In vitro propagation of Ethiopian mustard (Brassica carinata A. BRAUN)

Critical Review on Plant Micropropagation of Ethiopian Plants Reported So Far: Existing Gaps, Required Standardization, and Future Research Direction

“…e stated objectives and results of the various micropropagation studies have wide coverage and include virus cleaning and disease-free plantlet production, screening for salt-tolerant plants, in vitro micropropagation of medicinal and high-value crops, identification of better microtuber induction under various levels of sucrose, optimization of various concentrations of sterilants and plant growth regulators, and in vitro conservations [10][11][12][13][14][15][16][17][18][19][20], [21][22][23][24][25][26][27][28][29][30][31], [32][33][34][35][36][37][38][39][40][41][42], [43][44][45][46][47][48][49][50][51][52][53][54][55]. Other objectives include hormonal and media treatments to overcome hyperhydricity (vitrification) of micropropagated shoots…”

“…ese studies identified MS media with some growth regulators individually and/or in combinations for the shoot and root developments [27,[37][38][39][40]42] and determined the minimum number of days for root induction [28], the highest percentage of callus production [29], effect of PGRs on the number of roots [45], and shoot length and shoot fresh weight [27,37,53,55]. e studies also determined the survival rate of micropropagated plants in greenhouses [38,45] and identified responsive explants and polyploidization, for example, to produce larger bulb size in garlic [26], by anticipating an increase in 4.…”

“…Plant micropropagation reports in Ethiopia should thus pay attention to these presumed controversies and contribute their share to address some of the existing controversies in order to expand the application of micropropagation with the desired repeatability and standards. Diverse plant species including cereals, oil crops, spices, medicinal plants, high-value crops, fruit plants, root crops, and endangered multipurpose shrubs were utilized in various forms of plant micropropagation in Ethiopia [10][11][12][13][14][15][16][17][18][19][20], [21][22][23][24][25][26][27][28][29][30][31], [32][33][34][35][36][37][38][39][40][41][42], [43][44][45][46][47][48][49][50][51][52][53][54][55]. Critical assessment of these studies would facilitate the development of repeata...…”