In vitro propagation of Epipactis flava Seidenf., an endangered rheophytic orchid: a first study on factors affecting asymbiotic seed germination, seedling development and greenhouse acclimatization

Kunakhonnuruk, Boworn; Inthima, Phithak; Kongbangkerd, Anupan

doi:10.1007/s11240-018-1475-9

Cited by 31 publications

(19 citation statements)

References 59 publications

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“…In vitro germination of orchids makes it possible to increase the efficiency of conservation and breeding programs, since in vitro germination rates higher than 70% are commonly reported [23], while in ex vitro conditions under natural environmental conditions, these rates hardly exceed 5% germinated seeds [24]. This is especially due to the fact that orchid seeds do not contain nutritional reserves [25], and the embryo and seedlings at early germination are highly dependent on symbiosis with microorganisms known as mycorrhizae, which nutritionally supply these plants during a long time until the complete establishment of the seedling in the natural environment [26].…”

Section: Introductionmentioning

confidence: 99%

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

Cardoso

Zanello

Chen

2020

IJMS

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The process through induction, proliferation and regeneration of protocorm-like bodies (PLBs) is one of the most advantageous methods for mass propagation of orchids which applied to the world floricultural market. In addition, this method has been used as a tool to identify genes of interest associated with the production of PLBs, and also in breeding techniques that use biotechnology to produce new cultivars, such as to obtain transgenic plants. Most of the molecular studies developed have used model plants as species of Phalaenopsis, and interestingly, despite similarities to somatic embryogenesis, some molecular differences do not yet allow to characterize that PLB induction is in fact a type of somatic embryogenesis. Despite the importance of species for conservation and collection purposes, the flower market is supported by hybrid cultivars, usually polyploid, which makes more detailed molecular evaluations difficult. Studies on the effect of plant growth regulators on induction, proliferation, and regeneration of PLBs are the most numerous. However, studies of other factors and new technologies affecting PLB production such as the use of temporary immersion bioreactors and the use of lighting-emitting diodes have emerged as new tools for advancing the technique with increasing PLB production efficiency. In addition, recent studies on Phalaenopsis equestris genome sequencing have enabled more detailed molecular studies and the molecular characterization of plantlets obtained from this technique currently allow the technique to be evaluated in a more comprehensive way regarding its real applications and main limitations aiming at mass propagation, such as somaclonal variation.

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Section: Introductionmentioning

confidence: 99%

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

Cardoso

Zanello

Chen

2020

IJMS

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“…The SSS medium possibly provided less nutrient absorption than TIS and CIS [16], while CIS frequently induced abnormality and necrosis symptoms resulting from permanent immersion [24]. Better growth and proliferation of E. flava plants cultured by CIS over SSS were reported by Kunakhonnuruk et al [3]; however, no differences were observed under shorter culturing time. Therefore, large-scale mass production of E. flava by CIS was not recommended, although this plant generally displays a rheophytic habit.…”

Section: Discussionmentioning

confidence: 90%

“…The germination of E. flava seeds obtained from green pod was performed under aseptic condition in 120 mL culture vessel containing 20 mL of semi-solid medium. After few months, in vitro seedlings of E. flava with two to three leaves were cultured on modified Murashige and Skoog medium [15] supplemented with 150 mL L −1 coconut water (CW), 50 g L −1 potato extract (PE) and 20 g L −1 sucrose [3] for two months before being used as explants. Explants of E. flava with two to three buds differentiated from rhizomes that were cultured in semi-solid system (SSS), continuous immersion system (CIS) and TIS using the same medium.…”

Section: Evaluation Of Different In Vitro Culture Systemsmentioning

confidence: 99%

“…A rheophytic lifestyle appears to be very rare in Orchidaceae, and the only rheophytic orchid found in Thailand is Epipactis flava [1]. Intricate morphological details and life cycle of E. flava were described by Pedersen et al [2] and Kunakhonnuruk et al [3], respectively. In Thailand, E. flava grows in streams with calcareous substrates [2].…”

Section: Introductionmentioning

confidence: 99%

“…Plant tissue culture is recognized as a high-performance tool for ex situ conservation of endemic and endangered orchid species [6][7][8]. Recently, successful in vitro seed germination and seedling culture of E. flava was reported [3]. However, for in vitro mass propagation, conventional techniques using semi-solid or shake-flask cultures are labor intensive during the subculturing period.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Propagation of Rheophytic Orchid, Epipactis flava Seidenf.—A Comparison of Semi-Solid, Continuous Immersion and Temporary Immersion Systems

Kunakhonnuruk

Inthima

Kongbangkerd

2019

Biology

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Epipactis flava Seidenf. is an endangered Thai rheophytic orchid that has recently shown a rapid decrease in its natural habitat, prompting an urgent need for conservation using ex situ reintroduction methods. Temporary immersion system (TIS) has been successfully applied for large-scale propagation in various plants species. Propagation efficiency of E. flava using TIS was investigated and compared with conventional semi-solid system (SSS) and liquid continuous immersion system (CIS). The highest percentage of new shoot and shoot bud formation was obtained from TIS, followed by CIS and SSS, respectively. Growth parameters as indicated by number of new shoots, shoot buds, shoot height and leaves per explant were significantly higher using TIS than with SSS and CIS. Moreover, the maximum number of new shoots and shoot buds per replication were reliably obtained from TIS higher than SSS and CIS. After acclimatization, the highest survival percentage of plantlets was observed in TIS (76.7%), with 60% surviving after eight weeks of transplantation in artificial stream. TIS was determined as the most suitable culture system for in vitro mass propagation of E. flava compared to CIS and SSS.

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Asymbiotic Seed Germination in Terrestrial Orchids: Problems, Progress, and Prospects

Anghelescu,

Vafaee,

Ahmadzadeh

et al. 2023

Advances in Orchid Biology, Biotechnology and Omics

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In vitro propagation of Epipactis flava Seidenf., an endangered rheophytic orchid: a first study on factors affecting asymbiotic seed germination, seedling development and greenhouse acclimatization

Cited by 31 publications

References 59 publications

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

In Vitro Propagation of Rheophytic Orchid, Epipactis flava Seidenf.—A Comparison of Semi-Solid, Continuous Immersion and Temporary Immersion Systems

Asymbiotic Seed Germination in Terrestrial Orchids: Problems, Progress, and Prospects

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