In vitro propagation from axillary buds of the endangered tree &lt;i&gt;Dalbergia congestiflora&lt;/i&gt; Pittier (Fabaceae)

Hernández-García, Alejandra; Parra, Enrique Ambriz; López-Albarrán, Pablo; León, José Cruz de; Salgado‐Garciglia, Rafael

doi:10.5511/plantbiotechnology.21.0901a

Cited by 3 publications

(2 citation statements)

References 31 publications

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“…As in HIRAKAWA & TANNO, 2022, cytokinins inhibited axillary bud formation and bud development. HERNANDEZ-GARCIA et al (2021) also suggested that shoot regeneration was significantly lower in treatments with higher BAP concentrations (>1.0 mg/L). Moreover, LI et al (2008) showed that generally high-concentration of auxin and lowconcentration of cytokinin were beneficial to callus induction.…”

Section: Resultsmentioning

confidence: 93%

“…In general, IBA was the most commonly used auxin for rooting in different apple genotypes, and rooting percentage varied from 18 to 100% (TEIXEIRA DA SILVA et al, 2019). IBA is the most common auxin to induce root formation because it is more potent than IAA or synthetic auxins (HERNANDEZ-GARCIA et al, 2021). In addition, there was basically no callus formation at the bottom of the buds, accompanied by many fibrous roots (Figure 1D and F).…”

Section: Resultsmentioning

confidence: 98%

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Optimization of ‘Zi Dieer’ crabapple micropropagation through proliferation of axillary shoots

Li,

Zhou,

Zhang

et al. 2024

Cienc. Rural

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Malus is an important ornamental plant around the world and widely used in commercial production due to its diversity and wide adaptability. ‘Zi Dieer’ crabapple has good adaptability to a wide temperature range (high and low), disease resistance, and high commercial value. To satisfy the market demand, we established ‘Zi Dieer’ crabapple micro-regeneration system for large-scale reproduction. Induced buds were cultured on Murashige and Skoog (MS) medium supplemented with various concentrations of 6-benzylaminopurine (BAP: 0.5, 1.0, 1.5 mg/L) and 1-naphthaleneacetic acid (NAA: 0.05, 0.1, 0.3 mg/L) for 35 days. The highest bud induction rate (93.3%) was observed on MS medium supplemented with 1.0 mg/L BAP and 0.1 mg/L NAA, with average micro-shoot length of 1.32 cm. The germinated buds (1 cm in length) were propagated and cultured on MS medium supplemented with BAP (1.0, 1.5, 2.0 mg/L) and NAA (0.1, 0.3, 0.5 mg/L), after 35 days, on the MS basal medium supplemented with 1.5 mg/L BAP and 0.3 mg/L NAA, the best average number of new shoots is 8.56±0.13 (P<0.05) and good shoot length (4.94±0.09 cm), reproduction coefficient higher than MS medium (0) without PGRs. In order to induce rooting, the shoots (length 2 cm) are inserted in half strength MS (1/2 MS) medium containing 0.1, 0.3 and 0.5 mg/L of 3-indole butyric acid (IBA) or NAA, and 1 g/L activated carbon (AC) was added on the basis of NAA 0.3 mg/L to study the effect of activated carbon on the rooting of crabapple. After 38 days of culture, compared with other treatments, among the explants treated with IBA, the rooting rate was higher in the 1/2 MS medium containing 0.1 mg/L (82.1%), and the highest root number (11.29±0.52) was observed, and the root length was the other treatments 2-3 times. Although, the highest rooting rate was observed on 1/2 MS medium containing 0.1mg/L NAA (87.8 %), but the bottom of these buds formed large callus, which was not conducive to survival, and the root length was short. In summary, a feasible and complete rapid propagation program for ‘Zi Dieer’ crabapple has been developed.

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Section: Resultsmentioning

confidence: 93%

Section: Resultsmentioning

confidence: 98%

Optimization of ‘Zi Dieer’ crabapple micropropagation through proliferation of axillary shoots

Li,

Zhou,

Zhang

et al. 2024

Cienc. Rural

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In Vitro Propagation and Conservation of Lavandula stoechas subsp. luisieri and Pterospartum tridentatum, Two Important Medicinal and Aromatic Species from Portugal

Domingues,

Eira,

Ramalho

et al. 2024

Plants

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Lavandula stoechas subsp. luisieri and Pterospartum tridentatum are two valuable aromatic and medicinal plants. Their biometric and morphological parameters, such as the number of new shoots, length of the longest shoot, multiplication rate, and fresh weight, were evaluated using the multiplication MS medium protocol. The rooting protocols involved immersing the explants in IBA (1 g L−1) and a commercial IBA (3.3 g L−1) preparation (Clonex®). Slow-growth conservation assays were carried out using two different sucrose concentrations (15 g L−1 and 30 g L−1), and a control, with the cultures kept at 4 °C for 12 months. The multiplication rate for L. stoechas subsp. luisieri was 6.8, and that of P. tridentatum was 13.3, achieved using the MS medium supplemented with 0.2 mg L−1 BAP, 1 mg L−1 BAP, and 0.5 mg L−1 IBA. The application of Clonex® showed the best ex vitro rooting results in L. stoechas subsp. luisieri (77%) and P. tridentatum (90%). In the slow-growth conservation assays, at 4 °C, in darkness for 12 months, an excellent survival rate was achieved in L. stoechas subsp. luisieri (>80%) and P. tridentatum (>90%), even at the reduced sucrose concentration. This study demonstrates the effectiveness of in vitro multiplication and ex vitro rooting protocols for two valuable aromatic and medicinal plants. These findings are significant for the ex situ conservation of these species, as they provide effective long-term preservation and utilization strategies.

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From the Editors

Aoki

2022

Plant Biotechnology

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In vitro propagation from axillary buds of the endangered tree <i>Dalbergia congestiflora</i> Pittier (Fabaceae)

Cited by 3 publications

References 31 publications

Optimization of ‘Zi Dieer’ crabapple micropropagation through proliferation of axillary shoots

Optimization of ‘Zi Dieer’ crabapple micropropagation through proliferation of axillary shoots

In Vitro Propagation and Conservation of Lavandula stoechas subsp. luisieri and Pterospartum tridentatum, Two Important Medicinal and Aromatic Species from Portugal

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