In vitro propagation and acclimatization of genipapo accessions

Abstract

Genipapo is a species with an intermediate seed storage behavior, and its germination is slow and asynchronous; therefore the tissue culture is an alternative strategy for the propagation in large-scale. This study aimed to evaluate three genipapo accessions at different micropropagation stages (adventitious shoot induction, in vitro rooting and acclimatization). Genipapo nodal segments from the Sabinópolis (AS), Cruz das Almas (CZA) and Núcleo Bandeirante (NB) accessions were used and inoculated in culture media with 0 and 1.0 mg L-1 6-benzylaminopurine (BAP). Different indole-3-butyric acid (IBA) concentrations were tested for in vitro rooting. Subsequently, the effect of two substrates on the seedling development of three genipapo accessions was assessed. The molecular characterization of accessions was performed using 15 inter-simple sequence repeat (ISSR) primers, and genotype clustering was performed based on genetic dissimilarity using the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA). Culture media supplementation with 1.0 mg L-1 BAP favors the development of adventitious shoots and promotes a higher callogenesis percentage, leaf number and feasibility at 90 days of in vitro culture. The immersion of the basal portion of shoots for 20 seconds in all IBA solutions induces the rhizogenesis in the genipapo accessions at 90 days of in vitro culture. The substrates consisting of washed sand + dried coconut shell powder, at a 1:1 ratio by volume, and Topstrato HT(r) may be recommended for genipapo acclimatization. The NB accession is genetically different from the CZA and AS populations and shows superiority for most of the variables analyzed.

In vitro propagation of Campomanesia rufa: An endangered fruit species

“…Similar results were observed for number of leaves in which the referred treatment provided the formation of 9.5 leaves/ explant when such explants were cultured in medium supplemented with 1 μM BAP under conventional white light ( Figure 3C). As also shown in the present study, BAP has been widely used in in vitro culture of woody species due to its efficiency in the induction of adventitious buds and leaves (Prudente et al, 2016;Sa et al, 2016).…”

Conservação ex situ e caracterização morfoagronômica de germoplasma de jenipapeiro

“…Foram 21 artigos científicos, um livro, dois capítulos de livro e três publicações técnicas, além de seis dissertações e três teses. Os principais temas abordados foram cultura de tecidos/micropropagação (42,30%) [14][15][16][17][18][19][30][31][32][33]; diversidade genética/biologia molecular (11,53%) [34][35][36]; recursos genéticos (15,38%) [4,12,[37][38][39]; bioativos (11,53%) [20,21,40]; propagação (7,69%) [13,41] e pós-colheita (11,53%) [42][43][44] Os estudos sobre diversidade têm mostrado a necessidade de estabelecer estratégias que permitam a recuperação e o aumento da diversidade genética tanto in situ quanto ex situ. Os resultados da cultura de tecidos têm avançado e a expectativa é ter alguns acessos do BAG mantidos in vitro.…”

“…A diversidade genética desse BAG foi avaliada pela primeira vez por Silva et al (2014) [13], sendo que o banco continha, na época, 160 genótipos. No BAG também tem sido desenvolvidos protocolos para micropropagação, criopreservação e embriogênese somática [14][15][16][17][18][19], e a avaliação de quimiodiversidade e bioatividade do óleo essencial, no qual se verificou que os compostos do óleo essencial e do extrato metanólico de folhas de acessos possuem potencial para auxiliar no controle do ácaro-da-necrose-do-coqueiro [Aceria guerreronis (Acari: Eryophyidae)] [20,21].…”

Conservation, Utilization, Genetic and Chemodiversity of Germplasm of Genipap (Genipa Americana L.) in Brazil

“…The molecular characterization of accessions was performed using 15 inter-simple sequence repeat (ISSR) primers, and genotype clustering was performed based on genetic dissimilarity using the Unweighted Pair-Group Method with Arithmetic Mean (UPGMA). The NB (Núcleo Bandeirante) accession is genetically different from the CZA (Cruz das Almas) and AS (Sabinópolis) populations and shows superiority for most of the variables analyzed [26]. In Sergipe state, in the lower course of São Francisco River, the genetic diversity among 18 individuals of G. americana by RAPD markers using 12 primers, was evaluate.…”

“…best strategy is used the filed collections, not being indicated conservation way seed bank [4,26]. On the other hand, the application of techniques of tissue culture is important for species such as genipap to overcome the difficulties imposed by the traditional propagation system, enabling large-scale multiplication and the conservation of this species with holds good prospect for the future (Figure 3) [35][36][37].…”