In vitro propagation of Eucalyptus grandis L. by tissue culture

Sita, G. Lakshmi; Rani, B. Shobha

doi:10.1007/bf00269207

Cited by 29 publications

(10 citation statements)

References 9 publications

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“…The production of synthetic auxins in the 20th century also led to research into their effects on adventitious root induction [152,153]. Much of this research has focused on determining the appropriate combinations of auxin concentration, application formulation, and application method that result in the highest percentages of shoots forming roots [109,154,155].…”

Section: Auxin Dynamics During Vegetative Propagationmentioning

confidence: 99%

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

Stuepp

Wendling

Trueman

et al. 2017

Forests

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Qualitative and quantitative hormone analyses have been essential for understanding the metabolic, physiological, and morphological processes that are influenced by plant hormones. Auxins are key hormones in the control of many aspects of plant growth and development and their endogenous levels are considered critical in the process of adventitious root induction. Exogenous auxins are used extensively in the clonal propagation of tree species by cuttings or tissue culture. Understanding of auxin effects has advanced with the development of increasingly accurate methods for auxin quantification. However, auxin analysis has been challenging because auxins typically occur at low concentrations, while compounds that interfere with their detection often occur at high concentrations, in plant tissues. Interference from other compounds has been addressed by extensive purification of plant extracts prior to auxin analysis, although this means that quantification methods have been limited by their expense. This review explores the extraction, purification, and quantification of auxins and the application of these techniques in developing improved methods for the clonal propagation of forestry trees.

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Section: Auxin Dynamics During Vegetative Propagationmentioning

confidence: 99%

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

Stuepp

Wendling

Trueman

et al. 2017

Forests

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“…Badia Nkanka (1982) noticed that the in vitro subcultures can have a strong effect on rooting ability of Eucalyptus when De Fossard's substrate was used. Recent studies, however, refer to the possibilities of using aged nodal segments as explants for in vitro multiplication of elite genotypes with good results (Lakshmi Sita and Rani 1985;Sankara Rao and Venkateswara 1985;Das and Mitra 1990), while the problem ofmicrobial contamination in ex vivo explants can be reduced by suitable in-field fungicidal treatments (Le Roux and Van Staden 1991). In his work, Badia Nkanka (1982) observed an improvement in E. rudis rooting ability in the presence ofvitamin E that acted as an antioxidizing agent against polyphenols coming from the tissues, and had at the same time a synergic effect with other medium growth regulators.…”

Section: Micropropagationmentioning

confidence: 99%

Medicinal and Aromatic Plants VIII

Bajaj¹

1995

Biotechnology in Agriculture and Forestry

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PrefaceThis series ofbooks on the biotechnology of Medicinal and Aromatic Plants provides a survey of the literature focusing on recent information and the state of the art in tissue culture and the in vitro production of secondary metabolites. This book, Medicinal and Aromatic Plants VIII, like the previous seven volumes published in 1988, 1989, 1991, 1993, and 1994, is unique in its approach. It comprises 26 chapters dealing with the distribution, importance, conventional propagation, micropropagation, tissue culture studies and the in vitro production of important medicinal and pharmaceutical compounds in various species of Achillea,

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“…Many reports in the existing literature on ill vitro production protocols of E. grandis (Lakshmi Sita and Shobha Rani, 1985), E. globulus and E. citfiodora (Mascarenhas et aI., 1982) did not clearly establish good survival rates in large-scale operations under field conditions. However, it was felt that in-house…”

Section: Micropropagation Of Eucalyptusmentioning

confidence: 99%

Applications of Biotechnology in Forestry and Horticulture

Dhawan¹

1990

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Land is the most fundamental resource of a country and as a result of the high rate of population growth in India, the pressure to produce more food, fodder, timber and fuelwood from the available land is intense. Land use, therefore, needs judicious planning. India has about 130 Mha of wastelands. Wastelands are defined as lands that are degraded and are lying unutilised due to constraints such as salinity, alkalinity, waterlogging, wind and water erosion, etc. Since wastelands are degraded and unsuitable for agriculture, they can be utilised to meet the growing demand for fuelwood, fodder, small timber and industrial wood. Not only do we have to reclaim the wastelands but we should also try and protect our forest cover which is being depleted at the rate of 1.3 Mha per year. Efforts are also required to step up the existing per hectare yield of 0.2 m 3 to 2-3 m 3 • The need of the hour is a quantum jump in existing afforestation targets. The plan is to afforest wastelands at a rate of 5 Mha per annum. Such large-scale plantations will demand a large amount of planting material (seeds or seedlings) and in order to achieve higher yields, it is important that 4 Ram achan dran these planting materials are of good quality. This means a high yielding plant which is also resistant to different diseases and pests. Such plants can be obtained by conventional methods of tree breeding and non-conventional methods of genetic engineering and somatic hybridisation. Unlike annual crops, the breeding behavior of trees is complex and their life-cycle long. Therefore, it takes many years to produce a variety with desirable traits. However, the development of modern non-conventional methods of genetic engineering, tissue culture and somatic hybridisation has offered new ways to enhance forest productivity more rapidly. By these methods, it has become possible to develop such varieties of ornamental and crop plants, with many desirable traits such as fast growth, high yield, disease resistance, herbicide and stress tolerance, etc. in a much shorter time than that taken by the conventional methods. However, this quick, non-conventional methodology of producing plants is very expensive. Since both the methods have their own advantages and disadvantages, it will be prudent to combine the conventional methods and the new methods of biotechnology to increase productivity, both qualitatively and quantitatively, in order to achieve the desired goals. CHOICE OF TREESForests constitute the base for agriculture, animal husbandry, industrial development and, thus, all-round development. A dense forest cover is also required for a healthy environment, as deforestation leads to environmental degradation, soil erosion, recurrent floods, droughts and desertification. India's forest area is about 64.20 Mha which constitutes about 19.52 percent of the total geographic area of the country (National Natural Resource Monitoring System, 1987, of the Department of Space, Government of India). With less than 2 percent of the world's forest area, we are ...

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In vitro propagation of Eucalyptus grandis L. by tissue culture

Cited by 29 publications

References 9 publications

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

The Use of Auxin Quantification for Understanding Clonal Tree Propagation

Medicinal and Aromatic Plants VIII

Applications of Biotechnology in Forestry and Horticulture

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