In Vitro Propagation of Akama Tulip via Adventitious Organogenesis From Bulb Slices

Minas, G.J.

doi:10.17660/actahortic.2007.755.40

Cited by 4 publications

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“…The morphogenic response of the explants depends on the presence and concentration of growth regulators in the medium (Gaspar et al 2003 ). To initiate organogenesis in tulips, BAP is usually used, either alone or with auxin (Wright and Alderson 1980 ; Taeb and Alderson 1990 ; Minas 2007 ), although Famelaer et al ( 1996 ) used only picloram and 2,4-dichlorophenoxyacetic acid (2,4-D) to obtain callus tissue. In this study, only 0.5 μM BAP was used to stimulate organogenesis.…”

Section: Discussionmentioning

confidence: 99%

Induction of bulb organogenesis in in vitro cultures of tarda tulip (Tulipa tarda Stapf.) from seed-derived explants

Maslanka

Bach

2014

In Vitro Cell.Dev.Biol.-Plant

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A protocol for obtaining bulbs via in vitro organogenesis was developed for tarda tulip (Tulipa tarda Stapf). Scale explants were obtained from bulbs formed at the base of seedlings or from adventitious bulbs that developed from callus tissue forming on stolons or on germinating seeds. Some explants were subjected to chilling at 5°C for 12 wk. The culture media contained 3 or 6% sucrose and was supplemented with either no growth regulators, either 0.5 μM 6-benzyl-aminopurine (BAP) or 18.9 or 94.6 μM abscisic acid (ABA). Cultures were maintained in the dark at 20°C. Callus tissue developed mainly on media without growth regulators or with BAP. Callus was formed from up to 96% of explants derived from non-chilled adventitious bulbs that were treated with 3% sucrose and 0.5 μM BAP. Less callus was formed from chilled explants compared with non-chilled explants. Newly formed adventitious bulbs appeared on the explants via direct and indirect organogenesis. The media with BAP promoted the formation of adventitious bulbs at a rate of 56–92% from non-chilled explants, whereas a maximum rate of 36% was observed from chilled explants. ABA inhibited the induction of adventitious bulbs and callus. The adventitious bulbs obtained in these experiments contained a meristem, which was evidence that they had developed properly.

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Section: Discussionmentioning

confidence: 99%

Induction of bulb organogenesis in in vitro cultures of tarda tulip (Tulipa tarda Stapf.) from seed-derived explants

Maslanka

Bach

2014

In Vitro Cell.Dev.Biol.-Plant

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“…Sexual reproduction guarantees the maintenance and broadening of the genetic pool but the transition from seed to a flowering bulb can take up to seven years (Minas 2007;Shahin et al 2012a). The seedling, which is at the juvenile stage needs to form a small bulb during the first growth cycle and thereafter, the bulb will increase its biomass in every growth cycle until it reaches the reproductive stage (Le Nard and de Hertogh 1993).…”

Section: Discussionmentioning

confidence: 99%

“…Tulip and lily can sexually propagate through seeds but once they sprout, their juvenile and adult vegetative stage can last three to seven years, depending on the genotype and growth conditions, until they finally transition to the reproductive stage (Minas 2007;Shahin et al 2012). …”

Section: What Is Known About Vegetative Propagation In Tulip and Lily?mentioning

confidence: 99%

“…Due to the low number of daughter bulbs obtained by axillary bud outgrowth in tulip and the long sexual reproduction cycle, several investigations aiming to improve its regeneration capacity through in vitro culture have been made over the last fifty years Alderson and Taeb 1990;Baker and Wilkins 1988;Hulscher et al 1992; Nishiuchi and Koster 1988 ;Podwyszyńska 2005;Podwyszyńska 2006;Podwyszynska and Sochacki 2010;Taeb and Alderson 1990a, b;Minas 2007;van Rossum 1997). All those studies adopted a physiological approach by testing the regeneration response to a plethora of exogenous supplied treatments and cues, including hormones, antioxidants, and different temperature regimes.…”

Section: Artificial Propagation In Tulip Bulbsmentioning

confidence: 99%

“…There are two bottleneck aspects during in vitro regeneration in tulip. First, the limited amount of competent tissues and second, that regeneration results in shoots and not in bulbs (Flavell 2009;Alderson and Rice 1983;Alderson and Taeb 1990;Baker and Wilkins 1988;Maślanka and Bach 2014;Minas 2007;Podwyszyńska 2006;Podwyszynska and Sochacki 2010;Taeb and Alderson 1990b). Young floral stalks are the most commonly used explant in tulip because they bypass the callus stage and regenerate adventitious shoots under shoot induction medium (Alderson and Taeb 1990).…”

Section: Lily Bulb Scales As a Model To Study Regeneration Recalcitramentioning

confidence: 99%

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Mechanisms of vegetative propagation in bulbs : a molecular approach

Moreno-Pachon

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Role of Tulipa gesneriana TEOSINTE BRANCHED1 (TgTB1) in the control of axillary bud outgrowth in bulbs

et al. 2017

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Key messageTulip vegetative reproduction.AbstractTulips reproduce asexually by the outgrowth of their axillary meristems located in the axil of each bulb scale. The number of axillary meristems in one bulb is low, and not all of them grow out during the yearly growth cycle of the bulb. Since the degree of axillary bud outgrowth in tulip determines the success of their vegetative propagation, this study aimed at understanding the mechanism controlling the differential axillary bud activity. We used a combined physiological and “bottom-up” molecular approach to shed light on this process and found that first two inner located buds do not seem to experience dormancy during the growth cycle, while mid-located buds enter dormancy by the end of the growing season. Dormancy was assessed by weight increase and TgTB1 expression levels, a conserved TCP transcription factor and well-known master integrator of environmental and endogenous signals influencing axillary meristem outgrowth in plants. We showed that TgTB1 expression in tulip bulbs can be modulated by sucrose, cytokinin and strigolactone, just as it has been reported for other species. However, the limited growth of mid-located buds, even when their TgTB1 expression is downregulated, points at other factors, probably physical, inhibiting their growth. We conclude that the time of axillary bud initiation determines the degree of dormancy and the sink strength of the bud. Thus, development, apical dominance, sink strength, hormonal cross-talk, expression of TgTB1 and other possibly physical but unidentified players, all converge to determine the growth capacity of tulip axillary buds.Electronic supplementary materialThe online version of this article (10.1007/s00497-017-0316-z) contains supplementary material, which is available to authorized users.

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In Vitro Propagation of Akama Tulip via Adventitious Organogenesis From Bulb Slices

Cited by 4 publications

References 0 publications

Induction of bulb organogenesis in in vitro cultures of tarda tulip (Tulipa tarda Stapf.) from seed-derived explants

Induction of bulb organogenesis in in vitro cultures of tarda tulip (Tulipa tarda Stapf.) from seed-derived explants

Mechanisms of vegetative propagation in bulbs : a molecular approach

Role of Tulipa gesneriana TEOSINTE BRANCHED1 (TgTB1) in the control of axillary bud outgrowth in bulbs

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