In vitro propagation and synseed mediated short-term conservation of Scutellaria alpina L. and Scutellaria altissima L.

Zakaria, Ibrahim Alhassan; Sherman, Samantha H; Vaidya, Brajesh Nanda; Joshee, Nirmal

doi:10.46526/pccm.2020.v16.163

Cited by 2 publications

(2 citation statements)

References 41 publications

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“…In the in vitro rooting stage, using ¼ strength MS medium supplemented with 15 g/l sucrose + 2.0 g/l AC + 0.5 mg/l NAA increased root number and dry weight. Similar results were obtained by Demiralay et al (1998) on Ficus carica, and by Zakaria et al (2020) on Scutellaria Alpina and S. altissima. When AC was totally eliminated from the in vitro growing medium only shoot length was positively affected.…”

Section: Discussionsupporting

confidence: 85%

A Comprehensive Protocol for In Vitro Propagation and Acclimatization of Jacaranda mimosifolia Trees under the Egyptian Conditions

Sabah,

Hosni,

Abdul Raziq

et al. 2023

Egyptian Journal of Agricultural Sciences

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This study was carried out during the autumn season of 2021-2022 to establish a detailed protocol for the micropropagation of Jacaranda mimosifolia trees. The best results in terms of eliminating contamination (0.0%) were obtained by soaking seeds in Clorox (NaOCl) solution for 9 or 12 min. The highest seeds survival rate (100%) was obtained when sterile seeds were planted in ¾ strength MS medium + 30 g/l sucrose + 6% agar, and pH 5.7. The results showed that ½ strength MS was a suitable medium for the next multiplication stage, because it later gave the highest number of shoots, and chlorophyll A content. On the other hand, the ¼ strength MS medium was suitable for the rooting stage because it resulted in the best root length and shoot length. In terms of shoot length and fresh weight were obtained with 2.0 mg/l 6-BAP + 0.0 mg/l NAA, while 4.0 mg/l 6-BAP + 0.0 mg/l NAA gave the highest number of shoots, and 1.0 mg/l 6-BAP + 0.0 mg/l NAA resulted in the best flavonoids content. The highest root number and dry weight were obtained with 2.0 g/l AC + 0.5 mg/l NAA, while the longest shoots were obtained with 0.0 g/l AC + 0.1 mg/l NAA. In the acclimatization stage, the best plant height was obtained with Peat moss + Perlite at a ratio of 2:1 (v/v). The survival percentage (100%) was equal in both of the tested acclimatization media, viz., Peat moss + perlite at 2:1 (v/v) and Peat moss + perlite + vermiculite at 2:1:1 (v/v/v). The economic feasibility and production costs were calculated after determining the appropriate media for the different micropropagation stages of J. mimosifolia.

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Section: Discussionsupporting

confidence: 85%

A Comprehensive Protocol for In Vitro Propagation and Acclimatization of Jacaranda mimosifolia Trees under the Egyptian Conditions

Sabah,

Hosni,

Abdul Raziq

et al. 2023

Egyptian Journal of Agricultural Sciences

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“…These techniques also contribute to the conservation of endangered and endemic medicinal plant species [13]. So far, research on the in vitro micropropagation of the Scutellaria genus has focused on species, such as S. orientalis [14,15], S. alpine [16,17], S. araxiensis [18], S. barbata, and S. racemosa [19]. They indicate the possibility of regeneration by both indirect and direct organogenesis.…”

Section: Introductionmentioning

confidence: 99%

The Influence of Cytokinin on the Multiplication Efficiency and Genetic Stability of Scutellaria baicalensis Regenerants in In Vitro Culture Conditions

Dyduch-Siemińska,

Gawroński

2024

Applied Sciences

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The efficiency and method of regeneration in in vitro culture conditions depend primarily on the plant growth regulators (PGRs) used. Even growth regulators belonging to one group may have different effects, stimulating the process of direct or indirect organogenesis, thus possibly disturbing the genetic stability among regenerants. The main aim of this study was to identify the genetic stability of Scutellaria baicalensis regenerates obtained by in vitro culture method using start codon targeted (ScoT) markers. S.baicalensis nodal explants were regenerated on MS medium supplemented with kinetin (KIN) at concentrations of 0.25, 0.5, 1.0, and 2.0 mg × dm−3 or benzylaminopurine (BAP)—0.25, 0.5, 1.0, and 2.0 mg × dm−3. The effects of the number of propagated shoots, length, number of nodes, and fresh mass of regenerants were assessed. Moreover, the genetic stability of the regenerants was analyzed using start codon targeted (SCoT) markers. Direct shoot organogenesis was observed on an MS medium containing kinetin, while indirect shoot induction occurred on an MS medium supplemented with BAP. The highest average number of shoots (3.6) was achieved for the MS + KIN medium at a concentration of 0.25 and 5.8 for the MS + BAP 1.0 medium. The average length and average number of nodes were the highest on the MS + BAP 0.25 medium (50.0 and 6.0, respectively), while the lowest values of these features were observed on the MS + KIN 2.0 medium (40.3 and 4.9, respectively). A total of 111 amplified bands were exhibited by SCoT primers. Three of the analyzed primers revealed four unique genotype-specific markers. The average percentage of polymorphism obtained was 36.7%. The analysis of genetic similarity revealed a high level of genetic similarity between the donor plant and regenerants obtained on MS “0” (medium without the addition of phytohormones). A slightly lower value of genetic similarity was observed for regenerants obtained by direct organogenesis (MS + KIN medium at all concentrations). Indirect shoot organogenesis observed on the MS + BAP medium (all concentrations) resulted in the highest differentiation, both in relation to the donor plant and MS “0” regenerants. The results of our work indicate that, in the case of S. baicalensis, the maintenance of genetic stability depends primarily on the presence of the cytokinin type in the medium.

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In vitro propagation and synseed mediated short-term conservation of Scutellaria alpina L. and Scutellaria altissima L.

Abstract: In vitro propagation and synseed mediated short-term conservation of Scutellaria alpina L. and Scutellaria altissima L.

Cited by 2 publications

References 41 publications

A Comprehensive Protocol for In Vitro Propagation and Acclimatization of Jacaranda mimosifolia Trees under the Egyptian Conditions

A Comprehensive Protocol for In Vitro Propagation and Acclimatization of Jacaranda mimosifolia Trees under the Egyptian Conditions

The Influence of Cytokinin on the Multiplication Efficiency and Genetic Stability of Scutellaria baicalensis Regenerants in In Vitro Culture Conditions

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