In vitro propagation of Caralluma tuberculata and evaluation of antioxidant potential

Rehman, Riaz Ur; Chaudhary, Muhammad Fayyaz; Khawar, Khalid Mahmood; Lü, Gang; Mannan, Abdul; Zia, Muhammad

doi:10.2478/s11756-013-0322-z

Cited by 41 publications

(34 citation statements)

References 48 publications

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“…The ethanolic extract of the plant showed moderate nitric oxide scavenging activity at a concentration of 60 mg when compared to gallic acid as a standard. Rehman et al [46] reported the antioxidant properties of different extracts of C. tuberculate. Jerome et al [47] reported the phytochemical constituents in C. umbellate, also suggesting that this genus has high pharmacological potential.…”

Section: Free-radical-scavenging Activitiesmentioning

confidence: 99%

Free-radical-scavenging and antioxidant capacities with special emphasis on enzyme activities andin vitrostudies inCaralluma flavaN. E. Br.

Karthishwaran

Shamisi

Kurup

et al. 2017

Biotechnology & Biotechnological Equipment

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Caralluma flava is a perennial, succulent edible plant distributed in the hillsides of the United Arab Emirates. In order to validate its use in the traditional system as an edible and medicinal source, we evaluated the antioxidant and in vitro free-radical-scavenging properties of its ethanol extract. The antioxidant activity of the extract was evaluated in vitro by 2,2'-azino-bis-3-ethylbenzothiazoline-6sulfonic acid (ABTS + ), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), hydroxyl radical and nitric oxide radical scavenging assays. The antioxidant enzyme activities (superoxide dismutase, catalase and glutathione peroxidase) and non-enzymatic antioxidant contents (total reduced glutathione, and Vitamin E and Vitamin C) in the extracts were also determined. The total phenolics content of the plant extract was 10.14 mg/g and the flavonoid content was 4.13 mg/g. The results showed a positive linear correlation between these phytochemicals and the free-radical-scavenging activities. The C. flava extract exhibited significant radical-scavenging properties in the ABTS( + ), DPPH( ), hydroxyl radical and nitric oxide radical assays. The study revealed that C. flava could be considered an excellent source of enzymatic and non-enzymatic antioxidants. The results indicate that the plant extract has potent free-radical-scavenging activity and ability to prevent radical chain reactions. Based on the results obtained, C. flava seems to be a very promising species for further investigation in order to identify the compounds responsible for its biological activity.

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Section: Free-radical-scavenging Activitiesmentioning

confidence: 99%

Free-radical-scavenging and antioxidant capacities with special emphasis on enzyme activities andin vitrostudies inCaralluma flavaN. E. Br.

Karthishwaran

Shamisi

Kurup

et al. 2017

Biotechnology & Biotechnological Equipment

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“…Nikam et al (2007) reported the highest percentage of rooting, number of roots and root length in C. hirsuta, when the nodal explants derived in vitro shoots were cultured on the half strength MS medium fortified with 2.0 µM IAA. When the microshoots of Caralluma tu berculata were cultured on half strength MS medium supple mented with 5.07 µM IAA, they were produced 3.0±0.15 roots/microshoot (Rehman et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Plant regeneration from cotyledon and leaf explants of Ceropegia bulbosa Roxb., an endangered medicinal plant

Subbaiyan¹,

Thangapandian²

2017

Bot Pac

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An efficient protocol of callus induction, shoot multiplication and plant rege ne ra tion from cotyledon and leaf explants of Ceropegia bulbosa has been elaborated. The highest percentage (90.66) of callus induction was observed when cotyle do nary explants cultured on MS medium having 0.5 mg/L 2,4D in combination with 0.05 mg/L Kn followed by 86.25 % callus induction from leaf explant at 0.5 mg/l BAP in combination with 0.05 mg/l Kn. Shoot multiplication was ob served, cotyledon derived callus cultured on 1.0 mg/L BAP in combination with 0.10 mg/L NAA could induce number of shoots (4.71) and their lengths (6.13 cm). Cotyledonary callus derived microshoots gave the highest rooting percen tage (92.26 %) root numbers (13.14) and their lengths (8.87 cm) were observed on halfstrength MS medium fortified with 0.4 mg/L IBA. In vitro derived plant lets were hardened in the greenhouse and successfully established into the soil. Переведено редакцией.

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“…Caralluma species have been used by humans as tonic, carminative, anti-helminthic and anti-tumor agent. 8 Caralluma is reported to be effective in rheumatism and also known to have hypotensive effect. 9,10 The phytochemical constituents include volatile alkaloids, cardiac glycosides, flavonoids, saponins, sterols and tannins.…”

Section: Caralluma Tuberculata Which Belongs To Familymentioning

confidence: 99%

Comparison of Caralluma tuberculata with Metformin for Anti-Diabetic Activity: An Animal Study

Faisal

Hayat

Inayat

et al. 2019

JIMDC

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Background: Caralluma tuberculata, is a wild herb, which grows in the hills of Balochistan and has been known to have medicinal and nutritional properties since antiquity. This experimental research was designed to study the hypoglycemic properties of Caralluma tuberculata and to compare them with Metformin.Material and Methods: This was a laboratory-based animal experimental study. It was conducted in the Pharmacology laboratory of Khyber Medical University, Peshawar from February 2016 to August 2016. Two types of extracts of Caralluma tuberculata [crude extract and carbon tetrachloride (CCl4) extract] were prepared and administered to normal and alloxan treated diabetic rabbits. To study anti-diabetic activity, eighty-four rabbits were divided into two main groups. Group I (Normal/Non-Diabetic Rabbits; n= 21) and Group II (Diabetic/Alloxanized Rabbits; n=63). Each group was further divided into sub-groups (7 rabbits in each). Effect of Caralluma tuberculata, Metformin and 2% gum tragacanth on blood glucose levels were checked at 0, 2, 4, 6, 8, 12 and 24 hours of drug administration. The extracts were given in capsule form and in cooking oil. Data analysis was done using SPSS version 16. For calculation and comparison of the hypoglycemic effects at various doses and different time intervals, analysis of variance (ANOVA) and Tukey’s post hoc test were applied.Results: The crude extract, 200mg/kg body weight of Caralluma tuberculata showed significant decrease (p<0.001) in mean blood glucose levels from 2-hour till12 hours. Whereas, highly significant reduction of blood glucose was seen from 2 hours after treatment till 24 hours, when carbon tetrachloride (CCl4) fraction of Caralluma (100mg/kg body weight) in capsule form was administered. Metformin 500mg/kg body weight was given to compare its effects with plant crude extract and it was found that metformin appeared to be less effective in comparison with Caralluma tuberculata.Conclusion: Caralluma tuberculata lowered the blood glucose level and turned out to be more significant in developing hypoglycemia when taken with cooking oil. More work is essential to provide stronger evidence for the use of this natural agent in the management of Diabetes Mellites.

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In vitro propagation of Caralluma tuberculata and evaluation of antioxidant potential

Cited by 41 publications

References 48 publications

Free-radical-scavenging and antioxidant capacities with special emphasis on enzyme activities andin vitrostudies inCaralluma flavaN. E. Br.

Free-radical-scavenging and antioxidant capacities with special emphasis on enzyme activities andin vitrostudies inCaralluma flavaN. E. Br.

Plant regeneration from cotyledon and leaf explants of Ceropegia bulbosa Roxb., an endangered medicinal plant

Comparison of Caralluma tuberculata with Metformin for Anti-Diabetic Activity: An Animal Study

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