were homogenized in 0.05 M tris buffer, p H 7.4 containing 0.05 M EDTA, using a teflon coated homogenizer.…”

Section: Methodsmentioning

confidence: 99%

“…This inhibition is of the same order of magnitude as that of ouabain. Postulating that inhibition of this enzyme in the lens and thereby of the pump should produce cataract formation, Miller et al (1979) reported, in the previous paper, cataract formation when calf lenses were incubated for three days in medium containing 1 x 10-4 M dexamethasone 21-phosphate. T h e cataract thus formed was of the same degree and type as that produced by incubation in equimolar concentrations of ouabain.…”

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confidence: 89%

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In Vitro Production of Steroid Cataract in Bovine Lens

Mayman

Miller

Tijerina

1979

Acta Ophthalmologica

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Fresh calf lesnses incubated in nutritive media containing dexamethasone phosphate or ouabain in concentrations ranging from 1 X 10(-4) M to 1 X 10(-8) M developed cortical opacification and showed significant inhibition of Na(+)-K(+) ATP'ase activity. Over a 3-day incubation period the decrease in Na+-K+ ATP'ase activity correlated well with the observed decrease in light transmission. The degree of enzyme inhibition and decrease in light transmission varied directly with the concentration of dexamethasone phosphate and ouabain, with significant changes observed at 'physiologic' and 'pharmacologic' concentrations of these agents. Lenses incubated for 4 days in dexamethasone phosphate or ouabain showed substantial increases in water content as well as an increase in Na+ and a decrease in K+ concentration. These data suggest that inhibition of the cation pump may play a significant role in the formation of steroid cataract in vitro.

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Section: Resultsmentioning

confidence: 87%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 89%

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In Vitro Production of Steroid Cataract in Bovine Lens

Mayman

Miller

Tijerina

1979

Acta Ophthalmologica

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“…[ 17 ] Decreased lenticular ionic transport resulting from a specific decrease in N a+ K + Adenosine Triphosphatase (ATPase) activity in the lens epithelium leads to cataract formation in the Nakano mouse. [ 18 ] Some in vitro studies with N a+ K + ATPase inhibitors also results in lens opacification. [ 18 ] Low N a+ K + ATPase activity has been reported in renal microsomal preparations from hypertensive Dahl salt-sensitive rats.…”

Section: Risk Factors Of Cataractmentioning

confidence: 99%

Etiopathogenesis of cataract: An appraisal

Gupta

Rajagopala

Ravishankar

2014

Indian J Ophthalmol

152

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Natural eye lens is a crystalline substance to produce a clear passage for light. Cataract is opacity within the clear lens of the eye and is the dominant cause of socio-medical problem i.e., blindness worldwide. The only available treatment of cataract is surgery. However, insufficient surgical facilities in poor and developing countries and post-operative complications inspire researchers to find out other modes of treatment for cataract. In this review, an attempt has been made to appraise various etiological factors of cataract to make their perception clear to build up counterpart treatment. Present study is an assortment of various available literatures and electronic information in view of cataract etiopathogenesis. Various risk factors have been identified in development of cataracts. They can be classified in to genetic factors, ageing (systemic diseases, nutritional and trace metals deficiencies, smoking, oxidative stress etc.), traumatic, complicated (inflammatory and degenerative diseases of eye), metabolic (diabetes, galactosemia etc.), toxic substances including drugs abuses, alcohol etc., radiation (ultraviolet, electromagnetic waves etc.) are implicated as significant risk factors in the development of cataract.

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Expression and Regulation of Sparc, Fibronectin, and Collagen Iv by Dexamethasone in Lens Epithelial Cells

Sawhney

2002

Cell Biology International

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Cataract formation is a deleterious side effect of some hormone therapies, thus, it is important to understand how hormones regulate lens basement structure and function. We have examined the effects of dexamethasone (DEX) on the regulation of Secreted Protein Acidic and Rich in Cysteines (SPARC), fibronectin (FN), and collagen IV (CN IV). To radiolabel newly synthesized proteins, cultured monolayers of bovine anterior lens capsule epithelial (ALCE) cells were pulsed with [(3)H] proline. To identify proteins, an immunofluorescent technique, immunoprecipitation with specific antibodies, and electrophoretic separation on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were used. DEX increased production of total proteins, whereas it specifically decreased synthesis of FN and CN IV. A decrease in FN and CN IV synthesis by DEX in ALCE cells may decrease adhesion of lens epithelium to the basement membrane (BM), which may in turn cause pathogenesis. Messenger RNAs were identified by Northern blot analyses using specific DNA probes. Treatment of lens epithelial cells with DEX causes a 100-150% up-regulation of SPARC mRNA in a concentration-dependent fashion. The increase in the expression of FN mRNA by DEX was in a dose-response fashion and varied from 50-600%. A 24-hour treatment with DEX (10(-6)M) increased CN IV mRNA levels to 386% over baseline levels. Thus showing a differential upregulation by DEX of mRNAs of SPARC, FN, and CN IV. Results of nuclear run-on transcription assays indicate that regulation of RNAs by DEX may occur, in part, at the transcriptional level. The aberrant expression of lens basement membrane proteins by DEX may contribute to abnormal lens cell function and ultimately to anterior subcapsular cataract.

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In Vitro Production of Steroid Cataract in Bovine Lens

Cited by 13 publications

References 6 publications

In Vitro Production of Steroid Cataract in Bovine Lens

In Vitro Production of Steroid Cataract in Bovine Lens

Etiopathogenesis of cataract: An appraisal

Expression and Regulation of Sparc, Fibronectin, and Collagen Iv by Dexamethasone in Lens Epithelial Cells

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