In vitro propagation of Dipterocarpus alatus and Dipterocarpus intricatus

Abstract

Seedlings were grown in vitro from embryos of Dipterocarpus alatus and D. intricatus.

The prospect of micropropagating Gonystylus bancanus (Miq.) Kurz, a tropical peat swamp forest timber species through tissue culture technique - Review

“…Earlier summary on tissue culture of some notable tropical timber species has been compiled and detailed out by Vaario (1996), where it was pointed out that the earliest tissue culture works on dipterocarps were reported by Smits and Struycken (1983) (Scott et al 1987), Shorea robusta Roth (Jain, Chaturvedi 1991), Dipterocarpus alatus Roxburgh and Dipterocarpus intricatus Dyer (Linington 1991), S. leprosula (Vaario et al 1993), and Vatica diospyroides Symington (Srisawat, Jongkraijak 2013) with various degree of success. It is interesting to note that some of the species such as D. alatus, D. intricatus (Linington 1991) and V. diospyroides (Srisawat, Jongkraijak 2013) are actually yet to show promising organogenic response from meristematic explant sources such as the shoot tips and nodal segment as compared to embryonic explants, even though the meristematic tissue used as explant was considered an area of active plant growth where undifferentiated cells divide and form new, specialized cells.…”

“…It is interesting to note that some of the species such as D. alatus, D. intricatus (Linington 1991) and V. diospyroides (Srisawat, Jongkraijak 2013) are actually yet to show promising organogenic response from meristematic explant sources such as the shoot tips and nodal segment as compared to embryonic explants, even though the meristematic tissue used as explant was considered an area of active plant growth where undifferentiated cells divide and form new, specialized cells. This evoked perception that tropical forest trees were indeed recalcitrant in nature and any attempt to manipulate their growth especially in vitro requires modification of protocols such as pre-treatment during sterilization and addition of a higher concentration of plant growth regulators to stimulate cell differentiation.…”

Oak somatic and gametic embryos maturation is affected by charcoal and specific aminoacids mixture

“…In vitro explants exude inhibitory phenolic compounds to the culture medium that may be adsorbed by activated charcoal (Fridborg et al, 1978), and its darkening effect may attenuate the activity of phenol oxidising enzymes (Linington, 1991). Also, activated charcoal absorbs 5-hydroxi-methyl-2-furaldehide (HMF), a secondary product of sucrose autoclaving which reduces plant growth (Rao and Pattabiraman, 1990).…”

A review of dipterocarps: taxonomy, ecology and silviculture

“…High resin content within the tissues may be at least partly responsible for this effect for some species. However, some success has been achieved by Smits and Struycken (1983), Scott et al (1988) and Linington (1991) in culturing the tissues of some Shorea and Dipterocarpus species.…”

“…Both contain information on silvical aspects. Research has been concentrating on collection, storage and germination of seeds and on mycorrhizae (e.g., Khemnark 1980, Panochit et al 1984, Panochit et al 1986, Chalermpongse 1987, Boontawee and Nutivijarn 1991, Linington 1991, Kantarli 1993. Concerning dipterocarp planting stock propagation the ASEAN Forest Tree Seed Centre concentrates on vegetative propagation (Mok 1994 Dipterocarpus alatus, D. costatus, D. intricatus, D. macrocarpus, D. obtusifolius, D. tuberculatus, Hopea ferrea, H. odorata, Shorea henryana, S. obtusa, S. roxburghii and S. siamensis. In Vietnam, some plantation work on an experimental scale is carried out in Dong Nai Province, in the Central Highlands and in Daklak Province (Doan 1985, Vu 1991, Dinh 1992.…”