In Vitro Propagation of Fifty Olive Cultivars

Zuccherelli, G.; Zuccherelli, S.

doi:10.17660/actahortic.2002.586.204

Cited by 38 publications

(40 citation statements)

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“…Although the propagation of several fruit species difficult to multiply by traditional methods has been made possible by micropropagation, regarding the olive micropropagation, there is not yet enough knowledge for its use in a mass scale nursery production (Zuccherelli and Zuccherelli, 2002).…”

Section: Introductionmentioning

confidence: 99%

Coconut water and BAP successfully replaced zeatin in olive (Olea europaea L.) micropropagation

Peixe

Raposo

Lourenço

et al. 2007

Scientia Horticulturae

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The data presented report on trials conducted during 24 months using the Portuguese olive cultivar 'Galega vulgar'. The effectiveness of coconut water, BAP, or kinetin, as possible zeatin substitutes in olive micropropagation protocols, was investigated. In all stages of the micropropagation process, the mineral and vitamin formulation of olive medium (OM) was used. Regarding culture establishment the best results were achieved when 50 ml l À1 coconut water and 2.22 mM BAP were used as medium supplements. For the in vitro multiplication stage, the highest proliferation rates with an average of 3.4 new explants on each 30 days were achieved maintaining the coconut water concentration at 50 ml l À1 and increasing BAP up to 8.87 mM. The effects of IBA and activated charcoal on the in vitro root induction were also studied. Rooting rates of over 85% were obtained by basal immersion of the explants in IBA solution at 3 g l À1 for 10 s, followed by inoculation in the OM culture medium, added with 2 g l À1 of activated charcoal and without growth regulators. All in vitro rooted plants were transferred into Jiffy-Pots filled with vermiculite-perlite 3:1 (v/v) substrate. Those were subsequently wetted with the OM mineral solution, placed into polystyrene plates each one with 100 Jiffy-Pots capacity, which were transferred to traditional rooting mist benches, on a water-cooling equipped greenhouse. Such a simple acclimatization procedure allowed for 95% of plants survival. #

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Section: Introductionmentioning

confidence: 99%

Coconut water and BAP successfully replaced zeatin in olive (Olea europaea L.) micropropagation

Peixe

Raposo

Lourenço

et al. 2007

Scientia Horticulturae

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“…In the last decades, the intense research activity developed in micropropagation of Olea genus was almost restricted to commercial cultivars (e.g., Briccoli Bati et al 2006;Peixe et al 2007;Rugini 1984;Zacchini and De Agazio 2004;Zuccherelli and Zuccherelli 2002). However, there is not enough knowledge for its use in a mass-scale nursery production (Peixe et al 2007;Zuccherelli and Zuccherelli 2002).…”

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confidence: 99%

Large-scale field acclimatization of Olea maderensis micropropagated plants: morphological and physiological survey

Brito

Costa

Coelho

et al. 2009

Trees

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Micropropagation allows large-scale plant multiplication and germplasm preservation, representing an added value in forest breeding strategies to combat desertification and/or protect endangered species. We developed a large-scale micropropagation protocol of Olea maderensis (a native endangered wild olive of Madeira Archipelago) using OMG medium (rich in Fe, Mg and Mn) supplemented with zeatin for elongation and with NAA for rooting. We now describe the performance of micropropagated plants during five-period field acclimatization: (1) in vitro, (2) growth-cabinet, (3) greenhouse, (4) open-greenhouse, and (5) field mountain in Porto Santo Island. One hundred OG4 plants were acclimatized, showing [95% surviving rates. During acclimatization, several physiological parameters were evaluated; water content remained higher in in vitro/greenhouse conditions, decreased in field leaves. Soluble protein contents decreased during the first acclimatization periods increasing thereafter. Membrane permeability slightly increased during the field acclimatization. Chlorophylls content increased in in vitro leaves, while during acclimatization, mostly chl b decreased, increasing chl a/chl b ratio. F 0 decreased in first acclimatization periods, increasing thereafter, while the other parameters (F v ; F m ; F v /F m ) decreased. Nutrient contents decreased in plants transferred to poor field soil conditions, reaching values similar to mother plant leaves. Overall, with the exception of PSII fluorescence, field acclimatized plants had similar values to mother plants, showing a good adjustment to stressful field conditions. This protocol is being used in large-scale micropropagation within a reforestation program, and is an example of R&D technologies with immediate application on protection of endangered ecosystems.

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“…The medium was formulated by analyzing the mineral element contained in the mature seeds. This medium is currently used for propagation of more than 50 olive cultivars in commercial plant production in Italy (Zuccherelli and Zuccherelli, 2000). The axillary bud stimulation allows the rapid growth, better performance, and higher qualitative characteristics of the in vitro-grown plants than plants derived from propagation by stem cutting.…”

Section: Axillary Bud Stimulationmentioning

confidence: 99%

Olive

Rugini

Gutiérrez-Pesce

Muleo

2008

Compendium of Transgenic Crop Plants

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Biochemical and molecular procedures have been developed for olive genotyping, for taxonomy between cultivated and wild olives, for phylogenetic studies of cultivars, and for identification of usable markers linked to the most important agronomic traits, such as size of the tree, flowering induction, apical dominance, productivity, self‐fertility, quantity and quality of oil and secondary products of fruit, biotic and abiotic resistance. These characters could be introgressed into cultivars by conventional breeding or through transgeny. Successful results by classical breeding are difficult to obtain due to the different flower fertility and the long juvenile period of the progenies. The low efficiency of modern genetic improvement techniques suggests that genetic transformation techniques could speed up the development of new genotypes; however, the development of efficient regeneration methods from mature tissue is the limiting factor for applying this technology, in olive. Transgenic olive plants with modified growth habit and putative induced disease resistance are under filed conditions to test their phenotype. Other attempts of genetic improvement such as clonal selection, mutation through γ‐irradiation, protoplast technology, haploid, changes in ploidy level, somaclonal variation, and somatic hybridization are discussed and reviewed.

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In Vitro Propagation of Fifty Olive Cultivars

Cited by 38 publications

References 0 publications

Coconut water and BAP successfully replaced zeatin in olive (Olea europaea L.) micropropagation

Coconut water and BAP successfully replaced zeatin in olive (Olea europaea L.) micropropagation

Large-scale field acclimatization of Olea maderensis micropropagated plants: morphological and physiological survey

Olive

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