In vitro propagation of Cassia angustifolia through leaflet and cotyledon derived calli

Abstract

High efficiency shoot regeneration was achieved through leaflet and cotyledon derived calli in Cassia angustifolia -an important medicinal plant. Dark brown compact callus was induced at the cut ends of the explants on Murashige and Skoog's (MS) medium augmented with 1 μM N 6 -benzyladenine (BA) + 1 μM 2,4-dichlorophenoxyacetic acid (2,4-D). Such callus pieces on transfer to cytokinins (BA or kinetin) supplemented medium differentiated shoots within 10 -15 d. Of the two cytokinins, 5 μM BA was optimum for eliciting morphogenic response in 83.33 and 70.83 % cultures with an average of 4.16 ± 0.47 and 3.70 ± 0.56 shoots in cotyledon and leaflet derived calli, respectively. The addition of 0.5 μM α-naphthaleneacetic acid (NAA) to MS + 5 μM BA further elevated the maximum average number of shoots to 12.08 ± 1.04 and 5.37 ± 0.52 for cotyledon and leaflet calli, respectively. The excised shoots were transferred to a rooting medium containing either IAA (indole-3-acetic acid), IBA (indole-3-butyric acid) or NAA. Nearly 95 % shoots developed an average of 5.4 ± 0.41 roots on half strength MS medium supplemented with 10 μM IBA.

Influence of Plant Growth Regulators on In Vitro Shoot Multiplication and Plantlet Formation in Cassia angustifolia Vahl

“…The technique has been widely applied in all the sectors of horticulture, plantation and forestry and has contributed significantly towards the enhanced production of high quality planting material to the market. Some in vitro regeneration protocol are available for Cassia angustifolia through different explants by different methods, such as direct regeneration through cotyledonary node and nodal segments (Agrawal and Sardar 2003;Siddique and Anis 2007 a and b), indirect plant regeneration via cotyledon and leaflet derived calli (Agrawal and Sardar 2006;Siddique et al 2010) and somatic embryogenesis from cotyledon (Agrawal and Sardar 2007) but to the best of our knowledge, there is no report on direct organogenesis using shoot tip explants. Therefore, the objective of the present study was to compare the relative efficiency of shoot tip explants with previously available reports for in vitro micropropagation from different explants and to standardize the optimal concentration of cytokinin, or cytokinin and auxin combinations for maximum shoot production and establishment of complete plantlets.…”

Advances in Micropropagation of a Highly Important Cassia species- A Review

“…Different explants (Cotyledons, leaflets and petiole) excised from axenic seedlings were used for inducing organogenic callus. Agrawal and Sardar (2006) used cotyledons and leaflets for in vitro propagation. Cotyledons were more responsive where 91% cultures produced about 12 shoots per explant.…”

“…In cotyledons and leaflets explants, multiple shoots were observed when green, morphogenic callus (1.0 µM 2.4-D + 1.0 µM BA) was transferred to BA + NAA (Agrawal and Sardar 2006). In case of petiole, highest number of shoots and shoot length was recorded on MS medium along with 5.0 µM TDZ and 1.5 µM IAA (Siddique et al, 2010).…”

“…There is marked variation in the rooting potential of different plant species and systematic trials are often needed to define the conditions required for root induction. Agrawal and Sardar (2006) examined the effectiveness of various auxins on rooting of C. angustifolia microshoots and found that 10.0 µM IBA was superior to IAA or NAA. However 200 µM IBA was best for ex vitro rooting in C. angustifolia (Parveen and Shahzad 2011).…”

In vitro regenerative potentials of the medicinal plant Abutilon indicum (L.) Sweet

“…Over the last years, a number of micropropagative protocols have been developed using a variety of explants like shoot tip, axillary buds, stem cuttings and leaf segments. Callus mediated plant regeneration is an easy way to obtain somaclonal variants as has been emphasized in other several plants (Dhar and Joshi, 2005;Agrawal and Sardar, 2006). Moreover, in vitro morphogenesis without a callus phase is regarded as the most suitable strategy to obtain plants with high speed as well as genetical fidelity.…”