In Vitro Propagation of an Edible Bamboo Bam-busa Bambos and Assessment of Clonal Fidelity through Molecular Markers

Abstract

 Abstract-An efficient and reproducible protocol has been established through the technique of forced axillary branching for the propagation of an important edible bamboo species namely Bambusa bambos. High frequency multiple shoot induction was achieved from nodal segments collected from elite genotype on Murashige and Skoog's (MS) medium supplemented with 4.4 µM Benzylaminopurine (BAP) and 1.16 µM Kinetin (Kn). The size of explant and season greatly influenced the frequency of bud break. Rooting posed a major problem to be worked out in this particular species. Best rooting response was observed on 9.80 µM of Indole-3 Butyric acid (IBA) with 60 ± 14.1 % rooting.In vitro raised plants were successfully acclimatized and established in the field conditions where they exhibited normal growth. In a bid to ascertain genetic fidelity, DNA was extracted by CTAB method and samples were analysed in 1.8% agarose gel electrophoresis. In the present study no variation was reported among the in vitro raised progeny and the mother plant in the banding profiles generated by the total of fifteen Random Amplified polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) markers. Hence, molecular analysis confirmed that these plants were genetically similar and can be used as elite plants.

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

“…In the present investigation, for in vitro shoot induction MS medium was used. The use of MS basal medium for micropropagation of B. bambos has been reported in earlier reports (Arya and Sharma, 1998;Nayak et al, 2010;Anand et al, 2013 andBrar, 2014). (Singh et al, 2000); B. edulis (Lin et al, 2004) and B. oldhamii (Lin et al, 2007).…”

“…Arya and Sharma, (1998); Arya and Arya, (2009) and Anand et al, (2013) reported 80-90% survival rate by using the same soil mixture in case of B. bambos. Devi and Sharma, (2009) used the similar potting mixture in case of Arundinaria callosa and reported 60-70% survival rate as well as Shood et al (2014) in Phyllostachys pubescens with 77% survival rate, Arya et al (2012) in D. hamiltonii, Mishra et al (2011) in B. tulda, Brar et al (2013) in D. membranaceus.…”

“…Similarly Arya and Sharma, (1998);Nayak et al, (2010); Anand et al, (2013) and Brar, (2014) also used nodal segments as explant to initiate the in vitro cultures of B. bambos. Most suitable result on surface sterilization was obtained when nodal segments of B. bambos were surface sterilized with 0.2% HgCl 2 for 7 min which resulted in 92% contamination free explants (Table 1).…”

“…(Singh et al, 2000); B. edulis (Lin et al, 2004) and B. oldhamii (Lin et al, 2007). Arya and Sharma, (1998);Anand et al, (2013) and Nayak et al, (2010) investigated that the cytokinins (BAP and Kn) were effective in direct shoot induction of the axillary buds of B. bambos as well as Devi andSharma, (2009) in Arundinaria callosa, andChaudhary et al, (2004) in D. strictus. BAP has also been found to be essential for direct shoot induction in bamboo (Nadgir et al, 1984;Banik, 1987 andSaxena, 1990).…”

Chemical Disinfestation for Sowing and in Vitro Re-cultivation of Orchids and its Influence on the Seedlings Nutrition

“…For small producers and collectors of orchids, these equipments are not readily available, due to the high cost. However, researchers have sought ways to simplify this in vitro cultivation technique such as the use of pressure cooker (Pierik 1997), microwave oven (Teixeira et al 2005;Venturieri et al 2013) and chemicals disinfectants for sterilization of culture medium (Anand et al 2013;Torres et al 2016;Ray et al 2017). …”