In Vitro Propagation of Lily (Lilium longiflorum Thunb.) with Growth Regulators BAP and NAA

Deswiniyanti, Ni Wayan; Lestari, Ni Kadek Dwipayani; Astarini, Ida Ayu; Hardini, Yunita

doi:10.29244/jhi.11.2.131-139

Cited by 4 publications

(5 citation statements)

References 7 publications

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“…Sehingga kalus yang bersifat embrio somatik berpotensi meningkatkan rekayasa secara molekuler melalui rekayasa genetika (Tikendra et al 2019;Asmono et al 2020). Berdasarkan literatur tersebut, hasil penelitian ini diharapkan kalus embriogenik akan menghasilkan tunas yang stabil, dan persentase transformasi terhadap kalus tersebut dapat meningkat (Deswiniyanti et al 2020;Pramono et al 2021;Ebrahimzadegan dan Maroufi 2022). Sehingga hal tersebut dapat meningkatkan jumlah tunas transgenik yang stabil dalam pewarisan gen pada generasi selanjutnya.…”

Section: Hasil Dan Pembahasanunclassified

Kombinasi BAP dan NAA untuk Media Perbanyakan Nanas Varietas Smooth Cayenne, Toboali in Vitro

Dahniar

Elvavina

2022

Agrotechnology Res J

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Toboali pineapple variety is a smooth cayenne variety because it contains high antioxidants in the form of vitamins A and C. In addition, it is a source of citric and malic acids, substances that can be used to enhance the taste of the fruit. Pineapple is a plant that is propagated with vegetative organs such as suckers, slips, and crowns. However, conventional propagation has a low propagation rate. Tissue culture is an in vitro technique used in the rapid propagation of plants and in large quantities to meet the needs of cultivation and research. The selection of regeneration media is very influential on the development of plants in tissue culture. This study aimed to obtain the optimal media combination with BAP and NAA in the regeneration of Smooth Cayenne pineapple (Toboali) in vitro for mass propagation of seedlings and genetic engineering research. This study used 6 levels of BAP, namely 0, 1, 2, 3, 4, 5 mg.L-1 and 4 levels of NAA, namely 0, 1, 2, 3 mg.L-1. The treatment used was a combination of BAP and NAA as many as 24 treatments with 3 replications. The best pineapple regeneration medium for mass plant propagation in this study was BAP 3 mg.L-1 without NAA media which produced the highest shoots of 17.33 shoots per explant at 12 week after planting. The best pineapple regeneration medium for genetic engineering research was a combination BAP 3 mg.L-1 and NAA 2 mg.L-1 media which produced 18.33 shoots per explant from embryogenic callus.

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Section: Hasil Dan Pembahasanunclassified

Kombinasi BAP dan NAA untuk Media Perbanyakan Nanas Varietas Smooth Cayenne, Toboali in Vitro

Dahniar

Elvavina

2022

Agrotechnology Res J

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“…Beberapa tantangan dalam perbanyakan L. longiflorum di antaranya adalah individu selfincompatible (Anggraeni & Iriawati 2017), hermaprodit dan heterostyly (Deswiniyanti et al 2012). Perbanyakan Lilium secara generatif melalui biji menjadi kurang optimal karena ukuran biji yang relatif kecil, memiliki tingkat perkecambahan yang lambat dan menghasilkan variabilitas genetik yang tidak diinginkan (Ali et al 2013), serta pengadaan biji tergantung musim (Bakhshaie et al 2016).…”

Section: Pendahuluanunclassified

Induksi Kalus Sisik Umbi Lilium longiflorum Thunb. oleh Auksin dan Sitokinin, serta Respons Pertumbuhannya Secara In Vitro

Ekawati¹,

Anggraeni

Prawestri

et al. 2022

agrosainstek

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Lilium longiforum Thunb. adalah florikultura potensial untuk dikembangkan di bidang industri farmasi dan florikultura. Perbanyakan L. longiflorum secara generatif sulit dilakukan dan perbanyakan vegetatif dengan kultur jaringan jauh lebih efektif. Oleh karena itu, diperlukan sebuah protokol perbanyakan L. longiflorum secara in vitro yang efisien. Tujuan penelitian ini adalah mengamati induksi kalus dari eksplan sisik umbi dari planlet L. longiflorum dan respons pertumbuhannya terhadap penambahan auksin dan sitokinin dalam media kultur. Respons sisik umbi pada induksi kalus diuji dengan dua perlakuan, yaitu MS + 3,0 mg L-1 2,4-D + 0,5 mg L-1 BAP dengan inkubasi dalam keadaan 24 jam gelap (perlakuan 1) dan MS + 1,5 mg L-1 2,4-D + 1,0 mg L-1 BAP dengan fotoperiode 16/8 jam (perlakuan 2), selama 28 minggu. Kemudian, respons regenerasi kalus menjadi tunas diuji dengan penanaman kalus pada media regenerasi (MS + 3,4 mg L-1 BAP + 0,09 mg L-1 NAA) selama 12 minggu. Hasil penelitian menunjukkan bahwa eksplan pada perlakuan 2 lebih responsif untuk menginduksi kalus dari sisik umbi L. longiflorum dibandingkan eksplan pada perlakuan 1. Kalus yang dihasilkan bertekstur kompak dan berwarna hijau kekuningan dengan tingkat kesintasan 100% dan daya proliferasi yang tinggi. Media regenerasi berhasil meregenerasikan kalus menjadi tunas sebesar 100%, meskipun tidak terdapat pertumbuhan akar dalam penelitian ini. Perlakuan MS + 1,5 mg L-1 2,4-D + 1,0 mg L-1 BAP dengan fotoperiode 16/8 jam direkomendasikan sebagai sebuah protokol yang efektif dalam pengembangan L. longiflorum.

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“…The growth of cuttings can be stimulated by providing nutrients through the soil or directly to the plants. Growth regulators are used to encourage the formation of roots on cuttings such as auxin (Deswiniyanti et al 2020). There are various auxin growth regulators, namely IAA, IBA, and NAA (Shekhawat et al 2021).…”

Section: Introductionmentioning

confidence: 99%

Response of Growth and Yield of Mint (Mentha spicata L.) Cuttings to Auxin and Composition of Planting Media

Triharyanto,

Pujiasmanto,

Pardono

et al. 2023

Agrotechnology Res J

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Consumption of herbal medicines by Indonesian people is increasing because of the price and ease of obtaining them. One of the main ingredients of herbal medicine is mint leaves because they contain tannin and flavonoid secondary metabolites. However, the generative mint plant is very slow and needs vegetative cuttings propagation. This study aims to examine the effect of auxin-type growth regulators and the composition of the growing media on the growth and yield of mint plant cuttings. This study used a complete randomized block design with two factors. The first factor was the auxin growth regulator with four levels: without growth regulator, IBA 500 ppm, IAA 500 ppm, NAA 500 ppm. The second factor was the composition of the planting medium with three levels, namely soil, husk charcoal, and goat manure 3:3:1; 3:2:2, and 3:1:3. Three repetitions were carried out. Auxin affects the number and area of leaves. Applying 500 ppm NAA encouraged the highest leaf growth, with the number of leaves at 163.38. Growth regulator NAA 500 ppm increased the number of leaves and leaf area of mint plants, i.e., by 0.01% and 1.16% compared to the control. The growing media composition affected the crown fresh weight, root biomass, and crown root ratio. The media composition of 3:3:1 produced a crown fresh weight of 41.07 g, and the media composition 3:3:3 produced the highest biomass of 5.97 g. The media composition 3:1:3 showed the highest root crown ratio, 2.69%. Auxin and the composition of the growing media synergistically promote the growth and yield of mint cuttings.

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In Vitro Propagation of Lily (Lilium longiflorum Thunb.) with Growth Regulators BAP and NAA

Cited by 4 publications

References 7 publications

Kombinasi BAP dan NAA untuk Media Perbanyakan Nanas Varietas Smooth Cayenne, Toboali in Vitro

Kombinasi BAP dan NAA untuk Media Perbanyakan Nanas Varietas Smooth Cayenne, Toboali in Vitro

Induksi Kalus Sisik Umbi Lilium longiflorum Thunb. oleh Auksin dan Sitokinin, serta Respons Pertumbuhannya Secara In Vitro

Response of Growth and Yield of Mint (Mentha spicata L.) Cuttings to Auxin and Composition of Planting Media

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