In Vitro Propagation of Edithcolea grandis

Rose, Kemar; Williams, M. K.; Francis, Robert C.; Higgins, Shishca

doi:10.1007/s40011-023-01460-9

Cited by 3 publications

(1 citation statement)

References 17 publications

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“…The shoots were cultured in modi ed Murashige and Skoog (MS) (1962) media previously used in the in vitro propagation of another Asclepiadoideae species, Edithcolea grandis (Rose et al 2023). The pH was adjusted to 5.3 and the medium was autoclaved at 121°C and 15 psi for 20 minutes.…”

Section: Plant Materials and Initiation Of Culturementioning

confidence: 99%

Comparison of Three Cytokinins on In vitro Multiplication of Orbea semota, a Conical, Stout-Teethed Succulent

Rose,

Scantlebury,

Williams

et al. 2024

J Mod Agric Biotechnol

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Objective: Orbea semota (N.E. Br.) L.C. Leach is an erect or procumbent succulent with numerous branches. The stems of this plant are grey-green to dark green in color and possess flowers that are dark maroon or dark brown with yellow marks at the tips. It is native to Kenya, Tanzania and Rwanda and grows in rocky places. There is great potential for its use as a medicinal plant, also, there are immense prospects for commercial trade and cultivation of the succulent as an ornamental in Jamaica. The purpose of the study is to select the most effective cytokinin for the shoot multiplication of Orbea semota using stem cuttings. Methods: Young shoots were initiated for 6-8 weeks on a modified Murashige and Skoog (MS) medium. Initiated shoots were then cultivated on Media A, B, C and D for 8 weeks to promote shoot multiplication. These were modified MS media supplemented with three different cytokinins [6-γ, γ-Dimethylallylamino purine (2iP), 6-Benzylaminopurine (BA) and Kinetin] and a control without any cytokinins. Results: Medium A (containing 2iP) produced the best results at shoot multiplication than all other media used. The mean lengths of the shoots produced by Medium A were 19.0±11.4 mm and 10.5±3.8 mm. The mean number of shoots generated using Medium A were 3.4±1.3 and 4.0±1.9. These results were significantly greater than those of the shoots produced by all other media. Conclusion: The present study details an efficient and reproducible protocol for the in vitro shoot multiplication of Orbea semota. The results obtained showed that Medium A was the most optimal medium for promoting shoot multiplication. Therefore, of the three cytokinins investigated 2iP produced the best results at stimulating shoot multiplication and growth of Orbea semota.

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Section: Plant Materials and Initiation Of Culturementioning

confidence: 99%

Comparison of Three Cytokinins on In vitro Multiplication of Orbea semota, a Conical, Stout-Teethed Succulent

Rose,

Scantlebury,

Williams

et al. 2024

J Mod Agric Biotechnol

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An improved micropropagation protocol for the ex situ conservation of Thlaspi cariense A. Carlström (Brassicaceae): an endangered Turkish endemic plant species

Cüce,

Karaismailoğlu

2023

In Vitro Cell.Dev.Biol.-Plant

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Comparison of Three Cytokinins on In vitro Multiplication of Orbea semota, a Conical, Stout-Teethed Succulent

Rose,

Scantlebury,

Williams

et al. 2023

Preprint

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Background Orbea semota is a much-branched succulent with erect or procumbent grey-green to dark green stems with dark maroon or dark brown flowers possessing yellow marks at the tips. It is native to Kenya, Tanzania and Rwanda growing in rocky places and there is great potential for its use as a medicinal plant, as well as, commercial trade and cultivation as an ornamental in Jamaica. The purpose of the study is to select the most effective cytokinin for the shoot proliferation of Orbea semota using stem cuttings. Results Medium A (containing 2iP) produced the best results at shoot proliferation than all other media used. The mean lengths of the shoots produced by Medium A (19.0 ± 11.4 mm and 10.5 ± 3.8 mm) were significantly greater than that of the shoots produced by all other media. Significantly larger quantities of shoots were generated using Medium A than all other media (3.4 ± 1.3 and 4.0 ± 1.9). Conclusions The present study details an efficient and reproducible protocol for the in vitro shoot multiplication of Orbea semota. This current investigation assured effective establishment, mass multiplication and could offer an in vitro strategy for the ex situ conservation of this succulent that has great potential as a medicinal plant, as well as, commercial cultivation and trade as an ornamental in Jamaica. The results obtained showed that Medium A was the most optimal medium at promoting shoot proliferation. Therefore, of the three cytokinins investigated 2iP produced the best results at stimulating shoot multiplication and growth of Orbea semota.

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In Vitro Propagation of Edithcolea grandis

Cited by 3 publications

References 17 publications

Comparison of Three Cytokinins on In vitro Multiplication of Orbea semota, a Conical, Stout-Teethed Succulent

Comparison of Three Cytokinins on In vitro Multiplication of Orbea semota, a Conical, Stout-Teethed Succulent

An improved micropropagation protocol for the ex situ conservation of Thlaspi cariense A. Carlström (Brassicaceae): an endangered Turkish endemic plant species

Comparison of Three Cytokinins on In vitro Multiplication of Orbea semota, a Conical, Stout-Teethed Succulent

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