In vitro propagation of Anacardium occidentale L.

D’Silva, Icy; D’Souza, Lancy

doi:10.1007/bf00036138

Cited by 30 publications

(3 citation statements)

References 13 publications

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“…In vitro rooting depends on the concentration of auxin, the quantity of mineral elements and sugar in the culture medium. AIB is one of the auxins most widely used for rooting young teak leafy shoots, often in combination with NAA [16]. For this study, different concentrations of AIB were tested on the in vitro rooting of teak on McCown Woody plant medium containing 60 g/l sucrose.…”

Section: Discussionmentioning

confidence: 99%

Effect of Cytokinins on Micropropagation of Teak (Tectona grandis L.) Grown in Cote D'ivoire

Stephane,

Éliane,

Hervé

et al. 2023

JABB

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Teak (Tectona grandis L.) is a timber tree highly prized on world markets for its stability and durability. This hardy species has good initial growth and is very important for the reforestation of Côte d'Ivoire. However, teak regeneration potential by seed or cutting still very weak. This situation is a major constraint to planting material production in quantity and quality. The aim of this study is to improve the production of teak seedlings for orchard renewal using in vitro culture methods. Mature teak seeds were surface disinfected and sown on Murashige and Skoog (MS) basal medium supplemented with different concentrations of Benzyl-aminopurine (BAP), Kinetin and Thidiazuron (TDZ). The shoot tip explants and nodal segments were taken from in vitro germination and placed on the same medium used for seeds germination. Leafy shoots obtained after four weeks of culture were excised and transferred individually on different strength of McCown Woody plant medium supplemented with various concentrations of AIB for rhizogenesis. Teak seeds germination started seven days after sowing. BAP and Kinetin exhibited highest percentage of normal seedlings (83.46% and 74.14% respectively). Higher seedlings (7.48 cm) were obtained on medium supplemented with 0.5 mg/L kinetin. The shortest bud break time (7.43 days) was observed on medium containing 0.5 mg/L BAP. Leafy shoot production was optimal (5 leafy shoots per explant) with the nodal segment on culture medium containing 2 mg/l BAP. Half strength of McCown Woody plant medium containing 60 g/L sucrose and 4 mg/l AIB induced the highest rooting percentage (73%) and roots number per plant (03) natural environment.

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Section: Discussionmentioning

confidence: 99%

Effect of Cytokinins on Micropropagation of Teak (Tectona grandis L.) Grown in Cote D'ivoire

Stephane,

Éliane,

Hervé

et al. 2023

JABB

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“…Work on cashew tissue culture has been in progress on developing multiplication protocols from cashew explants. However, regeneration protocols from mature explants are yet to be developed, although, reports are available on multiplication and field establishment of cashew regenerated from young nodal cuttings (Philip, 1984;D'Silva and D'Souza, 1992;Lievens et al, 1989;Keshavachandran and Khader, 1990;Leva and Falcone, 1990;Nair and Mohanakumar, 1993;Das et al, 1996;Thimmappaiah and Shirly, 1999). Somatic embryogenesis was induced from nucellar tissue cultured from 2-3 week old immature nuts.…”

Section: Biotechnology In Cashewmentioning

confidence: 99%

Cashew research in India

Bhat,

Nagaraja,

Rupa

2010

J. Hortic. Sci.

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Cashew, after its introduction from Brazil during the 16th Century, has established very well in India. A total of 40 high-yielding varieties have been released so far by the Directorate of Cashew Research, Puttur, and various Agricultural Universities, for cultivation. Of these, 13 are hybrids and 27 are selections. Research achievements in the area of crop improvement, management, protection and post-harvest technology over the last six decades are reviewed and documented here. As India has been importing raw nuts to the tune of 6.5 lakh tons annually to cater the demand of established processing factories, research priorities have been identified to meet the challenges of enhancing production and productivity of cashew in the country.

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“…In efforts to develop a micropropagation protocol for cashew, a complete regeneration system has been obtained through organogenesis (Philip, 1984;Lievens et al, 1989;Falcone and Leva, 1990;Hegde et al, 1991;D'Silva and D'Souza, 1992;Das et al, 1996;Boggetti et al, 1999;Thimmappaiah and Samuel, 1999). However, attempts to formulate regeneration pathways by somatic embryogenesis have met with limited success (Jha, 1988;Lakshmi Sita, 1989;Sy and Martinelli, 1991;Hegde et al, 1994;Ananthakrishnan et al, 1999).…”

Section: Introductionmentioning

confidence: 98%

Induction of somatic embryogenesis in cashewnut (Anacardium occidentale L.)

Gogte

Nadgauda

2000

In Vitro Cell.Dev.Biol.-Plant

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Somatic embryogenesis was induced in callus cultures derived from nucellar tissue of cashewnut (Anacardium occidentale L.). Callus was obtained from nucellar tissue after 3 wk of culture on semisolid Murashige and Skoog (MS) basal medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D, 5 mM) ϩ gibberellic acid (GA 3 , 15 mM) ϩ N 6 -benzyladenine (BA, 5 mM). This callus gave rise to an embryogenic mass after 9 wk on maintenance medium containing 2, 4-D (10 mM) ϩ GA 3 (15 mM) ϩ 4% sucrose ϩ 0.5% activated charcoal ϩ 10% coconut water (CW) ϩ 0.05% casein hydrolysate (CH). The embryogenic mass, after transfer to medium supplemented with 2,4-D (5 mM) ϩ GA 3 (30 mM) ϩ 4% sucrose ϩ 0.5% activated charcoal ϩ 10% CW ϩ 0.05% CH, gave rise to somatic embryos. The developmental stages of somatic embryos were observed using light and stereo microscopes. Histological study of somatic embryo development was also carried out. The present study would be useful for clonal propagation, and variety improvement in cashewnut, which is essential due to its increasing demand and export potential.

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In vitro propagation of Anacardium occidentale L.

Cited by 30 publications

References 13 publications

Effect of Cytokinins on Micropropagation of Teak (Tectona grandis L.) Grown in Cote D'ivoire

Effect of Cytokinins on Micropropagation of Teak (Tectona grandis L.) Grown in Cote D'ivoire

Cashew research in India

Induction of somatic embryogenesis in cashewnut (Anacardium occidentale L.)

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