In vitro propagation of<i>Calamintha nepeta</i>

Vlachou, G.; Papafotiou, Μ.; Bertsouklis, Κ.F.

doi:10.17660/actahortic.2016.1113.28

Cited by 7 publications

(11 citation statements)

References 12 publications

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“…In vitro rooting and ex vitro acclimatization and establishment. It has been reported that C. nepeta microshoots rooted at higher percentage on Hf-halfstrength MS medium compared with the full-strength medium (Vlachou et al, 2016b). In the present study, adult-and seedling-origin microshoots produced on various multiplication media, rooted promptly (92% to 100%) on half-strength MS medium either Hf (Fig.…”

Section: Resultssupporting

confidence: 61%

“…Effect of cytokinin type and concentration on in vitro shoot multiplication. C. nepeta cultures from adult wild plants were established on MS medium supplemented with 1.0 mg • L -1 BA (70% contamination, 75% explant response for shoot production, 2-3 shoots per responding explant, data presented by Vlachou et al, 2016b), whereas Pistelli et al (2013) used MS with 0.5 mg • L -1 BA for initial establishment of C. nepeta. A considerable number of Mediterranean xerophytes, including species of the Lamiaceae family, cultured on a BA-supplemented MS medium responded better at lower BA concentrations (i.e., 0.25 to 0.5 mg • L -1 ) compared with higher BA concentrations (1.0 to 4.0 mg • L -1 ) (Papafotiou et al, 2017b).…”

Section: Resultsmentioning

confidence: 99%

“…The in vitro cultures were initiated from shoot tip explants excised from adult wild plants (see previously) and cultured on MS medium supplemented with 1.0 mg • L -1 BA. Details on this are given in Vlachou et al (2016b). The cultures were maintained with a number of subcultures on the initiation medium followed by one subculture on Hf-MS medium.…”

Section: Micropropagationmentioning

confidence: 99%

“…Based on results of a previous study of ours (Vlachou et al, 2016b), microshoots, 2.0 to 2.5 cm long, were transferred on halfstrength MS medium either Hf or supplemented with 0.5, 1.0, 2.0, or 4.0 mg • L -1 IBA. Also, microshoot clusters produced on multiplication media were put on Hf-halfstrength MS medium for rooting.…”

Section: In Vitro Rootingmentioning

confidence: 99%

“…There is limited published work on clonal propagation of C. nepeta. In a preliminary publication, we reported in vitro culture of the species, starting from adult plants, on MS medium supplemented with ZEA or BA, with rather low shoot proliferation (Vlachou et al, 2016b).…”

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confidence: 99%

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Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

Vlachou¹,

Papafotiou²,

Bertsouklis³

2019

horts

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Seed ecophysiology and micropropagation of Clinopodium nepeta, an aromatic Mediterranean plant with pharmaceutical and horticultural uses was investigated. The optimum germination temperature of seeds stored at room temperature for 0, 6, or 12 months was 15 to 20 °C (100% germination completed in 10 to14 days) and cardinal temperatures were defined at 10 and 30 °C (80% to 82% and 62% to 76% germination, respectively). Six or 12 months of storage did not seem to affect germination, although 12-month-old seeds germinated at higher percentage and completed germination earlier at 15 °C than at 20 °C. Concerning micropropagation, shoot multiplication at subcultures of both adult plant- and seedling-origin nodal explants was tested on Murashige and Skoog (MS) medium supplemented with various cytokinin types, i.e., zeatin (ZEA), 6-benzyladenine (BA), kinetin (KIN), and 6-γ-γ-(dimethylallylamino)-purine (2IP), at various concentrations from 0.0 to 8.0 mg·L−1. Both explant types presented a rather similar response during in vitro culture. Increasing concentration of all cytokinin types resulted in an increase in shoot number per responding explant (1.1–5.3) and in most cases a decrease in shoot length (0.6–3.4 cm). Increasing cytokinin concentration induced hyperhydricity to a number of shoots (0.1–6.5) per explant, mostly when ZEA and BA were used. Supplementing the MS medium with 8.0 mg·L−1 BA combined with 0.1 mg·L−1 1-naphthaleneacetic acid (NAA) led to almost elimination of hyperhydricity and very satisfactory shoot production (80%/88% explant response and 6.5/7.5 shoot number per responding explant for seedling- / adult-origin explants, respectively). Alternatively, increasing the agar concentration to 12.0 g·L−1 and supplementing the medium with 8.0 mg·L−1 BA only, resulted in the same effect on eliminating hyperhydricity, such as the addition of NAA, and in the best shoot multiplication response achieved in this study (100% explant response, 9.4/9.9 shoots per explant for seedling-/adult-origin explants, respectively). Microshoots rooted abundantly (92% to 100%) on half-strength MS medium, either Hf or supplemented with 0.5 mg·L−1 to 4.0 mg·L−1 indole-3-butyric acid (IBA). The addition of IBA to the rooting medium, regardless of its concentration, affected only the root length by increasing it 2- to 3-fold. Microshoot clusters produced on multiplication media rooted at 96% when cultured on Hf half-strength MS medium. Rooted microshoots and shoot clusters survived at 80% to 100%, respectively, after ex vitro acclimatization in peat:perlite 1:1 (v/v).

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Section: Resultssupporting

confidence: 61%

Section: Resultsmentioning

confidence: 99%

Section: Micropropagationmentioning

confidence: 99%

Section: In Vitro Rootingmentioning

confidence: 99%

mentioning

confidence: 99%

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Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

Vlachou¹,

Papafotiou²,

Bertsouklis³

2019

horts

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Seed germination, micropropagation from adult and juvenile origin explants and address of hyperhydricity of the Cretan endemic herb Calamintha cretica

Vlachou

Papafotiou

Bertsouklis

2020

Not Bot Horti Agrobo

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The optimum range of temperature for germination (96-100%) of Calamintha cretica, an herb with potential pharmaceutical and horticultural uses, was 15 to 20 °C, with 10 and 30 °C cardinal temperatures. Storage up to one year did not affect germination. The effect of zeatin (ZEA), 6-benzyladenine (BA), kinetin, and 6-γ-γ-(dimethylallylamino)-purine added in MS medium at concentrations from 0.0 to 8.0 mg L-1 was tested for shoot proliferation of both adult- and seedling-origin nodal explants at first- and sub-culture. Both explant types responded similarly during in vitro culture. At cytokinin concentrations up to 1 mg L-1 explant response was high (over 85%) but shoot number per explant was low (1.2-2.2). Increasing cytokinin from 2.0 to 8.0 mg L-1 resulted to an analogous decrease of explant response and shoot length, and an increase of shoot number, particularly when ZEA or BA was used (5.0-6.6 shoots per explant, 0.5-1.0 cm long) with simultaneous though increase of hyperhydricity (up to 50%). The addition of 0.1 mg L-1 naphthaleneacetic acid into the 8.0 mg L-1 BA medium almost eliminated hyperhydricity and increased explant response, while the increase of agar concentration from 8.0 to 12.0 g L-1 eliminated hyperhidricity and induced the highest shoot proliferation (93-95% explant response, 11.2-12.3 shoots per explant, 0.8-1.0 cm long). Microshoots and microshoot clusters rooted (88-96%) on half-strength MS medium either hormone free or supplemented with 1 to 4 mg L-1 indole-3-butyric acid. Plantlets survived at 80% to 100% after ex vitro acclimatization in peat: perlite 1:1 (v/v).

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Integrated design and management of vegetation at archaeological sites to protect monuments and enhance the historical landscape

Papafotiou¹,

Kanellou²,

Economou³

2017

Acta Hortic.

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In vitro propagation ofCalamintha nepeta

Cited by 7 publications

References 12 publications

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

Studies on Seed Germination and Micropropagation of Clinopodium nepeta: A Medicinal and Aromatic Plant

Seed germination, micropropagation from adult and juvenile origin explants and address of hyperhydricity of the Cretan endemic herb Calamintha cretica

Integrated design and management of vegetation at archaeological sites to protect monuments and enhance the historical landscape

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