In vitro Propagation of Four Grape Cultivars

Abstract

Successful propagation of in vitro four grape cvs. (Concord, Thompson Seedless, Beauty Seedless and King Ruby) through shoot tips and nodal cuttings was established. The best explants sterilization was achieved with 10% cholorox for 10 minutes. The combination of 1.0 mg/L BA and 0.01 mg/L NAA produced the maximum sprouting rate (100, 91.7, 100, 83.3%) and the least number of days for buds sprouting (6, 9, 5 and 11) of Concord, Thompson Seedless, Beauty Seedless and king Ruby cultivars, respectively. Additionally, the same medium induced the greatest average of shoots number/explants (5.50, 4.95, 5.27 and 4.69) and the highest shoot length (5.3, 5.2, 5.2 and 4.9 cm) for the four tested grape cvs. The third subculture induced the maximum response of sprouting frequency and the optimum average of shoot numbers/explants for the four examined cultivars. For rooting of in vitro shoots, Half strength MS medium supplied with 1.0 mg/L IBA resulted in the best root formation (90, 100, 80 and 70%) for Concord, Thompson Seedless, Beauty Seedless and King ruby cultivars, respectively.