In vitro propagation of herbaceous peony (paeonia lactiflora pall.) by a longitudinal shoot-split method

Abstract

A procedure for the clonal propagation ofPaeonia lactiflora Pall. cvs. Takinoyosooi and Sarah Bernhardt through shoot tip culture is described. Half strength Murashige and Shoog (1962) medium supplemented with 0.5 mg/l 6-benzylaminopurine plus 1 mg/l gibberellic acid promoted formation and growth of axillary buds. Continuous shoot multiplication was achieved by vertically splitting the shoot axis and subsequent division of elongated axillary shoots every 36 days. High frequency (57-100%) of rooting was obtained on paper-bridge liquid medium supplemented with 1 mg/l indole-3-butyric acid. Half of the rooted plantlets were established on porous soil. Thus, 700 and 300 plants of cv. Takinoyosooi and Sarah Bernhardt could be theoretically obtained from a single bud in one year.

Direct shoot induction ofPaeonia lactiflora‘Zhong Sheng Fen’ and rejuvenation of hyperhydric shoots

“…(Hosoki et al 1989;Gabryszewska 1998;Guo 2001;Ding et al 2010). BA is the most commonly used cytokinin for peony shoot proliferation (Hosoki et al 1989;Bouza et al 1994;Gabryszewska 1998).…”

“…(Hosoki et al 1989;Gabryszewska 1998;Guo 2001;Ding et al 2010). BA is the most commonly used cytokinin for peony shoot proliferation (Hosoki et al 1989;Bouza et al 1994;Gabryszewska 1998). In the study of Paeonia suffruticosa, 6-benzyladenine (BAP) was the only cytokinin effective for leaf and axillary bud development and, when combined with another cytokinin, reduced its efficiency (Bouza et al 1994).…”

“…However, the multiplication rate by this method is too low to meet the needs of modern mass production. Thus, direct or indirect shoot induction have been the major objectives of several studies (Hosoki et al 1989;Orlikowska et al 1998;Guo 2001;Tian et al 2010;Wang et al 2010). Tissue culture and rapid propagation of herbaceous peony can augment the propagation rate and shorten the breeding period, which is important for production.…”

Multiple shoot induction and rooting of Paeonia lactiflora ‘Da Fu Gui’

“…According to a recent study, only hypocotylderived callus successfully produced adventitious shoots, but at a low regeneration rate (7.95%) . Direct shoot induction is more prevalent in peony in vitro culture with several successful cases reported for both herbaceous peony (Hosoki et al, 1989;Gabryszewska, 1998) and tree peony (Bouza et al, 1994a, b;Kong and Zhang, 1998). Buds, shoot tips, nodal stem, petioles and leaf segments can be used for direct shoot induction; the highest frequency of shoot induction has been found in vegetative axillary buds or shoot tips (Hosoki et al, 1989;Orlikowska et al, 1998;Guo, 2001;Hu et al, 2003;Tian et al, 2010).…”

“…Direct shoot induction is more prevalent in peony in vitro culture with several successful cases reported for both herbaceous peony (Hosoki et al, 1989;Gabryszewska, 1998) and tree peony (Bouza et al, 1994a, b;Kong and Zhang, 1998). Buds, shoot tips, nodal stem, petioles and leaf segments can be used for direct shoot induction; the highest frequency of shoot induction has been found in vegetative axillary buds or shoot tips (Hosoki et al, 1989;Orlikowska et al, 1998;Guo, 2001;Hu et al, 2003;Tian et al, 2010). Many methods were reported to enhance axillary shoot formation, such as the addition of thidiazuron (TDZ) at very low concentrations (0.01 to 0.04 mg l -1 ) to the medium containing a mixture of 6-benzyladenine (BA) (1, 2 and 4 mg l -1 ) + 6-γ-γ-dimethylaminopurine (2iP) (1, 2 and 4 mg l -1 ) + kinetin (Kin) (1, 2 and 4 mg l -1 ), a longi-tudinal shootsplit method in subculture (Hosoki et al, 1989;Gabryszewska, 1998) and manipulation of the light spectral quality under photoautotrophic conditions (Ding et al, 2010).…”